The effects of varying oxygen conditions and immunoglobulin A on barrier defense to bacterial invasion

Baylor III, Alfred E.; Diebel, Lawrence N.; Liberati, David M.; Dulchavsky, Scott A.; Diglio, Clement A.; Brown, William J.; Baylor, Alfred E
March 2003
American Surgeon;Mar2003, Vol. 69 Issue 3, p231
Academic Journal
journal article
Tissue oxygenation is a critical factor in host defense against bacteria. Gut mucosal tissue oxygenation (partial pressure of O2) is normally low putting the gut at risk of invasion by luminal microbes. Secretory immunoglobulin (Ig) A (sIgA) is the principal immune defense at mucosal surfaces. The protective effect of IgA under low oxygen conditions is unknown. We studied the interaction of varying O2 environments and sIgA on protection against bacterial invasion in our in vitro model. Cell monolayers of Madin-Darby canine kidney (MDCK) cells transfected with the cDNA for polymeric immunoglobulin receptor were established in a two-chamber cell culture system. A commensal strain of Escherichia coli (10(8) colony-forming units) was added to the apical medium and cell cultures were placed in either a 5, 21, or 95 per cent O2 environment at 37 degrees C. Polyclonal sIgA (100 microg/mL) was added to the apical chamber in subsets. Basal medium was sampled at intervals and bacterial translocation quantitated. The cell monolayers of MDCK transfected cells then had 100 microg/mL IgA added to the basal compartment at 4 degrees C for 2 hours followed by various oxygen environments for 90 minutes. Afterwards apical medium was removed at one, 3, and 12 (overnight) hours. The bacterial translocation data showed a significance increase in translocation with hypoxia. Both increased oxygen and IgA abrogated these effects significantly. The transcytosis of IgA was increased during hypoxic conditions. Normal and hyperoxic conditions did not produce any significant difference in IgA transcytosis. We conclude that O2 and sIgA are protective against bacterial invasion at epithelial surfaces. Effects to either boost O2 delivery to the gut or enhance mucosal IgA production and delivery may be protective in the critically ill surgical patient.


Related Articles

  • Bacterial migration through punctured surgical gloves under real surgical conditions. Hübner, Nils-Olaf; Goerdt, Anna-Maria; Stanislawski, Natalie; Assadian, Ojan; Heidecke, Claus-Dieter; Kramer, Axel; Partecke, Lars Ivo // BMC Infectious Diseases;2010, Vol. 10 Issue 1, p192 

    Background: The aim of this study was to confirm recent results from a previous study focussing on the development of a method to measure the bacterial translocation through puncture holes in surgical gloves under real surgical conditions. Methods: An established method was applied to detect...

  • Effect of Fluconazole on Phagocytic Response of Polymorphonuclear Leukocytes in a Rat Model of Acute Sepsis. Khan, Haseeb Ahmad // Mediators of Inflammation;2/24/2005, Vol. 2005 Issue 1, p9 

    The article examines the effect of Fluconazole on phagocytic response of polymorphonuclear leukocytes (PMNs) in a rat model of acute sepsis. Sepsis and its sequelae are often lethal and considered to be the leading causes of mortality in intensive care units. The cascade of events initiating...

  • Plant immunity: Helping plants to fight back. Molloy, Sheilagh // Nature Reviews Microbiology;May2010, Vol. 8 Issue 5, p312 

    The article highlights a study which investigates whether the elongation factor-Tu (EF-Tu) receptor (EFR) could be used to increase the resistance of a plant to bacterial infection. Researchers found that EFR has recognized an 18-residue peptide in EF-Tu that known as elf18. They examined that...

  • Experimental Infections with Mycoplasma agalactiae Identify Key Factors Involved in Host-Colonization. Baranowski, Eric; Bergonier, Dominique; Sagné, Eveline; Hygonenq, Marie-Claude; Ronsin, Patricia; Berthelot, Xavier; Citti, Christine // PLoS ONE;Apr2014, Vol. 9 Issue 4, p1 

    Mechanisms underlying pathogenic processes in mycoplasma infections are poorly understood, mainly because of limited sequence similarities with classical, bacterial virulence factors. Recently, large-scale transposon mutagenesis in the ruminant pathogen Mycoplasma agalactiae identified the NIF...

  • Indications for the Immunological Evaluation of Patients with Meningitis. Overturf, Gary D. // Clinical Infectious Diseases;1/15/2003, Vol. 36 Issue 2, p189 

    Although people with bacterial meningitis lack adequate protective antibody against the invading pathogen, most do not have an underlying immunodeficiency. Certain comorbid conditions increase the risk for development of bacterial sepsis and meningitis. In addition, certain congenital complement...

  • Disseminated mycobacteriosis caused by Battey type mycobacteria. Koenig, M. Glenn; Collins, Robert; Heyssel, Robert; Koenig, M G; Collins, R D; Heyssel, R M // Annals of Internal Medicine;Jan66, Vol. 64 Issue 1, p145 

    Describes a case of disseminated Battery bacillus infection occurring in a 62-year-old woman. Medical history of the patient; Result of the patient's physical examination; Autopsy findings.

  • bacteriogenic.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p230 

    A definition of the term "bacteriogenic," which refers to either a condition caused by bacteria or producing bacteria, is presented.

  • bacteriosis.  // Taber's Cyclopedic Medical Dictionary (2009);2009, Issue 21, p230 

    A definition of the term "bacteriosis," which refers to any disease caused by bacteria, is presented.

  • The Enterococcus: 'Putting the Bug in Our Ears'. Hoffman, Stephen A.; Mollering Jr., Robert C. // Annals of Internal Medicine;May87, Vol. 106 Issue 5, p757 

    Focuses on a study by M.J. Zervos and colleagues regarding the frequency of colonization and infection of the organism enterococci demonstrating probable person-to-person spread. Virulence and incidence of infection; Antimicrobial susceptibility; Epidemiology of gentamicin-resistant...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics