Citations with the tag: CHITINASE

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  • Purification and some properties of a chitinase isozyme from the liver of Japanese common squid Todarodes pacificus.
    MATSUMIYA, MASAHIRO; MIYAUCHI, KOUJI; MOCHIZUKI, ATSUSHI // Fisheries Science; Apr2003, Vol. 69 Issue 2, p427 

    Describes the purification and properties of chitinase isozyme from the liver of Japanese common squid Todarodes pacificus. Purification steps; Hydrolization of chitin to N-acetylchitooligosaccharides; Chitinase activity; Hydrolysis of chitosan.

  • Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01.
    Xia, Jin-Lan; Xiong, Jing; Zhang, Rui-Yong; Liu, Ke-Ke; Huang, Bin; Nie, Zhen-Yuan // Indian Journal of Microbiology; Sep2011, Vol. 51 Issue 3, p301 

    Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and...

  • Purification, Characterization, and cDNA Cloning of Rice Class III Chitinase.
    Hee-Young Park; Cheol-Ho Pan; Min-Young So; Joong-Hoon Ahn; Do-Hyun Jo; Su-Il Kim // Molecules & Cells; 2002, Vol. 13 Issue 1, p69 

    Presents a study on the expression of chitinases in a rice cell suspension culture. Methodology; Results; Discussion.

  • Erratum: Theor Appl Genet (2001) 103:297–306.
    Hee-Young Park; Cheol-Ho Pan; Min-Young So; Joong-Hoon Ahn; Do-Hyun Jo; Su-Il Kim // Theoretical & Applied Genetics; Dec2001, Vol. 103 Issue 8, p1312 

    Presents a correction to the article "Induced Expression of the Class II Chitinase Gene During Cold Acclimation and Dehydration of Bermudagrass," that was previously published in the periodical "Theory of Applied Genetics."

  • Antibiotics and enzymes produced by the biocontrol agent Streptomyces violaceusniger YCED-9.
    Trejo-Estrada, S R; Paszczynski, A; Crawford, D L // Journal of Industrial Microbiology & Biotechnology; Aug98, Vol. 21 Issue 1/2, p81 

    Streptomyces violaceusniger strain YCED-9 is an antifungal biocontrol agent antagonistic to many different classes of plant pathogenic fungi. We discovered that strain YCED-9 produces three antimicrobial compounds with antifungal activity. These compounds were purified and identified, and...

  • Characterization of 38 kDa and 42 kDa chitinase isozymes from the liver of Japanese common squid Todarodes pacificus.
    MATSUMIYA, Masahiro; MIYAUCHI, Kouji; MOCHIZUKI, Atsushi // Fisheries Science; Jun2002, Vol. 68 Issue 3, p603 

    Characterization was investigated on the 38 kDa and 42 kDa chitinase (EC3.2.1.14) isozymes from the liver of Japanese common squid Todarodes pacificus . Optimum pH toward colloidal chitin was observed at pH 3.0 for the 38 kDa chitinase, and pH 3.0 and 9.0 for the 42 kDa chitinase. K m and k cat...

  • Occurrence and Activity of the Chitinolytic Bacteria of Aeromonas Genus.
    Brzezinska, M. Swiontek; Donderski, W. // Polish Journal of Environmental Studies; 2001, Vol. 10 Issue 1, p27 

    Reports on the findings of a study on the occurrence and activity of chitinolytic planktonic bacteria of Aeromonas genus isolated from lake Jeziorak in Poland. Number of strains decomposing colloidal chitin; Effect of pH influence on chitinases activity; Influence of incubation time on total...

  • Erratum to: Quantification of chitinase and thaumatin-like proteins in grape juices and wines.
    Le Bourse, D.; Conreux, A.; Villaume, S.; Lameiras, P.; Nuzillard, J.-M.; Jeandet, P. // Analytical & Bioanalytical Chemistry; Dec2011, Vol. 401 Issue 5, p1717 

    A correction to the article "Quantification of Chitinase and Thaumatin-Like Proteins in Grape Juices and Wines" that was published in a 2011 issue is presented.

  • A new biomarker in asthma?
    Scott, Ian // Thorax; Apr2008, Vol. 63 Issue 4, p365 

    The article presents a study on the evaluation of the pathogenic role of chitinase-like protein YKL-40 in asthma. Serum YKL-40 levels were measured and investigated in a case-control study which involves three separate cohorts of patients with asthma from Yale, Paris and Wisconsin. It was found...

  • PRODUCTION OF CHITINASE BY STREPTOMYCES HYGROSCOPICUS VMCH2 BY OPTIMISATION OF CULTURAL CONDITIONS.
    PRIYA, C. S.; JAGANNATHAN, N.; KALAICHELVAN, P. T. // International Journal of Pharma & Bio Sciences; Apr-Jun2011, Vol. 2 Issue 2, pB.210 

    A total of 10 chitinolytic bacteria were isolated from 35 soil samples collected from different crop fields in Tamilnadu state, India. Among them, a strain designated as VMCH2 which produced highest chitinolytic activity in primary and secondary screening in colloidal chitin agar was selected...

  • Cell and Molecular Biology, Biochemistry and Molecular Physiology. Three major somatic embryogenesis related proteins in identified as PR proteins.
    Helleboid, Stéphane // Journal of Experimental Botany; Jul2000, Vol. 51 Issue 348 

    Reports on the direct somatic embryogenesis process in leaf tissues of the Cichorium hybrid clone '474' which is accompanied by an overall increase in the amount of proteins secreted into the culture medium. Protein bands found in the conditioned medium; Acidic chitinases present in the...

  • CHI3L1 and CHI3L2 - one protein family, two opposite functions.
    Areshkov, P. O.; Avdieiev, S. S. // Biopolymers & Cell; 2013 Supplement, Vol. 29, p7 

    An abstract of an article about mammalian cells that ectopically produced homologous chitinase-like proteins, CHI3L1 and CHI3L2, is presented and mentions topics including gliomas, cell proliferation, and cell growth.

  • SCREENING OF MARINE VIBRIO SP ISOLATED FROM THE BAYOF BENGAL, INDIA FOR CHITINASE ENZYME PRODUCTION.
    RAVIKUMAR VINAYAGAM; MEIGNANALAKSHMI SUNDARAM // International Journal of Pharma & Bio Sciences; Apr-Jun2013, Vol. 4 Issue 2, pB-238 

    A total of 12 water, sediment and plankton samples were collected from different sampling stations of open sea off the Bay of Bengal coast of Chennai, Tamilnadu, India and 160 isolates of marine Vibrio sp were isolated using Thiosulphate Citrate Bile Sucrose (TCBS) agar medium. The entire...

  • A chitinase with high activity toward partially N-acetylated chitosan from a new, moderately thermophilic, chitin-degrading bacterium, Ralstonia sp. A-471.
    Sutrisno, A.; Ueda, M.; Abe, Y.; Nakazawa, M.; Miyatake, K. // Applied Microbiology & Biotechnology; Jan2004, Vol. 63 Issue 4, p398 

    A moderately thermophilic bacterium, strain A-471, capable of degrading chitin was isolated from a composting system of chitin-containing waste. Analysis of the 16S rDNA sequence revealed that the bacterium belongs to the genus Ralstonia. A thermostable chitinase A (Ra-ChiA) was purified from...

  • Purification, Crystallization and Preliminary Crystallographic Studies of a Class Ii Chitinase from Kidney Bean Seeds.
    Zhaocai Zhou; Hanjian Liu, Z.; Yikun Li; Weimin Gong // Protein & Peptide Letters; Jun2002, Vol. 9 Issue 3, p265 

    A new member of class II chitinase from Phaseolus vulgaris was purified and crystallized. Diffraction data to 2.7� resolution have been collected and the preliminary crystallographic studies have been completed. The space group is P1 with unit cell parameters of a=36.32�,...

  • Production and Purification of Chitinase by Streptomyces sp. from Soil.
    Sowmya, Balakrishnan; Gomathi, Duraisamy; Kalaiselvi, Manokaran; Ravikumar, Ganesan; Arulraj, Chinthamani; Uma, Chandrasekar // Journal of Advanced Scientific Research; Aug2012, Vol. 3 Issue 3, p25 

    Microbial production of chitinase has captured the worldwide attention of both industrial and scientific environments, not only because of its wide spectrum of applications but also for the lacuna of an effective production method. Chitinase production by a terrestrial Streptomyces species was...

  • Thermodynamics of Chitinase Partitioning in Soy Lecithin Liposomes and Their Storage Stability.
    Cano-Salazar, Luc�a; Ju�rez-Ord�z, Antonio; Gregorio-J�uregui, Karla; Mart�nez-Hern�ndez, Jos�; Rodr�guez-Mart�nez, Jes�s; Ilyina, Anna // Applied Biochemistry & Biotechnology; Dec2011, Vol. 165 Issue 7/8, p1611 

    The goal of this study was to define the partitioning behavior of chitinase from Trichoderma spp. in soy lecithin liposomes, using a thermodynamic approach based on the partitioning variation with temperature. An effort has been made to define the liposomes, as well as free and immobilized...

  • Acidic mammalian chitinase is not a critical target for allergic airway disease in mice.
    Fitz, L. J.; DeClercq, C.; Brooks, J.; Kuang, W.; Bates, B.; Demers, D.; Winkler, A.; Nocka, K.; Jiao, A.; Greco, R. M.; Mason, L. E.; Fleming, M.; Quazi, A.; Wright, J.; Goldman, S.; Hubeau, C.; Williams, C. M. // Journal of Inflammation;  

    An abstract of the article "Acidic mammalian chitinase is not a critical target for allergic airway disease in mice," by Fitz and colleagues is presented.

  • Production of N-Acetylglucosamine Using Recombinant Chitinolytic Enzymes.
    Kumar, Sanjeev; Sharma, Rohit; Tewari, Rupinder // Indian Journal of Microbiology; Sep2011, Vol. 51 Issue 3, p319 

    The pharmaceutically important compound N-acetylglucosamine (NAG), is used in various therapeutic formulations, skin care products and dietary supplements. Currently, NAG is being produced by an environment-unfriendly chemical process using chitin, a polysaccharide present in abundance in the...

  • Combined expression of chitinase and lipid transfer protein genes in transgenic carrot plants enhances resistance to foliar fungal pathogens.
    J. Jayaraj; Z. Punja // Plant Cell Reports; Sep2007, Vol. 26 Issue 9, p1539 

    Abstract  Two pathogenesis-related (PR) protein genes consisting of a barley chitinase (chi-2) and a wheat lipid-transfer-protein (ltp) were introduced singly and in combination into carrot plants via Agrobacterium-mediated transformation using the phosphinothricin acetyl...

  • Species relationships between antifungal chitinase and nuclear rDNA (internal transcribed spacer) sequences in the genus Hordeum.
    Bustos, Alfredo De; Loarce, Yolanda; Jouve, Nicolás // Genome; Apr2002, Vol. 45 Issue 2, p339 

    The sequences of the chitinase gene (Chi-26) and the internal transcribed spacer of 18S – 5.8S – 26S rDNA (ITS1) were determined to analyze the phylogenetic relationships among species representing the four basic genomes of the genus Hordeum. Grouping analysis based on data for...

  • Transgene stacking and marker elimination in transgenic rice by sequential Agrobacterium-mediated co-transformation with the same selectable marker gene.
    Ramana Rao, Mangu Venkata; Parameswari, Chidambaram; Sripriya, Rajasekaran; Veluthambi, Karuppannan // Plant Cell Reports; Jul2011, Vol. 30 Issue 7, p1241 

    Rice chitinase ( chi11) and tobacco osmotin ( ap24) genes, which cause disruption of fungal cell wall and cell membrane, respectively, were stacked in transgenic rice to develop resistance against the sheath blight disease. The homozygous marker-free transgenic rice line CoT23 which harboured...

  • Confirmation of non-glycan Linked Mannose Glycosylation in Chitanase 24 kDa Secreted by Trichodema harzianum BIO10671.
    Muskhazli, M.; Faridah, Q. Z.; Salfarina, R.; Farizan, T. Nor; Nalisha, I.; Wallis, G. L. F. // Asian Journal of Biochemistry; 2008, Vol. 3 Issue 4, p235 

    Chitinase 42 kDa produced by Trichoderma harzianum has been proven as a prime compound to be excreted onto the hyphae of the pathogen causing localised cell wall lysis at the point of interaction. This finally initiate the process of the host cell becomes empty of cytoplasm, disintegrates and...

  • The Chitinase A from the baculovirus AcMNPV enhances resistance to both fungi and herbivorous pests in tobacco.
    Giandomenico Corrado; Stefania Arciello; Paolo Fanti; Luisa Fiandra; Antonio Garonna; Maria Digilio; Matteo Lorito; Barbara Giordana; Francesco Pennacchio; Rosa Rao // Transgenic Research; Aug2008, Vol. 17 Issue 4, p557 

    Abstract  Biotechnology has allowed the development of novel strategies to obtain plants that are more resistant to pests, fungal pathogens and other agents of biotic stress. The obvious advantages of having genotypes with multiple beneficial traits have recently fostered the development...

  • Serum YKL-40 and colorectal cancer.
    Cintin, C; Johansen, J S; Christensen, I J; Price, P A; Sørensen, S; Nielsen, H J // British Journal of Cancer; 3/15/99, Vol. 79 Issue 9/10, p1494 

    YKL-40 is a mammalian member of the chitinase protein family. Although the function of YKL-40 is unknown, the pattern of its expression suggests a function in remodelling or degradation of extracellular matrix. High serum YKL-40 has been found in patients with recurrent breast cancer and has...

  • Detection and characterization of chitinases and other chitin-modifying enzymes.
    Howard, Michael B.; Ekborg, Nathan A.; Weiner, Ronald M.; Hutcheson, Steven W. // Journal of Industrial Microbiology & Biotechnology; Nov2003, Vol. 30 Issue 11, p627 

    Multiple industrial and medical uses of chitin and its derivatives have been developed in recent years. The demand for enzymes with new or desirable properties continues to grow as additional uses of chitin, chitooligosaccharides, and chitosan become apparent. Microorganisms, the primary...

  • Cloning partial endochitinase cDNA of Trichoderma harzianum antagonistic to Colletotrichum falcatum causing red rot of sugarcane.
    Viswanathan, R.; Premkumari, S. Merina; Sundar, A. Ramesh; Kathiresan, T. // Current Science (00113891); 10/10/2006, Vol. 91 Issue 7, p951 

    The saprophytic fungus, Trichoderma harzianum strain T5 antagonistic to the red rot pathogen of sugarcane, Colletotrichum falcatum excreted chitinases, viz. N-acetyl-β-D-glucosaminidase, 1,4-β-D-N-N' chitobiosidase and 1,4-β-D-N-N'-N" chitotriase into the culture medium containing...

  • Response of transgenic cucumber expressing a rice class I chitinase gene to two fungal pathogens with different infectivities.
    Kishimoto, Kyutaro; Nakajima, Masami; Nishizawa, Yoko; Tabei, Yutaka; Hibi, Tadaaki; Akutsu, Katsumi // Journal of General Plant Pathology; Dec2003, Vol. 69 Issue 6, p358 

    A transgenic cucumber line (CR32) over-expressing the rice class I chitinase gene exhibited resistance to Phytophthora rot (Phytophthora nicotianae var. parasitica) but not to Fusarium wilt (Fusarium oxysporum f. sp. cucumerinum). The infection behavior of these fungi on CR32 and nontransgenic...

  • The Characteristics of Chitinase Expression in Aeromonas schubertii.
    Chen, Jeen-Kuan; Shen, Chia-Rui; Liu, Chao-Lin // Applied Biochemistry & Biotechnology; Apr2014, Vol. 172 Issue 8, p3827 

    In this study, chitinase activity in an incubation broth of Aeromonas schubertii was measured using colloidal chitin azure as the substrate. More specifically, the induction of chitinases due to amendment with various carbon sources was examined. The highest chitinase activity was found...

  • The effects of the surface-exposed residues on the binding and hydrolytic activities of Vibrio carchariae chitinase A.
    Pantoom, Supansa; Songsiriritthigul, Chomphunuch; Suginta, Wipa // BMC Biochemistry; 2008, Vol. 9, Special section p1 

    Background: Vibrio carchariae chitinase A (EC3.2.1.14) is a family-18 glycosyl hydrolase and comprises three distinct structural domains: i) the amino terminal chitin binding domain (ChBD); ii) the (α/β)8 TIM barrel catalytic domain (CatD); and iii) the α + β insertion domain. The...

  • Biotechnological aspects of chitinolytic enzymes: a review.
    Dahiya, Neetu; Tewari, Rupinder; Hoondal, Gurinder Singh // Applied Microbiology & Biotechnology; Aug2006, Vol. 71 Issue 6, p773 

    Chitin and chitinases (EC 3.2.1.14) have an immense potential. Chitinolytic enzymes have wide-ranging applications such as preparation of pharmaceutically important chitooligosaccharides and N-acetyl d-glucosamine, preparation of single-cell protein, isolation of protoplasts from fungi and...

  • Optimization of nutrients for chitinase production by Serratia marcescens JPP1 against aflatoxin using statistical experimental design.
    Kai Wang; Pei sheng Yan; Li xin Cao // Journal of Chemical & Pharmaceutical Research; 2013, Vol. 5 Issue 12, p447 

    Chitinase is produced by a number of organisms and it is one of the most important enzymes with industrial significance. In this study, we describe the optimization of medium composition with increased production of chitinase for Serratia marcescens strain JPP1. The strain was isolated from the...

  • Characterization of a chitinolytic enzyme from Serratia sp. KCK isolated from kimchi juice.
    Hyun-Soo Kim; Timmis, Kenneth N.; Golyshin, Peter N. // Applied Microbiology & Biotechnology; Jul2007, Vol. 75 Issue 6, p1275 

    The novel chitinolytic bacterium Serratia sp. KCK, which was isolated from kimchi juice, produced chitinase A. The gene coding for the chitinolytic enzyme was cloned on the basis of sequencing of internal peptides, homology search, and design of degenerated primers. The cloned open reading frame...

  • Effect of Carbon Source on Production of Lytic Enzymes by Trichoderma Harzianum.
    K���k, �igdem; Kivan�, Merih // Journal of Applied Biological Sciences; May2008, Vol. 2 Issue 2, p23 

    We investigated the effect of the different carbon sources and phytopathogenic fungal cell walls on production of enzymes by Turkish strain Trichoderma harzianum T15. Trichoderma harzianum T15 secretes �-1,3-glucanase and chitinase in the presence of different carbon sources. Maximal...

  • Chitinase-Mediated Inhibitory Activity of Brassica Transgenic on Growth of Alternaria brassicae.
    Mondal, Kalyan K.; Chatterjee, Subhas Chandra; Viswakarma, Navin; Bhattacharya, Ram Charan; Grover, Anita // Current Microbiology; Sep2003, Vol. 47 Issue 3, p0171 

    Chitinase, capable of degrading the cell walls of invading phytopathogenic fungi, plays an important role in plant defense response, particularly when this enzyme is overexpressed through genetic engineering. In the present study, Brassica plant (Brassica juncea L.) was transformed with...

  • Effects of dexamethasone or methotrexate on chitinolytic activity in nasal polyps.
    Park, Seong Kook; Hur, Dae Young; Urm, Sang-Hwa // American Journal of Rhinology & Allergy; May/Jun2012, Vol. 26 Issue 3, p183 

    Background: Chitin is a recognition element for tissue infiltration by innate cells implicated in allergy and immunity. This process can be negatively regulated by vertebrate chitinases. Chitinolytic activity is significantly increased in nasal polyps (NPs) compared with that in normal turbinate...

  • Simplified Method of Preparing Colloidal Chitin Used For Screening of Chitinase-Producing Microorganisms.
    Murthy, N. K. S.; Bleakley, B. H. // Internet Journal of Microbiology; 2012, Vol. 10 Issue 2, p7 

    A simplified and efficient method of preparing colloidal chitin from inexpensive crab shell flakes was developed. It modifies some steps of existing techniques to provide significant saving in effort and materials for colloidal chitin preparation. In colloidal chitin preparation, unless the...

  • ENZYMATIC PROCESSING OF CHITINACEOUS WASTES FOR N-ACETYL-D-GLUCOSAMINE PRODUCTION: AN EXAMPLE OF GREEN AND EFFICIENT ENVIRONMENTAL MANAGEMENT.
    Das, Sancharini; Sen, Ramkrishna; Roy, Debasis // Environmental Engineering & Management Journal (EEMJ); Oct2012, Vol. 11 Issue 10, p1849 

    Chitin from chitinaceous prawn shell waste, fungal biomass of Aspergillus fumigatus and Penicillium waksmanii was used as natural substrate for partially purified chitinase of Trichoderma harzianum to produce N-acetyl-D-glucosamine (GlcNAc), a medically important amino sugar. The isolated...

  • Effect of Agitation and Aeration Rates on Chitinase Production Using Trichoderma virens UKM1 in 2-l Stirred Tank Reactor.
    Christine Fernandez; Madihah Salleh; Rosli Illias; Mohd. Hassan // Applied Biochemistry & Biotechnology; Aug2008, Vol. 150 Issue 2, p193 

    Abstract  Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-d-glucosamine (GluNac), a reducing sugar....

  • Mode of action and antifungal properties of two cold-adapted chitinases.
    Mavromatis, Konstantinos; Lorito, Matteo; Woo, Sheridan L.; Bouriotis, Vassilis // Extremophiles; Oct2003, Vol. 7 Issue 5, p385 

    The mode of action of two chitinases from the Antarctic Arthrobacter sp. strain TAD20 on N-acetyl-chitooligomers and chitin polymers has been elucidated. Identification of the length of chitin oligomers following enzymatic hydrolysis was verified by using HPLC-based analysis. It was observed...

  • Cellular expression of murine Ym1 and Ym2, chitinase family proteins, as revealed by in situ hybridization and immunohistochemistry.
    Nio, Junko; Fujimoto, Wakako; Konno, Akihiro; Kon, Yasuhiro; Owhashi, Makoto; Iwanaga, Toshihiko // Histochemistry & Cell Biology; Jun2004, Vol. 121 Issue 6, p473 

    Ym is one of the chitinase family proteins, which are widely distributed in mammalian bodies and can bind glycosaminoglycans such as heparin/heparan sulfate. Ym1 is a macrophage protein produced in parasitic infections, while its isoform, Ym2, is upregulated in lung under allergic conditions. In...

  • Comparative Evolutionary Histories of the Fungal Chitinase Gene Family Reveal Non-Random Size Expansions and Contractions due to Adaptive Natural Selection.
    Karlsson, Magnus; Stenlid, Jan // Evolutionary Bioinformatics; 2008, Issue 4, p47 

    Gene duplication and loss play an important role in the evolution of novel functions and for shaping an organism's gene content. Recently, it was suggested that stress-related genes frequently are exposed to duplications and losses, while growth-related genes show selection against change in...

  • Role of Tyr-435 of Vibrio harveyi Chitinase A in Chitin Utilization.
    Sritho, Natchanok; Suginta, Wipa // Applied Biochemistry & Biotechnology; Apr2012, Vol. 166 Issue 5, p1192 

    Vibrio harveyi chitinase A or VhChiA (EC.3.2.1.14) is a member of GH-18 chitinases that catalyzes chitin degradation from marine biomaterials. Our earlier structural data of VhChiA suggested that Tyr-435 marks the ending of subsite +2 and may influence binding of the interacting substrate at the...

  • Purification and Characterisation of a 31-kDa Chitinase from the Myzus Persicae Aphid: A Target for Hemiptera Biocontrol.
    Francis, Frédéric; Saguez, Julien; Cherqui, Anas; Vandermoten, Sophie; Vincent, Charles; Versali, Marie-France; Dommès, Jacques; Pauw, Edwin; Giordanengo, Philippe; Haubruge, Eric // Applied Biochemistry & Biotechnology; Apr2012, Vol. 166 Issue 5, p1291 

    Hydrolytic enzymes involved in chitin degradation are important to allow moulting during insect development. Chitinases are interesting targets to disturb growth and develop alternative strategies to control insect pests. In this work, a chitinase from the aphid Myzus persicae was purified with...

  • A plant class V chitinase from a cycad (Cycas revoluta): Biochemical characterization, cDNA isolation, and posttranslational modification.
    Taira, Toki; Hayashi, Hiroko; Tajiri, Yoshiko; Onaga, Shoko; Uechi, Gen-ichiro; Iwasaki, Hironori; Ohnuma, Takayuki; Fukamizo, Tamo // Glycobiology; Dec2009, Vol. 19 Issue 12, p1452 

    Chitinase-A (CrChi-A) was purified from leaf rachises of Cycas revoluta by several steps of column chromatography. It was found to be a glycoprotein with a molecular mass of 40 kDa and an isoelectric point of 5.6. CrChi-A produced mainly (GlcNAc)3 from the substrate (GlcNAc)6 through a retaining...

  • A small chimerically bifunctional monomeric protein: Tapes japonica lysozyme.
    Takeshita, K.; Hashimoto, Y.; Ueda, T.; Imoto, T. // Cellular & Molecular Life Sciences; Sep2003, Vol. 60 Issue 9, p1944 

    The lysozyme of the marine bilave Tapes japonica (13.8 kDa) is a novel protein. The protein has 46% homology with the destabilase from medicinal leech that has isopeptidase activity. Based on these data, we confirmed hydrolysis activity of T. japonica lysozyme against three substrates:...

  • Evolution, Homology Conservation, and Identification of Unique Sequence Signatures in GH19 Family Chitinases.
    Udaya Prakash, N. A.; Jayanthi, M.; Sabarinathan, R.; Kangueane, P.; Mathew, Lazar; Sekar, K. // Journal of Molecular Evolution; May2010, Vol. 70 Issue 5, p466 

    The discovery of GH (Glycoside Hydrolase) 19 chitinases in Streptomyces sp. raises the possibility of the presence of these proteins in other bacterial species, since they were initially thought to be confined to higher plants. The present study mainly concentrates on the phylogenetic...

  • Distribution of Chitinases in the Entomopathogen Metarhizium anisopliae and Effect of N-Acetylglucosamine in Protein Secretion.
    Barreto, Cristine Chaves; Staats, Charley Christian; Schrank, Augusto; Vainstein, Marilene Henning // Current Microbiology; Feb2004, Vol. 48 Issue 2, p102 

    For a long time, fungi have been characterized by their ability to secrete enzymes, mostly hydrolytic in function, and thus are defined as extracellular degraders. Chitin and chitinolytic enzymes are gaining importance for their biotechnological applications. Particularly, chitinases are used in...

  • STUDIES ON SOIL WITH RESPECT TO CHITINOLYTIC BACILLUS FROM AURANGABAD AND AKOLA DISTRICT (M.S.) INDIA.
    Khan, Rehan Saleem; Khan, Zia H. // Bioscience Discovery: An International Journal of Life Sciences; Jul2011, Vol. 2 Issue 3, p328 

    The cheapest source of isolation of bacillus is soil so it becomes necessary to know that which soil having more number of chitinolytic bacillus hence present comparative study was done on the soil from Aurangabad and Akola district. The method for isolation of chitinolytic Bacillus depends on...

  • Optimization of Cultural Conditions for Production of Chitinase by a Soil Isolate of Massilia timonae.
    Faramarzi, M.A.; Fazeli, M.; Yazdi, M. Tabatabaei; Adrangi, S.; Al Ahmadi, K. Jami; Tasharrofi, N.; Mohseni, F. Aziz // Biotechnology; 2009, Vol. 8 Issue 1, p93 

    The aim of this study was to characterize chitinase-producing bacteria isolated from environmental samples and to investigate conditions affecting chitinase production by these bacteria. Ninety-eight isolates recovered from 20 soil samples were screened for chitinase production. Eighteen...

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