TITLE

Identification of HLA-DR-bound peptides presented by human bronchoalveolar lavage cells in sarcoidosis

AUTHOR(S)
Wahlström, Jan; Dengjel, Jörn; Persson, Bengt; Duyar, Hüseyin; Rammensee, Hans-Georg; Stevanović, Stefan; Eklund, Anders; Weissert, Robert; Grunewald, Johan; Wahlström, Jan; Dengjel, Jörn; Duyar, Hüseyin; Stevanović, Stefan
PUB. DATE
November 2007
SOURCE
Journal of Clinical Investigation;Nov2007, Vol. 117 Issue 11, p3576
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
Sarcoidosis is an inflammatory disease of unknown etiology, most commonly affecting the lungs. Activated CD4+ T cells accumulate in the lungs of individuals with sarcoidosis and are considered to be of central importance for inflammation. We have previously shown that Scandinavian sarcoidosis patients expressing the HLA-DR allele DRB1*0301 are characterized by large accumulations in the lungs of CD4+ T cells expressing the TCR AV2S3 gene segment. This association afforded us a unique opportunity to identify a sarcoidosis-specific antigen recognized by AV2S3+ T cells. To identify candidates for the postulated sarcoidosis-specific antigen, lung cells from 16 HLA-DRB1*0301pos patients were obtained by bronchoalveolar lavage. HLA-DR molecules were affinity purified and bound peptides acid eluted. Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. We identified 78 amino acid sequences from self proteins presented in the lungs of sarcoidosis patients, some of which were well-known autoantigens such as vimentin and ATP synthase. For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. This approach can be extended to characterize HLA-bound peptides in various autoimmune settings.
ACCESSION #
27477544

 

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