TITLE

Biochemical, Histopathological and Immunohistochemical Evaluation of the Protective and Therapeutic Effects of Thymoquinone against Ischemia and Ischemia/Reperfusion Injury in the Rat Ovary

AUTHOR(S)
Mete Ural, Ülkü; Bayoğlu Tekin, Yeşim; Şehitoğlu, İbrahim; Kalkan, Yıldıray; Cumhur Cüre, Medine
PUB. DATE
January 2016
SOURCE
Gynecologic & Obstetric Investigation;Jan2016, Vol. 81 Issue 1, p47
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
Aim: To evaluate the antioxidant effects of thymoquinone (TQ) and to investigate the biochemical, histopathological and immunohistochemical changes in experimental rat ovarian torsion.Methods: A total of 48 female adult rats were used in this study and randomly divided into 7 groups: (1) sham operation; (2) bilateral 3-hour ovarian ischemia; (3) 3-hour ischemia and 3-hour reperfusion; (4) and (5) rats were administered 20 and 40 mg/kg of TQ, respectively, before 0.5 h of ischemia, and then 3 h of ovarian ischemia was applied; (6) and (7) 3-hour ovarian ischemia was applied; 2.5 h after the induction of ischemia, rats were administered the same doses of TQ; at the end of 3 h of ischemia, a 3-hour reperfusion was applied. Histologic changes under light microscopy, immunoreactivity for anticaspase-3 and serum levels of malondialdehyde, interleukin-6, catalase and glutathione peroxidase were noted and compared between the 7 groups.Results: Ischemia and ischemia/reperfusion cause a deterioration of biochemical and histopathological parameters. Administration of TQ seems to reverse these alterations and alleviate the injury. Antioxidant defense mechanisms appear to be enhanced by the administration of TQ.Conclusion: TQ at different doses attenuates ovarian ischemia and ischemia/reperfusion injury in rats.
ACCESSION #
112462092

 

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