Pitfalls encountered while investigating genetic elements by PCR

Palmieri, Claudio; Brenciani, Andrea; Mingoia, Marina; Giovanetti, Eleonora; Facinelli, Bruna; Varaldo, Pietro E.
May 2013
Mobile Genetic Elements (2159-2543);May/Jun2013, Vol. 3 Issue 3, p1
Academic Journal
The authors reflect on the difficulties that are encountered while investigating genetic elements by polymerase chain reaction (PCR). According to them, the use of PCR-based techniques to study new genetic elements (GEs) requires careful consideration of the possible different PCR targets associated with different subpopulations in order to avoid incorrect or incomplete interpretations.


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