Abnormal development of the enteric nervous system in rat embryos and fetuses with congenital diaphragmatic hernia

Martínez, Leopoldo; Aras-López, Rosa; Lancha, Sara; Vallejo-Cremades, María; Pederiva, Federica; XiaoMei, Liu; Tovar, Juan; Martínez, Leopoldo; Aras-López, Rosa; Vallejo-Cremades, María Teresa; Tovar, Juan Antonio
February 2011
Pediatric Surgery International;Feb2011, Vol. 27 Issue 2, p165
Academic Journal
journal article
Background/aim: Esophageal dilatation, gastroesophageal reflux, and intestinal obstruction have been demonstrated in CDH survivors. Abnormal esophageal and intestinal innervations were recently found in rats and babies with this disease. Our aim was to further characterize these malformations in embryos and fetal rats exposed to nitrofen.Methods: Pregnant rats received either 100 mg nitrofen or vehicle on E9.5. Fetuses were recovered at E15, E18, and E21. Sections of esophagus and small bowel were histochemically stained with acetylcholinesterase (AChE) and immunostained for PGP9.5. PGP9.5 gen protein were measured on E21 and PGP9.5 mRNA on E15, E18 and E21. Comparisons between groups were made with non-parametrics tests.Results: Histochemistry and immunohistochemistry showed deficient innervation in all anatomical areas studied at E15, E18, and E21, and WB confirmed this decrease in E21 fetuses. PGP9.5 messenger was decreased in nitrofen-exposed animals on E18 (esophagus) or E15 (small bowel), and increased on E21 in the esophagus and E18 in small bowel.Conclusions: Development of the enteric nervous system of the esophagus, stomach, and small bowel is deficient in rat embryos and fetuses exposed to nitrofen. These anomalies could account in part for the long-term gastrointestinal morbidity observed in CDH survivors.


Related Articles

  • Pneumococcus activation of the 5-lipoxygenase pathway and production of glycoproteins in the middle ear of rats. Lin, Jizhen; Vambutas, Andrea; Haruta, Atsushi; Paparella, Michael M.; Giebink, G. Scott; Youngki Kim; Lin, J; Vambutas, A; Haruta, A; Paparella, M M; Giebink, G S; Kim, Y // Journal of Infectious Diseases;5/1/99, Vol. 179 Issue 5, p1145 

    Pneumococcal otitis media is associated with the production of potent inflammatory mediators (leukotrienes), but the mechanism by which pneumococcus induces production of leukotrienes in the middle ear is poorly understood. In this study, up-regulation of 2 genes that govern the lipoxygenase...

  • Pre-treatment with N-acetylcysteine upregulates superoxide dismutase 2 and catalase genes in cadmium-induced oxidative stress in the chick omphalocele model. Doi, Takashi; Puri, Prem; Bannigan, John; Thompson, Jennifer // Pediatric Surgery International;Feb2011, Vol. 27 Issue 2, p131 

    Purpose: In the chick embryo, administration of the heavy metal Cadmium (Cd) induces omphalocele phenotype. Cd is a potent inhibitor of antioxidant enzymes and causes accumulation of reactive oxygen species (ROSs) such as hydrogen peroxide. Previous work with the Cd chick model has...

  • Changes of renal AT1/AT2 receptors and structures in ovine fetuses following exposure to long-term hypoxia. Mao, Caiping; Hou, Jianquan; Ge, Jianyi; Hu, Yali; Ding, Yang; Zhou, Yun; Zhang, Huiying; Xu, Zhice; Zhang, Lubo // American Journal of Nephrology;2010, Vol. 31 Issue 2, p141 

    Background/aims: The present study tested the hypothesis that chronic hypoxia adversely affects renal development in the ovine fetus.Methods: Kidneys were collected from near-term fetuses of pregnant ewes maintained at sea level or high altitude (3,801 m, PaO(2):...

  • Age-Associated Changes in Gene Expression Patterns in the Liver Thomas, Robert P.; Guigneaux, Michelle; Wood, Thomas; Evers, B. Mark // Journal of Gastrointestinal Surgery;May2002, Vol. 6 Issue 3, p445 

    Aging is one of the least clearly understood biological processes. Alteration of oxidation/reduction (redox) enzymes has been demonstrated with aging; however, a systematic analysis of expression patterns has not been performed. The liver plays a key role in homeostasis and detoxification;...

  • The Expression Analysis of Nanog in the Developing Rat Myocardial Tissues. Guo, Zhi-kun; Guo, Kang; Luo, Huanhuan; Mu, Ling-min; Li, Qiong; Chang, Yu-qiao // Cellular Physiology & Biochemistry (Karger AG);Feb2015, Vol. 35 Issue 3, p866 

    Objectives: To investigate the expression dynamic of nanog gene in the development of rat myocardial tissues. Methods: SD rats were studied at 5 time points before and after birth. The techniques of immunohistochemistry, immunofluorescence, western blotting and RT-PCR were used to investigate...

  • Expression of the Wilm's tumor gene WT1 during diaphragmatic development in the nitrofen model for congenital diaphragmatic hernia. Dingemann, Jens; Doi, Takashi; Ruttenstock, Elke; Puri, Prem // Pediatric Surgery International;Feb2011, Vol. 27 Issue 2, p159 

    Purpose: The nitrofen model of congenital diaphragmatic hernia (CDH) reproduces a typical diaphragmatic defect. However, the exact pathomechanism of CDH is still unknown. The Wilm's tumor 1 gene (WT1) is crucial for diaphragmatic development. Mutations in WT1 associated with CDH...

  • Gene-Selective Histone H3 Acetylation in the Absence of Increase in Global Histone Acetylation in Liver of Rats Chronically Fed Alcohol. Park, Pil-Hoon; Lim, Robert W.; Shukla, Shivendra D. // Alcohol & Alcoholism;May/Jun2012, Vol. 47 Issue 3, p233 

    Aims: The aim of this study was to determine the effect of chronic ethanol feeding on acetylation of histone H3 at lysine 9 (H3-Lys9) at promoter and coding regions of genes for class I alcohol dehydrogenase (ADH I), inducible nitric oxide synthase (iNOS), Bax, p21, c-met and hepatocyte growth...

  • Nitric oxide synthase inhibition delays lowfrequency stimulation-induced satellite cell activation in rat fast-twitch muscle. Martins, Karen J. B.; MacLean, Ian; Murdoch, Gordon K.; Dixon, Walter T.; Putman, Charles T. // Applied Physiology, Nutrition & Metabolism;Dec2011, Vol. 36 Issue 6, p996 

    This study examined the effect of nitric oxide synthase (NOS) inhibition via Nu-nitro-L-arginine methyl ester (L-NAME) administration on low-frequency stimulation-induced satellite cell (SC) activation in rat skeletal muscle. L-NAME only delayed stimulation-induced increases in SC activity....

  • Contractile activity-induced gene expression in fast- and slow-twitch muscle. Nguyen, Linda M.-D. // Applied Physiology, Nutrition & Metabolism;Apr2011, Vol. 36 Issue 2, p233 

    Many proteins that function as transcription factors regulate the transcriptional activity of nuclear genes encoding mitochondrial proteins. Several of these are rapidly inducible with contractile activity, followed by a recovery phase. The aim of the present study was to evaluate the expression...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics