Citations with the tag: CHITINASE
Results 1 - 50
- Occurrence and Activity of the Chitinolytic Bacteria of Aeromonas Genus.
Brzezinska, M. Swiontek; Donderski, W. // Polish Journal of Environmental Studies; 2001, Vol. 10 Issue 1, p27
Reports on the findings of a study on the occurrence and activity of chitinolytic planktonic bacteria of Aeromonas genus isolated from lake Jeziorak in Poland. Number of strains decomposing colloidal chitin; Effect of pH influence on chitinases activity; Influence of incubation time on total...
- Purification and some properties of a chitinase isozyme from the liver of Japanese common squid Todarodes pacificus.
MATSUMIYA, MASAHIRO; MIYAUCHI, KOUJI; MOCHIZUKI, ATSUSHI // Fisheries Science; Apr2003, Vol. 69 Issue 2, p427
Describes the purification and properties of chitinase isozyme from the liver of Japanese common squid Todarodes pacificus. Purification steps; Hydrolization of chitin to N-acetylchitooligosaccharides; Chitinase activity; Hydrolysis of chitosan.
- Cell and Molecular Biology, Biochemistry and Molecular Physiology. Three major somatic embryogenesis related proteins in identified as PR proteins.
Helleboid, St�phane // Journal of Experimental Botany; Jul2000, Vol. 51 Issue 348
Reports on the direct somatic embryogenesis process in leaf tissues of the Cichorium hybrid clone '474' which is accompanied by an overall increase in the amount of proteins secreted into the culture medium. Protein bands found in the conditioned medium; Acidic chitinases present in the...
- Erratum to: Quantification of chitinase and thaumatin-like proteins in grape juices and wines.
Le Bourse, D.; Conreux, A.; Villaume, S.; Lameiras, P.; Nuzillard, J.-M.; Jeandet, P. // Analytical & Bioanalytical Chemistry; Dec2011, Vol. 401 Issue 5, p1717
A correction to the article "Quantification of Chitinase and Thaumatin-Like Proteins in Grape Juices and Wines" that was published in a 2011 issue is presented.
- A new biomarker in asthma?
Scott, Ian // Thorax; Apr2008, Vol. 63 Issue 4, p365
The article presents a study on the evaluation of the pathogenic role of chitinase-like protein YKL-40 in asthma. Serum YKL-40 levels were measured and investigated in a case-control study which involves three separate cohorts of patients with asthma from Yale, Paris and Wisconsin. It was found...
- Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01.
Xia, Jin-Lan; Xiong, Jing; Zhang, Rui-Yong; Liu, Ke-Ke; Huang, Bin; Nie, Zhen-Yuan // Indian Journal of Microbiology; Sep2011, Vol. 51 Issue 3, p301
Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and...
- Purification, Characterization, and cDNA Cloning of Rice Class III Chitinase.
Hee-Young Park; Cheol-Ho Pan; Min-Young So; Joong-Hoon Ahn; Do-Hyun Jo; Su-Il Kim // Molecules & Cells; 2002, Vol. 13 Issue 1, p69
Presents a study on the expression of chitinases in a rice cell suspension culture. Methodology; Results; Discussion.
- Erratum: Theor Appl Genet (2001) 103:297â€“306.
Hee-Young Park; Cheol-Ho Pan; Min-Young So; Joong-Hoon Ahn; Do-Hyun Jo; Su-Il Kim // Theoretical & Applied Genetics; Dec2001, Vol. 103 Issue 8, p1312
Presents a correction to the article "Induced Expression of the Class II Chitinase Gene During Cold Acclimation and Dehydration of Bermudagrass," that was previously published in the periodical "Theory of Applied Genetics."
- Antibiotics and enzymes produced by the biocontrol agent Streptomyces violaceusniger YCED-9.
Trejo-Estrada, S R; Paszczynski, A; Crawford, D L // Journal of Industrial Microbiology & Biotechnology; Aug98, Vol. 21 Issue 1/2, p81
Streptomyces violaceusniger strain YCED-9 is an antifungal biocontrol agent antagonistic to many different classes of plant pathogenic fungi. We discovered that strain YCED-9 produces three antimicrobial compounds with antifungal activity. These compounds were purified and identified, and...
- Characterization of 38 kDa and 42 kDa chitinase isozymes from the liver of Japanese common squid Todarodes pacificus.
MATSUMIYA, Masahiro; MIYAUCHI, Kouji; MOCHIZUKI, Atsushi // Fisheries Science; Jun2002, Vol. 68 Issue 3, p603
Characterization was investigated on the 38 kDa and 42 kDa chitinase (EC220.127.116.11) isozymes from the liver of Japanese common squid Todarodes pacificus . Optimum pH toward colloidal chitin was observed at pH 3.0 for the 38 kDa chitinase, and pH 3.0 and 9.0 for the 42 kDa chitinase. K m and k cat...
- PRODUCTION OF CHITINASE BY STREPTOMYCES HYGROSCOPICUS VMCH2 BY OPTIMISATION OF CULTURAL CONDITIONS.
PRIYA, C. S.; JAGANNATHAN, N.; KALAICHELVAN, P. T. // International Journal of Pharma & Bio Sciences; Apr-Jun2011, Vol. 2 Issue 2, pB.210
A total of 10 chitinolytic bacteria were isolated from 35 soil samples collected from different crop fields in Tamilnadu state, India. Among them, a strain designated as VMCH2 which produced highest chitinolytic activity in primary and secondary screening in colloidal chitin agar was selected...
- SCREENING OF MARINE VIBRIO SP ISOLATED FROM THE BAYOF BENGAL, INDIA FOR CHITINASE ENZYME PRODUCTION.
RAVIKUMAR VINAYAGAM; MEIGNANALAKSHMI SUNDARAM // International Journal of Pharma & Bio Sciences; Apr-Jun2013, Vol. 4 Issue 2, pB-238
A total of 12 water, sediment and plankton samples were collected from different sampling stations of open sea off the Bay of Bengal coast of Chennai, Tamilnadu, India and 160 isolates of marine Vibrio sp were isolated using Thiosulphate Citrate Bile Sucrose (TCBS) agar medium. The entire...
- Detection and characterization of chitinases and other chitin-modifying enzymes.
Howard, Michael B.; Ekborg, Nathan A.; Weiner, Ronald M.; Hutcheson, Steven W. // Journal of Industrial Microbiology & Biotechnology; Nov2003, Vol. 30 Issue 11, p627
Multiple industrial and medical uses of chitin and its derivatives have been developed in recent years. The demand for enzymes with new or desirable properties continues to grow as additional uses of chitin, chitooligosaccharides, and chitosan become apparent. Microorganisms, the primary...
- A chitinase with high activity toward partially N-acetylated chitosan from a new, moderately thermophilic, chitin-degrading bacterium, Ralstonia sp. A-471.
Sutrisno, A.; Ueda, M.; Abe, Y.; Nakazawa, M.; Miyatake, K. // Applied Microbiology & Biotechnology; Jan2004, Vol. 63 Issue 4, p398
A moderately thermophilic bacterium, strain A-471, capable of degrading chitin was isolated from a composting system of chitin-containing waste. Analysis of the 16S rDNA sequence revealed that the bacterium belongs to the genus Ralstonia. A thermostable chitinase A (Ra-ChiA) was purified from...
- Biotechnological aspects of chitinolytic enzymes: a review.
Dahiya, Neetu; Tewari, Rupinder; Hoondal, Gurinder Singh // Applied Microbiology & Biotechnology; Aug2006, Vol. 71 Issue 6, p773
Chitin and chitinases (EC 18.104.22.168) have an immense potential. Chitinolytic enzymes have wide-ranging applications such as preparation of pharmaceutically important chitooligosaccharides and N-acetyl d-glucosamine, preparation of single-cell protein, isolation of protoplasts from fungi and...
- Purification, Crystallization and Preliminary Crystallographic Studies of a Class Ii Chitinase from Kidney Bean Seeds.
Zhaocai Zhou; Hanjian Liu, Z.; Yikun Li; Weimin Gong // Protein & Peptide Letters; Jun2002, Vol. 9 Issue 3, p265
A new member of class II chitinase from Phaseolus vulgaris was purified and crystallized. Diffraction data to 2.7ï¿½ resolution have been collected and the preliminary crystallographic studies have been completed. The space group is P1 with unit cell parameters of a=36.32ï¿½,...
- Response of transgenic cucumber expressing a rice class I chitinase gene to two fungal pathogens with different infectivities.
Kishimoto, Kyutaro; Nakajima, Masami; Nishizawa, Yoko; Tabei, Yutaka; Hibi, Tadaaki; Akutsu, Katsumi // Journal of General Plant Pathology; Dec2003, Vol. 69 Issue 6, p358
A transgenic cucumber line (CR32) over-expressing the rice class I chitinase gene exhibited resistance to Phytophthora rot (Phytophthora nicotianae var. parasitica) but not to Fusarium wilt (Fusarium oxysporum f. sp. cucumerinum). The infection behavior of these fungi on CR32 and nontransgenic...
- Production and Purification of Chitinase by Streptomyces sp. from Soil.
Sowmya, Balakrishnan; Gomathi, Duraisamy; Kalaiselvi, Manokaran; Ravikumar, Ganesan; Arulraj, Chinthamani; Uma, Chandrasekar // Journal of Advanced Scientific Research; Aug2012, Vol. 3 Issue 3, p25
Microbial production of chitinase has captured the worldwide attention of both industrial and scientific environments, not only because of its wide spectrum of applications but also for the lacuna of an effective production method. Chitinase production by a terrestrial Streptomyces species was...
- Thermodynamics of Chitinase Partitioning in Soy Lecithin Liposomes and Their Storage Stability.
Cano-Salazar, Luc�a; Ju�rez-Ord�z, Antonio; Gregorio-J�uregui, Karla; Mart�nez-Hern�ndez, Jos�; Rodr�guez-Mart�nez, Jes�s; Ilyina, Anna // Applied Biochemistry & Biotechnology; Dec2011, Vol. 165 Issue 7/8, p1611
The goal of this study was to define the partitioning behavior of chitinase from Trichoderma spp. in soy lecithin liposomes, using a thermodynamic approach based on the partitioning variation with temperature. An effort has been made to define the liposomes, as well as free and immobilized...
- Acidic mammalian chitinase is not a critical target for allergic airway disease in mice.
Fitz, L. J.; DeClercq, C.; Brooks, J.; Kuang, W.; Bates, B.; Demers, D.; Winkler, A.; Nocka, K.; Jiao, A.; Greco, R. M.; Mason, L. E.; Fleming, M.; Quazi, A.; Wright, J.; Goldman, S.; Hubeau, C.; Williams, C. M. // Journal of Inflammation;
An abstract of the article "Acidic mammalian chitinase is not a critical target for allergic airway disease in mice," by Fitz and colleagues is presented.
- Production of N-Acetylglucosamine Using Recombinant Chitinolytic Enzymes.
Kumar, Sanjeev; Sharma, Rohit; Tewari, Rupinder // Indian Journal of Microbiology; Sep2011, Vol. 51 Issue 3, p319
The pharmaceutically important compound N-acetylglucosamine (NAG), is used in various therapeutic formulations, skin care products and dietary supplements. Currently, NAG is being produced by an environment-unfriendly chemical process using chitin, a polysaccharide present in abundance in the...
- Combined expression of chitinase and lipid transfer protein genes in transgenic carrot plants enhances resistance to foliar fungal pathogens.
J. Jayaraj; Z. Punja // Plant Cell Reports; Sep2007, Vol. 26 Issue 9, p1539
AbstractÃ‚Â Ã‚Â Two pathogenesis-related (PR) protein genes consisting of a barley chitinase (chi-2) and a wheat lipid-transfer-protein (ltp) were introduced singly and in combination into carrot plants via Agrobacterium-mediated transformation using the phosphinothricin acetyl...
- Species relationships between antifungal chitinase and nuclear rDNA (internal transcribed spacer) sequences in the genus Hordeum.
Bustos, Alfredo De; Loarce, Yolanda; Jouve, Nicolás // Genome; Apr2002, Vol. 45 Issue 2, p339
The sequences of the chitinase gene (Chi-26) and the internal transcribed spacer of 18S â€“ 5.8S â€“ 26S rDNA (ITS1) were determined to analyze the phylogenetic relationships among species representing the four basic genomes of the genus Hordeum. Grouping analysis based on data for...
- Transgene stacking and marker elimination in transgenic rice by sequential Agrobacterium-mediated co-transformation with the same selectable marker gene.
Ramana Rao, Mangu Venkata; Parameswari, Chidambaram; Sripriya, Rajasekaran; Veluthambi, Karuppannan // Plant Cell Reports; Jul2011, Vol. 30 Issue 7, p1241
Rice chitinase ( chi11) and tobacco osmotin ( ap24) genes, which cause disruption of fungal cell wall and cell membrane, respectively, were stacked in transgenic rice to develop resistance against the sheath blight disease. The homozygous marker-free transgenic rice line CoT23 which harboured...
- Cloning partial endochitinase cDNA of Trichoderma harzianum antagonistic to Colletotrichum falcatum causing red rot of sugarcane.
Viswanathan, R.; Premkumari, S. Merina; Sundar, A. Ramesh; Kathiresan, T. // Current Science (00113891); 10/10/2006, Vol. 91 Issue 7, p951
The saprophytic fungus, Trichoderma harzianum strain T5 antagonistic to the red rot pathogen of sugarcane, Colletotrichum falcatum excreted chitinases, viz. N-acetyl-Î²-D-glucosaminidase, 1,4-Î²-D-N-N' chitobiosidase and 1,4-Î²-D-N-N'-N" chitotriase into the culture medium containing...
- Confirmation of non-glycan Linked Mannose Glycosylation in Chitanase 24 kDa Secreted by Trichodema harzianum BIO10671.
Muskhazli, M.; Faridah, Q. Z.; Salfarina, R.; Farizan, T. Nor; Nalisha, I.; Wallis, G. L. F. // Asian Journal of Biochemistry; 2008, Vol. 3 Issue 4, p235
Chitinase 42 kDa produced by Trichoderma harzianum has been proven as a prime compound to be excreted onto the hyphae of the pathogen causing localised cell wall lysis at the point of interaction. This finally initiate the process of the host cell becomes empty of cytoplasm, disintegrates and...
- The Characteristics of Chitinase Expression in Aeromonas schubertii.
Chen, Jeen-Kuan; Shen, Chia-Rui; Liu, Chao-Lin // Applied Biochemistry & Biotechnology; Apr2014, Vol. 172 Issue 8, p3827
In this study, chitinase activity in an incubation broth of Aeromonas schubertii was measured using colloidal chitin azure as the substrate. More specifically, the induction of chitinases due to amendment with various carbon sources was examined. The highest chitinase activity was found...
- The Chitinase A from the baculovirus AcMNPV enhances resistance to both fungi and herbivorous pests in tobacco.
Giandomenico Corrado; Stefania Arciello; Paolo Fanti; Luisa Fiandra; Antonio Garonna; Maria Digilio; Matteo Lorito; Barbara Giordana; Francesco Pennacchio; Rosa Rao // Transgenic Research; Aug2008, Vol. 17 Issue 4, p557
AbstractÂ Â Biotechnology has allowed the development of novel strategies to obtain plants that are more resistant to pests, fungal pathogens and other agents of biotic stress. The obvious advantages of having genotypes with multiple beneficial traits have recently fostered the development...
- The effects of the surface-exposed residues on the binding and hydrolytic activities of Vibrio carchariae chitinase A.
Pantoom, Supansa; Songsiriritthigul, Chomphunuch; Suginta, Wipa // BMC Biochemistry; 2008, Vol. 9, Special section p1
Background: Vibrio carchariae chitinase A (EC22.214.171.124) is a family-18 glycosyl hydrolase and comprises three distinct structural domains: i) the amino terminal chitin binding domain (ChBD); ii) the (Î±/Î²)8 TIM barrel catalytic domain (CatD); and iii) the Î± + Î² insertion domain. The...
- Characterization of a chitinolytic enzyme from Serratia sp. KCK isolated from kimchi juice.
Hyun-Soo Kim; Timmis, Kenneth N.; Golyshin, Peter N. // Applied Microbiology & Biotechnology; Jul2007, Vol. 75 Issue 6, p1275
The novel chitinolytic bacterium Serratia sp. KCK, which was isolated from kimchi juice, produced chitinase A. The gene coding for the chitinolytic enzyme was cloned on the basis of sequencing of internal peptides, homology search, and design of degenerated primers. The cloned open reading frame...
- The functional effect of Gly209 and Ile213 substitutions on lysozyme activity of family 19 chitinase encoded by cyanophage Ma-LMM01.
Hosoda, Naohiko; Kurokawa, Yoichi; Sako, Yoshihiko; Nagasaki, Keizo; Yoshida, Takashi; Hiroishi, Shingo // Fisheries Science; Jul2011, Vol. 77 Issue 4, p665
ORF69 in the cyanophage infecting Microcystis aeruginosa, Ma-LMM01, shows homology to the family 19 chitinases where the catalytic domain has structural similarity to lysozyme. Chitinases hydrolyze chitin, a ï¿½-1, 4-linked monopolymer of N-acetylglucosamine (GlcNAc); whereas lysozymes...
- Effect of Carbon Source on Production of Lytic Enzymes by Trichoderma Harzianum.
K���k, �igdem; Kivan�, Merih // Journal of Applied Biological Sciences; May2008, Vol. 2 Issue 2, p23
We investigated the effect of the different carbon sources and phytopathogenic fungal cell walls on production of enzymes by Turkish strain Trichoderma harzianum T15. Trichoderma harzianum T15 secretes ï¿½-1,3-glucanase and chitinase in the presence of different carbon sources. Maximal...
- Chitinase-Mediated Inhibitory Activity of Brassica Transgenic on Growth of Alternaria brassicae.
Mondal, Kalyan K.; Chatterjee, Subhas Chandra; Viswakarma, Navin; Bhattacharya, Ram Charan; Grover, Anita // Current Microbiology; Sep2003, Vol. 47 Issue 3, p0171
Chitinase, capable of degrading the cell walls of invading phytopathogenic fungi, plays an important role in plant defense response, particularly when this enzyme is overexpressed through genetic engineering. In the present study, Brassica plant (Brassica juncea L.) was transformed with...
- Effects of dexamethasone or methotrexate on chitinolytic activity in nasal polyps.
Park, Seong Kook; Hur, Dae Young; Urm, Sang-Hwa // American Journal of Rhinology & Allergy; May/Jun2012, Vol. 26 Issue 3, p183
Background: Chitin is a recognition element for tissue infiltration by innate cells implicated in allergy and immunity. This process can be negatively regulated by vertebrate chitinases. Chitinolytic activity is significantly increased in nasal polyps (NPs) compared with that in normal turbinate...
- Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26.
Essghaier, Badiaa; Rouaissi, Mustapha; Boudabous, Abdellatif; Jijakli, Haissam; Sadfi-Zouaoui, Najla // World Journal of Microbiology & Biotechnology; Jun2010, Vol. 26 Issue 6, p977
This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated from a shallow salt lake in Tunisia. The impact of salt, salinity concentration, pH, carbon and...
- Sequence of the V. parahaemolyticus Gene for Cytoplasmic N,Nâ€²-Diacetylchitobiase and Homology with Related Enzymes1.
Wu, Michael H.; Laine, Roger A. // Journal of Biochemistry; 1999, Vol. 125 Issue 6, p1086
The nucleotide sequence of the gene encoding the cytoplasmic N,Nâ€²-diacetylchitobiase [EC 126.96.36.199] from Vibrio parahaemolyticus (ATCC #27969) has been determined. The deduced peptide sequence of this unusual Î²-hexosaminidase surprisingly shows minimum evolutionary relationship to two...
- Simplified Method of Preparing Colloidal Chitin Used For Screening of Chitinase-Producing Microorganisms.
Murthy, N. K. S.; Bleakley, B. H. // Internet Journal of Microbiology; 2012, Vol. 10 Issue 2, p7
A simplified and efficient method of preparing colloidal chitin from inexpensive crab shell flakes was developed. It modifies some steps of existing techniques to provide significant saving in effort and materials for colloidal chitin preparation. In colloidal chitin preparation, unless the...
- DNA variation in the basic chitinase locus (ChiB) region of the wild plant Arabidopsis thaliana.
Kawabe, Akira; Miyashita, Naohiko T. // Genetics; Nov99, Vol. 153 Issue 3, p1445
Discusses intraspecific nucleotide variation in a 2.2-kbp region of basic chitinase (ChiB) region in Arabidopsis thaliana. Study of genetic mechanisms involved in DNA polymorphism in A. thaliana; Characteristics of the chitinase system as having multiple genes of similar function but distinct...
- ENZYMATIC PROCESSING OF CHITINACEOUS WASTES FOR N-ACETYL-D-GLUCOSAMINE PRODUCTION: AN EXAMPLE OF GREEN AND EFFICIENT ENVIRONMENTAL MANAGEMENT.
Das, Sancharini; Sen, Ramkrishna; Roy, Debasis // Environmental Engineering & Management Journal (EEMJ); Oct2012, Vol. 11 Issue 10, p1849
Chitin from chitinaceous prawn shell waste, fungal biomass of Aspergillus fumigatus and Penicillium waksmanii was used as natural substrate for partially purified chitinase of Trichoderma harzianum to produce N-acetyl-D-glucosamine (GlcNAc), a medically important amino sugar. The isolated...
- Purification and Characterization of an Antifungal Chitinase in Jelly Fig (Ficus awkeotsang) Achenes.
Yu-Ching Li; Chen-Tien Chang; Eric S. L. Hsiao; Joyce S. F. Hsu; Jenn-Wen Huang; Jason T. C. Tzen // Plant & Cell Physiology; Nov2003, Vol. 44 Issue 11, p1162
A method was developed to purify a 30-kDa protein from jelly fig (Ficus awkeotsang) pericarp, including preparation of jelly curd from achenes, extraction of proteins from the curd, and isolation of the 30-kDa protein by anion-exchanger and gel filtration. Chitinase activity was detected in the...
- Effect of Agitation and Aeration Rates on Chitinase Production Using Trichoderma virens UKM1 in 2-l Stirred Tank Reactor.
Christine Fernandez; Madihah Salleh; Rosli Illias; Mohd. Hassan // Applied Biochemistry & Biotechnology; Aug2008, Vol. 150 Issue 2, p193
AbstractÂ Â Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-d-glucosamine (GluNac), a reducing sugar....
- CLONING OF CHITINASE-LIKE PROTEIN1 CDNA FROM DICYEMID MESOZOANS (PHYLUM: DICYEMIDA).
Ogino, Kazutoyo; Tsuneki, Kazuhiko; Furuya, Hidetaka // Journal of Parasitology; Dec2007, Vol. 93 Issue 6, p1403
Dicyemid mesozoans are endoparasites found in the renal sacs of benthic cephalopods. Adult dicyemids insert the distinct anterior region, termed a ''calotte,'' into renal tubules of the host. We cloned cDNA encoding chitinase-like protein from the dicyemid Dicyema japonicum (Dicyema-clp1), and...
- Nutrient regime regulates complex transcriptional start site usage within a Pseudoalteromonas chitinase gene cluster.
Delpin, Marina W.; Goodman, Amanda E. // ISME Journal: Multidisciplinary Journal of Microbial Ecology; Sep2009, Vol. 3 Issue 9, p1053
The chitinase gene cluster of the marine bacterium Pseudoalteromonas sp. S91, chiABC, which produces the major chitinases of this sp., was transcribed as an operon and from each individual gene. chiA, chiB and chiC were found to possess multiple transcriptional start points (TSPs), the use of...
- Mode of action and antifungal properties of two cold-adapted chitinases.
Mavromatis, Konstantinos; Lorito, Matteo; Woo, Sheridan L.; Bouriotis, Vassilis // Extremophiles; Oct2003, Vol. 7 Issue 5, p385
The mode of action of two chitinases from the Antarctic Arthrobacter sp. strain TAD20 on N-acetyl-chitooligomers and chitin polymers has been elucidated. Identification of the length of chitin oligomers following enzymatic hydrolysis was verified by using HPLC-based analysis. It was observed...
- Cloning and characterization of a chitinase gene Lbchi31 from Limonium bicolor and identification of its biological activity.
Zhi Hua Liu; Yu Cheng Wang; Xiao Tian Qi; Chuan Ping Yang // Molecular Biology Reports; Jun2010, Vol. 37 Issue 5, p2447
Chitinases are digestive enzymes that break down glycosidic bonds in chitin. In the current study, an endochitinase gene Lbchi31 was cloned from Limonium bicolor. The cDNA sequence of Lbchi31 was 1,107 bp in length, encoding 322 amino acid residues with a calculated molecular mass of 31.7 kDa....
- Cellular expression of murine Ym1 and Ym2, chitinase family proteins, as revealed by in situ hybridization and immunohistochemistry.
Nio, Junko; Fujimoto, Wakako; Konno, Akihiro; Kon, Yasuhiro; Owhashi, Makoto; Iwanaga, Toshihiko // Histochemistry & Cell Biology; Jun2004, Vol. 121 Issue 6, p473
Ym is one of the chitinase family proteins, which are widely distributed in mammalian bodies and can bind glycosaminoglycans such as heparin/heparan sulfate. Ym1 is a macrophage protein produced in parasitic infections, while its isoform, Ym2, is upregulated in lung under allergic conditions. In...
- Directed evolution for increased chitinase activity.
Yanhua Fan; Weiguo Fang; Yuehua Xiao; Xingyong Yang; Yongjun Zhang; Bidochka, Michael J.; Yan Pei // Applied Microbiology & Biotechnology; Aug2007, Vol. 76 Issue 1, p135
Directed evolution through DNA shuffling and screening was used to enhance the catalytic ability of a fungal, Beauveria bassiana, chitinase, Bbchit1. The Bbchit gene was first linked to various prokaryotic signal sequences and expressed in Escherichia coli. The signal peptide, PelB, from Erwinia...
- A reducing-end-acting chitinase from Vibrio proteolyticus belonging to glycoside hydrolase family 19.
Honda, Yuji; Taniguchi, Hajime; Kitaoka, Motomitsu // Applied Microbiology & Biotechnology; Mar2008, Vol. 78 Issue 4, p627
A chitinase gene belonging to the glycoside hydrolase family 19 from Vibrio proteolyticus ( chi19) was cloned. The recombinant enzyme (Chi19) showed weak activities against polymeric substrates and considerable activities against fully N-acetylated chitooligosaccharides, (GlcNAc) n , whose...
- Comparative Evolutionary Histories of the Fungal Chitinase Gene Family Reveal Non-Random Size Expansions and Contractions due to Adaptive Natural Selection.
Karlsson, Magnus; Stenlid, Jan // Evolutionary Bioinformatics; 2008, Issue 4, p47
Gene duplication and loss play an important role in the evolution of novel functions and for shaping an organism's gene content. Recently, it was suggested that stress-related genes frequently are exposed to duplications and losses, while growth-related genes show selection against change in...
- Role of Tyr-435 of Vibrio harveyi Chitinase A in Chitin Utilization.
Sritho, Natchanok; Suginta, Wipa // Applied Biochemistry & Biotechnology; Apr2012, Vol. 166 Issue 5, p1192
Vibrio harveyi chitinase A or VhChiA (EC.188.8.131.52) is a member of GH-18 chitinases that catalyzes chitin degradation from marine biomaterials. Our earlier structural data of VhChiA suggested that Tyr-435 marks the ending of subsite +2 and may influence binding of the interacting substrate at the...