Citations with the tag: CHITINASE
Results 1 - 50
- Occurrence and Activity of the Chitinolytic Bacteria of Aeromonas Genus.
Brzezinska, M. Swiontek; Donderski, W. // Polish Journal of Environmental Studies; 2001, Vol. 10 Issue 1, p27
Reports on the findings of a study on the occurrence and activity of chitinolytic planktonic bacteria of Aeromonas genus isolated from lake Jeziorak in Poland. Number of strains decomposing colloidal chitin; Effect of pH influence on chitinases activity; Influence of incubation time on total...
- Antibiotics and enzymes produced by the biocontrol agent Streptomyces violaceusniger YCED-9.
Trejo-Estrada, S R; Paszczynski, A; Crawford, D L // Journal of Industrial Microbiology & Biotechnology; Aug98, Vol. 21 Issue 1/2, p81
Streptomyces violaceusniger strain YCED-9 is an antifungal biocontrol agent antagonistic to many different classes of plant pathogenic fungi. We discovered that strain YCED-9 produces three antimicrobial compounds with antifungal activity. These compounds were purified and identified, and...
- Purification and some properties of a chitinase isozyme from the liver of Japanese common squid Todarodes pacificus.
MATSUMIYA, MASAHIRO; MIYAUCHI, KOUJI; MOCHIZUKI, ATSUSHI // Fisheries Science; Apr2003, Vol. 69 Issue 2, p427
Describes the purification and properties of chitinase isozyme from the liver of Japanese common squid Todarodes pacificus. Purification steps; Hydrolization of chitin to N-acetylchitooligosaccharides; Chitinase activity; Hydrolysis of chitosan.
- Cell and Molecular Biology, Biochemistry and Molecular Physiology. Three major somatic embryogenesis related proteins in identified as PR proteins.
Helleboid, St�phane // Journal of Experimental Botany; Jul2000, Vol. 51 Issue 348
Reports on the direct somatic embryogenesis process in leaf tissues of the Cichorium hybrid clone '474' which is accompanied by an overall increase in the amount of proteins secreted into the culture medium. Protein bands found in the conditioned medium; Acidic chitinases present in the...
- Erratum to: Quantification of chitinase and thaumatin-like proteins in grape juices and wines.
Le Bourse, D.; Conreux, A.; Villaume, S.; Lameiras, P.; Nuzillard, J.-M.; Jeandet, P. // Analytical & Bioanalytical Chemistry; Dec2011, Vol. 401 Issue 5, p1717
A correction to the article "Quantification of Chitinase and Thaumatin-Like Proteins in Grape Juices and Wines" that was published in a 2011 issue is presented.
- A new biomarker in asthma?
Scott, Ian // Thorax; Apr2008, Vol. 63 Issue 4, p365
The article presents a study on the evaluation of the pathogenic role of chitinase-like protein YKL-40 in asthma. Serum YKL-40 levels were measured and investigated in a case-control study which involves three separate cohorts of patients with asthma from Yale, Paris and Wisconsin. It was found...
- Production of Chitinase and its Optimization from a Novel Isolate Serratia marcescens XJ-01.
Xia, Jin-Lan; Xiong, Jing; Zhang, Rui-Yong; Liu, Ke-Ke; Huang, Bin; Nie, Zhen-Yuan // Indian Journal of Microbiology; Sep2011, Vol. 51 Issue 3, p301
Production of chitinase from bacteria has distinct advantages over fungi, due to the formation of mycelia of fungi in the later phase of fermentation. A novel chitinase-producing bacterial strain XJ-01 was isolated from the Yulu fishing field of Changsha, Hunan province, China, by enrichment and...
- Characterization of 38 kDa and 42 kDa chitinase isozymes from the liver of Japanese common squid Todarodes pacificus.
MATSUMIYA, Masahiro; MIYAUCHI, Kouji; MOCHIZUKI, Atsushi // Fisheries Science; Jun2002, Vol. 68 Issue 3, p603
Characterization was investigated on the 38 kDa and 42 kDa chitinase (EC22.214.171.124) isozymes from the liver of Japanese common squid Todarodes pacificus . Optimum pH toward colloidal chitin was observed at pH 3.0 for the 38 kDa chitinase, and pH 3.0 and 9.0 for the 42 kDa chitinase. K m and k cat...
- Purification, Characterization, and cDNA Cloning of Rice Class III Chitinase.
Hee-Young Park; Cheol-Ho Pan; Min-Young So; Joong-Hoon Ahn; Do-Hyun Jo; Su-Il Kim // Molecules & Cells; 2002, Vol. 13 Issue 1, p69
Presents a study on the expression of chitinases in a rice cell suspension culture. Methodology; Results; Discussion.
- PRODUCTION OF CHITINASE BY STREPTOMYCES HYGROSCOPICUS VMCH2 BY OPTIMISATION OF CULTURAL CONDITIONS.
PRIYA, C. S.; JAGANNATHAN, N.; KALAICHELVAN, P. T. // International Journal of Pharma & Bio Sciences; Apr-Jun2011, Vol. 2 Issue 2, pB.210
A total of 10 chitinolytic bacteria were isolated from 35 soil samples collected from different crop fields in Tamilnadu state, India. Among them, a strain designated as VMCH2 which produced highest chitinolytic activity in primary and secondary screening in colloidal chitin agar was selected...
- SCREENING OF MARINE VIBRIO SP ISOLATED FROM THE BAYOF BENGAL, INDIA FOR CHITINASE ENZYME PRODUCTION.
RAVIKUMAR VINAYAGAM; MEIGNANALAKSHMI SUNDARAM // International Journal of Pharma & Bio Sciences; Apr-Jun2013, Vol. 4 Issue 2, pB-238
A total of 12 water, sediment and plankton samples were collected from different sampling stations of open sea off the Bay of Bengal coast of Chennai, Tamilnadu, India and 160 isolates of marine Vibrio sp were isolated using Thiosulphate Citrate Bile Sucrose (TCBS) agar medium. The entire...
- Erratum: Theor Appl Genet (2001) 103:297â€“306.
RAVIKUMAR VINAYAGAM; MEIGNANALAKSHMI SUNDARAM // Theoretical & Applied Genetics; Dec2001, Vol. 103 Issue 8, p1312
Presents a correction to the article "Induced Expression of the Class II Chitinase Gene During Cold Acclimation and Dehydration of Bermudagrass," that was previously published in the periodical "Theory of Applied Genetics."
- Detection and characterization of chitinases and other chitin-modifying enzymes.
Howard, Michael B.; Ekborg, Nathan A.; Weiner, Ronald M.; Hutcheson, Steven W. // Journal of Industrial Microbiology & Biotechnology; Nov2003, Vol. 30 Issue 11, p627
Multiple industrial and medical uses of chitin and its derivatives have been developed in recent years. The demand for enzymes with new or desirable properties continues to grow as additional uses of chitin, chitooligosaccharides, and chitosan become apparent. Microorganisms, the primary...
- A chitinase with high activity toward partially N-acetylated chitosan from a new, moderately thermophilic, chitin-degrading bacterium, Ralstonia sp. A-471.
Sutrisno, A.; Ueda, M.; Abe, Y.; Nakazawa, M.; Miyatake, K. // Applied Microbiology & Biotechnology; Jan2004, Vol. 63 Issue 4, p398
A moderately thermophilic bacterium, strain A-471, capable of degrading chitin was isolated from a composting system of chitin-containing waste. Analysis of the 16S rDNA sequence revealed that the bacterium belongs to the genus Ralstonia. A thermostable chitinase A (Ra-ChiA) was purified from...
- Biotechnological aspects of chitinolytic enzymes: a review.
Dahiya, Neetu; Tewari, Rupinder; Hoondal, Gurinder Singh // Applied Microbiology & Biotechnology; Aug2006, Vol. 71 Issue 6, p773
Chitin and chitinases (EC 126.96.36.199) have an immense potential. Chitinolytic enzymes have wide-ranging applications such as preparation of pharmaceutically important chitooligosaccharides and N-acetyl d-glucosamine, preparation of single-cell protein, isolation of protoplasts from fungi and...
- Purification, Crystallization and Preliminary Crystallographic Studies of a Class Ii Chitinase from Kidney Bean Seeds.
Zhaocai Zhou; Hanjian Liu, Z.; Yikun Li; Weimin Gong // Protein & Peptide Letters; Jun2002, Vol. 9 Issue 3, p265
A new member of class II chitinase from Phaseolus vulgaris was purified and crystallized. Diffraction data to 2.7ï¿½ resolution have been collected and the preliminary crystallographic studies have been completed. The space group is P1 with unit cell parameters of a=36.32ï¿½,...
- Response of transgenic cucumber expressing a rice class I chitinase gene to two fungal pathogens with different infectivities.
Kishimoto, Kyutaro; Nakajima, Masami; Nishizawa, Yoko; Tabei, Yutaka; Hibi, Tadaaki; Akutsu, Katsumi // Journal of General Plant Pathology; Dec2003, Vol. 69 Issue 6, p358
A transgenic cucumber line (CR32) over-expressing the rice class I chitinase gene exhibited resistance to Phytophthora rot (Phytophthora nicotianae var. parasitica) but not to Fusarium wilt (Fusarium oxysporum f. sp. cucumerinum). The infection behavior of these fungi on CR32 and nontransgenic...
- Production and Purification of Chitinase by Streptomyces sp. from Soil.
Sowmya, Balakrishnan; Gomathi, Duraisamy; Kalaiselvi, Manokaran; Ravikumar, Ganesan; Arulraj, Chinthamani; Uma, Chandrasekar // Journal of Advanced Scientific Research; Aug2012, Vol. 3 Issue 3, p25
Microbial production of chitinase has captured the worldwide attention of both industrial and scientific environments, not only because of its wide spectrum of applications but also for the lacuna of an effective production method. Chitinase production by a terrestrial Streptomyces species was...
- Thermodynamics of Chitinase Partitioning in Soy Lecithin Liposomes and Their Storage Stability.
Cano-Salazar, Luc�a; Ju�rez-Ord�z, Antonio; Gregorio-J�uregui, Karla; Mart�nez-Hern�ndez, Jos�; Rodr�guez-Mart�nez, Jes�s; Ilyina, Anna // Applied Biochemistry & Biotechnology; Dec2011, Vol. 165 Issue 7/8, p1611
The goal of this study was to define the partitioning behavior of chitinase from Trichoderma spp. in soy lecithin liposomes, using a thermodynamic approach based on the partitioning variation with temperature. An effort has been made to define the liposomes, as well as free and immobilized...
- Acidic mammalian chitinase is not a critical target for allergic airway disease in mice.
Fitz, L. J.; DeClercq, C.; Brooks, J.; Kuang, W.; Bates, B.; Demers, D.; Winkler, A.; Nocka, K.; Jiao, A.; Greco, R. M.; Mason, L. E.; Fleming, M.; Quazi, A.; Wright, J.; Goldman, S.; Hubeau, C.; Williams, C. M. // Journal of Inflammation;
An abstract of the article "Acidic mammalian chitinase is not a critical target for allergic airway disease in mice," by Fitz and colleagues is presented.
- Cloning partial endochitinase cDNA of Trichoderma harzianum antagonistic to Colletotrichum falcatum causing red rot of sugarcane.
Viswanathan, R.; Premkumari, S. Merina; Sundar, A. Ramesh; Kathiresan, T. // Current Science (00113891); 10/10/2006, Vol. 91 Issue 7, p951
The saprophytic fungus, Trichoderma harzianum strain T5 antagonistic to the red rot pathogen of sugarcane, Colletotrichum falcatum excreted chitinases, viz. N-acetyl-Î²-D-glucosaminidase, 1,4-Î²-D-N-N' chitobiosidase and 1,4-Î²-D-N-N'-N" chitotriase into the culture medium containing...
- Production of N-Acetylglucosamine Using Recombinant Chitinolytic Enzymes.
Kumar, Sanjeev; Sharma, Rohit; Tewari, Rupinder // Indian Journal of Microbiology; Sep2011, Vol. 51 Issue 3, p319
The pharmaceutically important compound N-acetylglucosamine (NAG), is used in various therapeutic formulations, skin care products and dietary supplements. Currently, NAG is being produced by an environment-unfriendly chemical process using chitin, a polysaccharide present in abundance in the...
- Combined expression of chitinase and lipid transfer protein genes in transgenic carrot plants enhances resistance to foliar fungal pathogens.
J. Jayaraj; Z. Punja // Plant Cell Reports; Sep2007, Vol. 26 Issue 9, p1539
AbstractÃ‚Â Ã‚Â Two pathogenesis-related (PR) protein genes consisting of a barley chitinase (chi-2) and a wheat lipid-transfer-protein (ltp) were introduced singly and in combination into carrot plants via Agrobacterium-mediated transformation using the phosphinothricin acetyl...
- Species relationships between antifungal chitinase and nuclear rDNA (internal transcribed spacer) sequences in the genus Hordeum.
Bustos, Alfredo De; Loarce, Yolanda; Jouve, Nicolás // Genome; Apr2002, Vol. 45 Issue 2, p339
The sequences of the chitinase gene (Chi-26) and the internal transcribed spacer of 18S â€“ 5.8S â€“ 26S rDNA (ITS1) were determined to analyze the phylogenetic relationships among species representing the four basic genomes of the genus Hordeum. Grouping analysis based on data for...
- Transgene stacking and marker elimination in transgenic rice by sequential Agrobacterium-mediated co-transformation with the same selectable marker gene.
Ramana Rao, Mangu Venkata; Parameswari, Chidambaram; Sripriya, Rajasekaran; Veluthambi, Karuppannan // Plant Cell Reports; Jul2011, Vol. 30 Issue 7, p1241
Rice chitinase ( chi11) and tobacco osmotin ( ap24) genes, which cause disruption of fungal cell wall and cell membrane, respectively, were stacked in transgenic rice to develop resistance against the sheath blight disease. The homozygous marker-free transgenic rice line CoT23 which harboured...
- Cloning and sequencing of two genes encoding chitinases A and B from Bacillus cereus CH.
Mabuchi, Naoto; Araki, Yoshio // Canadian Journal of Microbiology; Oct2001, Vol. 47 Issue 10, p895
Two genes encoding chitinases A and B (chiA and chiB) from Bacillus cereus CH were cloned into Escherichia coli XL1-Blue MRF' by using pBluescript II SK[sup +] , and their nucleotide sequences were determined. Open reading frames of the chiA and chiB genes encoded distinct polypeptide chains...
- Isolation and characterization of chitinases from Verticillium lecanii.
Zhen-Xiang Lu; Laroche, Andr�; Hung Chang Huang // Canadian Journal of Microbiology; Dec2005, Vol. 51 Issue 12, p1045
Degenerate PCR primers corresponding to conserved domains of fungal chitinases were designed, and PCR was performed on genomic DNA of the entomogenous fungus Verticillium lecanii (Zimmermann) Viegas. Two distinct PCR fragments, chf1 and chf2, were isolated and used to identify two DNA contigs....
- Characterization of a chitinase and an endo-ï¿½-1,3-glucanase from Trichoderma harzianum Rifai T24 involved in control of the phytopathogen Sclerotium rolfsii.
El-Katatny, M. H.; Gudelj, M.; Robra, K.-H.; Elnaghy, M. A.; G�bitz, G. M. // Applied Microbiology & Biotechnology; Jul2001, Vol. 56 Issue 1/2, p137
Of 24 Trichoderma isolates, T. harzianum Rifai (T24) showed a potential for control of the phytopathogenic basidiomycete Sclerotium rolfsii. When T24 was grown on different carbon sources, growth inhibition of S. rolfsii by the T24 culture filtrate correlated with the activity of extracellular...
- Expression of the chitinase gene from Trichoderma aureoviride in Saccharomyces cerevisiae.
Song Jinzhu; Yang Qian; Liu Beidong; Chen Dianfu // Applied Microbiology & Biotechnology; Nov2005, Vol. 69 Issue 1, p39
Chitinase gene ech42 was obtained from Trichoderma aureoviride M and amplified by PCR. The isolated DNA of ech42 was then sequenced. The results showed that the open reading frame of ech42 was 1,447 bp long, encoding 421 amino acids. Three introns were found in the sequence. The cloning vector...
- Characterization of a chitinolytic enzyme from Serratia sp. KCK isolated from kimchi juice.
Hyun-Soo Kim; Timmis, Kenneth N.; Golyshin, Peter N. // Applied Microbiology & Biotechnology; Jul2007, Vol. 75 Issue 6, p1275
The novel chitinolytic bacterium Serratia sp. KCK, which was isolated from kimchi juice, produced chitinase A. The gene coding for the chitinolytic enzyme was cloned on the basis of sequencing of internal peptides, homology search, and design of degenerated primers. The cloned open reading frame...
- Distribution of Chitinases in the Entomopathogen Metarhizium anisopliae and Effect of N-Acetylglucosamine in Protein Secretion.
Barreto, Cristine Chaves; Staats, Charley Christian; Schrank, Augusto; Vainstein, Marilene Henning // Current Microbiology; Feb2004, Vol. 48 Issue 2, p102
For a long time, fungi have been characterized by their ability to secrete enzymes, mostly hydrolytic in function, and thus are defined as extracellular degraders. Chitin and chitinolytic enzymes are gaining importance for their biotechnological applications. Particularly, chitinases are used in...
- The functional effect of Gly209 and Ile213 substitutions on lysozyme activity of family 19 chitinase encoded by cyanophage Ma-LMM01.
Hosoda, Naohiko; Kurokawa, Yoichi; Sako, Yoshihiko; Nagasaki, Keizo; Yoshida, Takashi; Hiroishi, Shingo // Fisheries Science; Jul2011, Vol. 77 Issue 4, p665
ORF69 in the cyanophage infecting Microcystis aeruginosa, Ma-LMM01, shows homology to the family 19 chitinases where the catalytic domain has structural similarity to lysozyme. Chitinases hydrolyze chitin, a ï¿½-1, 4-linked monopolymer of N-acetylglucosamine (GlcNAc); whereas lysozymes...
- Evolution, Homology Conservation, and Identification of Unique Sequence Signatures in GH19 Family Chitinases.
Udaya Prakash, N. A.; Jayanthi, M.; Sabarinathan, R.; Kangueane, P.; Mathew, Lazar; Sekar, K. // Journal of Molecular Evolution; May2010, Vol. 70 Issue 5, p466
The discovery of GH (Glycoside Hydrolase) 19 chitinases in Streptomyces sp. raises the possibility of the presence of these proteins in other bacterial species, since they were initially thought to be confined to higher plants. The present study mainly concentrates on the phylogenetic...
- Insect chitinase and chitinase-like proteins.
Arakane, Yasuyuki; Muthukrishnan, Subbaratnam // Cellular & Molecular Life Sciences; Jan2010, Vol. 67 Issue 2, p201
Insect chitinases belong to family 18 glycosylhydrolases that hydrolyze chitin by an endo-type of cleavage while retaining the anomeric ï¿½-(1?4) configuration of products. There are multiple genes encoding chitinases and chitinase-like proteins in all insect species studied using...
- Control of Anthracnose by Chitosan through Stimulation of Defence-Related Enzymes in Eksotika II Papaya (Carica papaya L.) Fruit.
Ali, Asgar; Muda Mohamed, Mahmud Tengku; Siddiqui, Yasmeen // Journal of Biology & Life Science (JBLS); Aug2012, Vol. 3 Issue 1, p114
The ability of chitosan to induce disease resistance in harvested Eksotika II papaya fruit was investigated in relation to the activities of several defence-related enzymes. Harvested papaya fruit were dipped in various concentrations of chitosan solutions (0.5, 1.0, 1.5, 2.0%) along with an...
- Effect of Carbon Source on Production of Lytic Enzymes by Trichoderma Harzianum.
K���k, �igdem; Kivan�, Merih // Journal of Applied Biological Sciences; May2008, Vol. 2 Issue 2, p23
We investigated the effect of the different carbon sources and phytopathogenic fungal cell walls on production of enzymes by Turkish strain Trichoderma harzianum T15. Trichoderma harzianum T15 secretes ï¿½-1,3-glucanase and chitinase in the presence of different carbon sources. Maximal...
- Chitinase-Mediated Inhibitory Activity of Brassica Transgenic on Growth of Alternaria brassicae.
Mondal, Kalyan K.; Chatterjee, Subhas Chandra; Viswakarma, Navin; Bhattacharya, Ram Charan; Grover, Anita // Current Microbiology; Sep2003, Vol. 47 Issue 3, p0171
Chitinase, capable of degrading the cell walls of invading phytopathogenic fungi, plays an important role in plant defense response, particularly when this enzyme is overexpressed through genetic engineering. In the present study, Brassica plant (Brassica juncea L.) was transformed with...
- Transgenic alfalfa secretes a fungal endochitinase protein to the rhizosphere.
Tesfaye, Mesfin; Denton, Matthew D.; Samac, Deborah A.; Vance, Carroll P. // Plant & Soil; Feb2005, Vol. 269 Issue 1/2, p233
Transgenic plants containing a chimeric gene construct that facilitates the exudation of proteins from roots offer novel approaches for modification of the rhizosphere and production of relatively pure recombinant proteins. The aim of this study was to develop alfalfa (Medicago sativaL.) plants...
- Effects of dexamethasone or methotrexate on chitinolytic activity in nasal polyps.
Park, Seong Kook; Hur, Dae Young; Urm, Sang-Hwa // American Journal of Rhinology & Allergy; May/Jun2012, Vol. 26 Issue 3, p183
Background: Chitin is a recognition element for tissue infiltration by innate cells implicated in allergy and immunity. This process can be negatively regulated by vertebrate chitinases. Chitinolytic activity is significantly increased in nasal polyps (NPs) compared with that in normal turbinate...
- Production and partial characterization of chitinase from a halotolerant Planococcus rifitoensis strain M2-26.
Essghaier, Badiaa; Rouaissi, Mustapha; Boudabous, Abdellatif; Jijakli, Haissam; Sadfi-Zouaoui, Najla // World Journal of Microbiology & Biotechnology; Jun2010, Vol. 26 Issue 6, p977
This paper is the first to investigate the production and partial characterization of the chitinase enzyme from a moderately halophilic bacterium Planococcus rifitoensis strain M2-26, earlier isolated from a shallow salt lake in Tunisia. The impact of salt, salinity concentration, pH, carbon and...
- Plant Growth Promoting Characterization of Indigenous Azotobacteria Isolated from Soils in Iran.
Farajzadeh, Davoud; Yakhchali, Bagher; Aliasgharzad, Naser; Sokhandan-Bashir, Nemat; Farajzadeh, Malak // Current Microbiology; Apr2012, Vol. 64 Issue 4, p397
It has been well known that the bacteria of the genus Azotobacter, in addition to the beneficial N-fixing activity, are able to improve plant growth by a number of direct and indirect mechanisms. To identify this potential in indigenous azotobacteria, the efficiency of 17 isolates of Azotobacter...
- Evaluation of AMCase and CHIT-1 expression in monocyte macrophages lineage.
Rosa, Michelino; Gregorio, Corinne; Malaguarnera, Giulia; Tuttobene, Michele; Biazzo, Filomena; Malaguarnera, Lucia // Molecular & Cellular Biochemistry; Mar2013, Vol. 374 Issue 1/2, p73
Acidic mammalian chitinase (AMCase) and chitotriosidase (CHIT-1) are two active chitinases expressed in humans. The chitinase activity of AMCase was found to be causative in allergic inflammation and its expression was found to be induced by interleukin-13. CHIT1-1 is expressed by phagocytic...
- Sequence of the V. parahaemolyticus Gene for Cytoplasmic N,Nâ€²-Diacetylchitobiase and Homology with Related Enzymes1.
Wu, Michael H.; Laine, Roger A. // Journal of Biochemistry; 1999, Vol. 125 Issue 6, p1086
The nucleotide sequence of the gene encoding the cytoplasmic N,Nâ€²-diacetylchitobiase [EC 188.8.131.52] from Vibrio parahaemolyticus (ATCC #27969) has been determined. The deduced peptide sequence of this unusual Î²-hexosaminidase surprisingly shows minimum evolutionary relationship to two...
- Simplified Method of Preparing Colloidal Chitin Used For Screening of Chitinase-Producing Microorganisms.
Murthy, N. K. S.; Bleakley, B. H. // Internet Journal of Microbiology; 2012, Vol. 10 Issue 2, p7
A simplified and efficient method of preparing colloidal chitin from inexpensive crab shell flakes was developed. It modifies some steps of existing techniques to provide significant saving in effort and materials for colloidal chitin preparation. In colloidal chitin preparation, unless the...
- DNA variation in the basic chitinase locus (ChiB) region of the wild plant Arabidopsis thaliana.
Kawabe, Akira; Miyashita, Naohiko T. // Genetics; Nov99, Vol. 153 Issue 3, p1445
Discusses intraspecific nucleotide variation in a 2.2-kbp region of basic chitinase (ChiB) region in Arabidopsis thaliana. Study of genetic mechanisms involved in DNA polymorphism in A. thaliana; Characteristics of the chitinase system as having multiple genes of similar function but distinct...
- ENZYMATIC PROCESSING OF CHITINACEOUS WASTES FOR N-ACETYL-D-GLUCOSAMINE PRODUCTION: AN EXAMPLE OF GREEN AND EFFICIENT ENVIRONMENTAL MANAGEMENT.
Das, Sancharini; Sen, Ramkrishna; Roy, Debasis // Environmental Engineering & Management Journal (EEMJ); Oct2012, Vol. 11 Issue 10, p1849
Chitin from chitinaceous prawn shell waste, fungal biomass of Aspergillus fumigatus and Penicillium waksmanii was used as natural substrate for partially purified chitinase of Trichoderma harzianum to produce N-acetyl-D-glucosamine (GlcNAc), a medically important amino sugar. The isolated...
- Novel Biological Activities of Allosamidins.
Shohei Sakuda; Hiromasa Inoue; Hiromichi Nagasawa // Molecules; Jun2013, Vol. 18 Issue 6, p6952
Allosamidins, which are secondary metabolites of the Streptomyces species, have chitin-mimic pseudotrisaccharide structures. They bind to catalytic centers of all family 18 chitinases and inhibit their enzymatic activity. Allosamidins have been used as chitinase inhibitors to investigate the...
- Purification and Characterization of an Antifungal Chitinase in Jelly Fig (Ficus awkeotsang) Achenes.
Yu-Ching Li; Chen-Tien Chang; Eric S. L. Hsiao; Joyce S. F. Hsu; Jenn-Wen Huang; Jason T. C. Tzen // Plant & Cell Physiology; Nov2003, Vol. 44 Issue 11, p1162
A method was developed to purify a 30-kDa protein from jelly fig (Ficus awkeotsang) pericarp, including preparation of jelly curd from achenes, extraction of proteins from the curd, and isolation of the 30-kDa protein by anion-exchanger and gel filtration. Chitinase activity was detected in the...
- EXTRACTION AND PARTIAL PURIFICATION OF ISOFORMS OF CHITINASE FROM LEAVES OF BETA-VULGARIS.
GAIKWAD, U. A.; SHEMBEKAR, V. S. // Indian Streams Research Journal; Jan2013, Vol. 2 Issue 12, Special section p1
The enzyme was extracted from leaves of beet root and obtained the best Chitinase at optimum extracting condition with water at 20ocfor 90 min. Chitinase from leaves of sugar beet was purified by( NH4)2SO4 precipitation 20 to 60 %. Further purification was carried by dialysis to minimize protein...
- Effect of Agitation and Aeration Rates on Chitinase Production Using Trichoderma virens UKM1 in 2-l Stirred Tank Reactor.
Christine Fernandez; Madihah Salleh; Rosli Illias; Mohd. Hassan // Applied Biochemistry & Biotechnology; Aug2008, Vol. 150 Issue 2, p193
AbstractÂ Â Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-d-glucosamine (GluNac), a reducing sugar....