Southern Blot test

January 2005
Taber's Cyclopedic Medical Dictionary;2005, p2029
Reference Entry
A definition of the term "Southern Blot test" is presented. It refers to the analytical method, which is traditionally used in DNA analysis. The test is applied after a sample of DNA fragments is separated by agarose gel electrophoresis. Then, the fragments are transferred to a solid cellulose support by blotting. Capillary action draws the fragments onto the solid support. The support is then treated with radiolabeled DNA probes.


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