TITLE

Establishment of an agroinoculation system for broad bean wilt virus 2

AUTHOR(S)
Atsumi, Go; Tomita, Reiko; Kobayashi, Kappei; Sekine, Ken-Taro
PUB. DATE
July 2013
SOURCE
Archives of Virology;Jul2013, Vol. 158 Issue 7, p1549
SOURCE TYPE
Academic Journal
DOC. TYPE
Report
ABSTRACT
We determined the complete nucleotide sequence of a broad bean wilt virus 2 (BBWV-2) isolate from gentian in Japan. The full-length RNA1 and RNA2 sequences, excluding poly(A) tails, were 5955 and 3600 nucleotides long, respectively. Analysis indicated that, in contrast to other BBWV-2 isolates, the 5' end of both RNA1 and RNA2 starts with a GUU sequence. We successfully inoculated Nicotiana benthamiana with BBWV-2 by infiltrating a mixed suspension of two Agrobacterium tumefaciens clones carrying binary vectors with the full-length RNA1 and RNA2 sequences. This is the first report on the efficient, easy and high-throughput use of agroinoculation for generating BBWV-2 infections.
ACCESSION #
88428261

 

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