TITLE

Real-Time Polymerase Chain Reaction

AUTHOR(S)
Vohr, Hans-Werner
PUB. DATE
January 2005
SOURCE
Encyclopedic Reference of Immunotoxicology;2005, p549
SOURCE TYPE
Book
DOC. TYPE
Reference Entry
ABSTRACT
A definition of the term real-time polymerase chain reaction is presented. It refers to a system that is capable of detecting and quantifying gene expression or concentration of a pathogen. Thermal cycling, fluorescence detection and application-specific software are important to monitor the products developed form polymerase chain reaction.
ACCESSION #
21874708

 

Related Articles

  • A simple device using magnetic transportation for droplet-based PCR. Tetsuo Ohashi; Hiroki Kuyama; Nobuhiro Hanafusa; Yoshiyuki Togawa // Biomedical Microdevices;Oct2007, Vol. 9 Issue 5, p695 

    Abstract  The Polymerase chain reaction (PCR) was successfully and rapidly performed in a simple reaction device devoid of channels, pumps, valves, or other control elements used in conventional lab-on-a-chip technology. The basic concept of this device is the transportation of...

  • Chapter 7: Nucleic Acid Amplification. Buckingham, Lela; Flaws, Maribeth L. // Molecular Diagnostics: Fundamentals, Methods & Clinical Applicat;2007, p121 

    The article discusses the assays for amplifying nucleic acids which includes polymerase chain reaction, branched DNA amplification, ligase chain reaction. It also describes the detection of amplicons for the amplification methods. Comparison between target amplification and signal amplification...

  • PCR: The Amplifier Heard 'Round The World. Dove, Alan // Bioscience Technology;Jul2007, Vol. 32 Issue 7, p1 

    The article offers facts about polymerase chain reaction (PCR). The quantitative real-time PCR is fast becoming the standard DNA amplification method in many laboratories. A typical real-time PCR system includes a standard PCR thermal cycler, plus a fluorescence reader and associated hardware...

  • Detection of Recombinant Products during PCR Amplification of DNA Containing Direct Alu Repeats. Shibalev, D. V.; Voronov, A. S.; Bashkirov, V. N.; Kupriyanova, N. S.; Ryskov, A. P. // Doklady Biochemistry & Biophysics;Jan/Feb2003, Vol. 388 Issue 1-6, p55 

    Presents information on a study which examined the detection of recombinant products during polymerase chain reaction (PCR) amplification of DNA containing direct alu repeats. Recombinant products formed during PCR amplification; Study methods; Results.

  • Use of degenerate primers and heat-soaked polymerase chain reaction (PCR) to clone a serine protease antigen from Dermatophilus congolensis. Mine, Ontiretse M.; Carnegie, Patrick R. // Immunology & Cell Biology;Oct1997, Vol. 75 Issue 5, p484 

    Serine proteases arc thought to be involved in the initial attack on sheep skin by Dermatophilus congolensis and are obvious antigens for inclusion in a vaccine to prevent lumpy wool disease (dermatophilosis). Degenerate primers were designed after alignment of seven bacterial serine proteases....

  • HER2 Gene Amplification and Chromosome 17 Copy Number Do Not Predict Survival of Patients with Resected Pancreatic Adenocarcinoma. Ramesh Ramanathan; Kathleen Cieply; Alyssa Krasinskas // Digestive Diseases & Sciences;Nov2008, Vol. 53 Issue 11, p3026 

    Abstract  HER2 gene amplification is an established predictive and prognostic marker in breast cancer. Since there are conflicting reports as to the significance of HER2 gene amplification in pancreatic cancer, we undertook this study. We studied HER2 gene amplification, HER2...

  • Three endochitinase-encoding genes identified in the biocontrol fungus Clonostachys rosea are differentially expressed. Mamarabadi, Mojtaba; Jensen, Birgit; Lübeck, Mette // Current Genetics;Aug2008, Vol. 54 Issue 2, p57 

    Three endochitinase-encoding genes, cr-ech58, cr-ech42 and cr-ech37 were identified and characterised from the mycoparasitic C. rosea strain IK726. The endochitinase activity was specifically induced in media containing chitin or Fusarium culmorum cell walls as sole carbon sources. RT-PCR...

  • Potential for Predicting Toxicity and Response of Fluoropyrimidines in Patients. Eliason, James F.; Megyeri, Attila // Current Drug Targets;May2004, Vol. 5 Issue 4, p383 

    The efficacy of cancer therapy is compromised by the fact that there are currently no good ways to predict which patients will benefit from treatment. This long standing goal is closer to becoming a reality as more is learned about the molecules that affect the activities of various therapeutic...

  • Humans learn to copy DNA.  // History of Science & Technology;2004, p662 

    The article presents information on the polymerase chain reaction (PCR). Developed by Kary Mullis, PCR or gene amplification is used in making multiple copies of DNA molecule. It made use of the enzyme DNA polymerase to copy line of code. The whole process requires warm temperatures to separate...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics