The effects of biocides (antiseptics and disinfectants) on the endospores of Rhinosporidium seeberi

Arseculeratne, S. N.; Atapattu, D. N.; Balasooriya, P.; Fernando, R.
April 2006
Indian Journal of Medical Microbiology;Apr2006, Vol. 24 Issue 2, p85
Academic Journal
journal article
No data exists on the activity of biocides (antiseptics and disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis in humans and animals. On account of the inability to culture R. seeberi, in vitro, dyes were used to assess the morphological integrity and viability of biocide-treated endospores that are considered to be the infective stage of this pathogen. Evan's Blue (EvB) identifies the morphological integrity of the endospores while MTT (3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide) identifies metabolic activity through its reduction by cellular dehydrogenases to microscopically visible deposits of insoluble formazan. MTT-negativity has earlier been shown to correlate with absence of growth of yeast and mycelial fungi in culture and could thus indicate the loss of viability of MTT-negative rhinosporidial endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol, chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70% ethanol, 10% formalin, povidone-iodine, sodium azide and silver nitrate were tested on freshly-harvested endospores and all biocides caused metabolic inactivation with or without altered structural integrity as shown by absence of MTT-staining after 3, 24 or 36 hour after exposure, while EvB stained only the endospores treated with sodium azide, ethanol, thimerosal, chloroxylenol, glutaraldehyde and hydrogen peroxide. With clinically useful biocides - chlorhexidine, cetrimide-chlorhexidine, 70% ethanol, povidone-iodine and silver nitrate, a total period of exposure of endospores to the biocide, for seven minutes, produced metabolic inactivation of the endospores. Anti-rhinosporidial antiseptics that could be used in surgery on rhinosporidial patients include povidone-iodine in nasal packs for nasal and naso-pharyngeal surgery, chlorhexidine and cetrimide-chlorhexidine on the skin, while povidone-iodine and silver nitrate could have application in ocular rhinosporidiosis.


Related Articles

  • Antiseptic.  // A-Z of Food Safety;2007, p14 

    A definition of the term "antiseptic" is presented. Generally, it refers to a chemical substance which prevents the growth of bacteria and mould particularly in or on the human body.

  • nitromersol.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p1589 

    A definition of the medical term "nitromersol," which refers to an organic mercurial antiseptic, is presented.

  • aseptic-antiseptic.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p196 

    A definition of the term "aseptic-antiseptic" is presented.

  • Destruction of Alicyclobacillus acidoterrestris Spores in Apple Juice on Stainless Steel Surfaces by Chemical Disinfectants. PODOLAK, RICHARD; ELLIOTT, PHILIP H.; TAYLOR, BRADLEY J.; KHURANA, AAKASH; BLACK, DARRYL G. // Journal of Food Protection;Mar2009, Vol. 72 Issue 3, p510 

    A study was conducted to determine the effects of three commercially available disinfectants on the reduction of Alicyclobacillus acidoterrestris spores in single-strength apple juice applied to stainless steel surfaces. Apple juice was inoculated with A. acidoterrestris spores, spread onto the...

  • Application of Gaseous Ozone for Inactivation of Bacillus subtilis Spores. Aydogan, Ahmet; Gurol, Mirat D. // Journal of the Air & Waste Management Association (Air & Waste M;Feb2006, Vol. 56 Issue 2, p179 

    The effectiveness of gaseous ozone (O3) as a disinfectant was tested on Bacillus subtilis spores, which share the same physiological characteristics as Bacillus anthracis spores that cause the anthrax disease. Spores dried on surfaces of different carrier material were exposed to O3 gas in the...

  • Mushrooms. Churchman, Deborah // Ranger Rick;Sep2002, Vol. 36 Issue 9, p21 

    Features several kinds of fungi. Characteristics of mushrooms; Role of spores in the reproduction of another specie of fungi; Habitat of fungi; Number of recorded species of mushrooms.

  • disinfection, high-level.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p665 

    A definition of the term "high-level disinfection" which refers to the inactivation of all bacteria, fungi, mycobacteria, and viruses on an instrument or surface used in patient care is presented.

  • Longitudinal analysis of chlorhexidine susceptibilities of nosocomial methicillin-resistant Staphylococcus aureus isolates at a teaching hospital in Taiwan. Jann-Tay Wang; Wang-Huei Sheng; Jiun-Ling Wang; Duckling Chen; Mei-Ling Chen; Yee-Chun Chen; Shan-Chwen Chang // Journal of Antimicrobial Chemotherapy (JAC);Sep2008, Vol. 62 Issue 3, p514 

    : Background Chlorhexidine has been widely used for hand hygiene to prevent transmission of nosocomial pathogens, including methicillin-resistant Staphylococcus aureus (MRSA). However, data on longitudinal surveillance of the susceptibility of MRSA isolates to chlorhexidine are limited. :...

  • Antifungal agents: susceptibility of different fungal strains to various disinfecting agents. Staniszewska, Monika; Jakimiak, Bożenna; Röhm-Rodowald, Ewa; Bocian, Ewa; Kurzątkowski, Wieslaw; Tyski, Stefan // Medical Mycology / Mikologia;2009, Vol. 16 Issue 4, p210 

    The aim of the study was to evaluate the activity of biocides against wide spectrum of clinical fungal strains and to examine whether disinfectants applied in hospital areas are active against the selected most resistant strains. Material and methods: The activity of the 4 disinfectants and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics