Experimental design for optimization of microwave-assisted extraction of benzodiazepines in human plasma

Fern�ndez, P.; V�zquez, C.; Lorenzo, R.; Carro, A.; �lvarez, I.; Cabarcos, P.
May 2010
Analytical & Bioanalytical Chemistry;May2010, Vol. 397 Issue 2, p677
Academic Journal
A simple and fast microwave-assisted-extraction (MAE) method has been evaluated as an alternative to solid-phase extraction (SPE) for the determination of six benzodiazepines widely prescribed in European countries (alprazolam, bromazepam, diazepam, lorazepam, lormetazepam and tetrazepam) in human plasma. For MAE optimization a Doehlert experimental design was used with extraction time, temperature and solvent volume as influential parameters. A desirability function was employed in addition to the simultaneous optimization of the MAE conditions. The analysis of variance showed that the solvent volume had a positive influence on the extraction of all the analytes tested, achieving a statistically significant effect. Also, the extraction time had a statistically significant effect on the extraction of four benzodiazepines. The selected MAE conditions�89 �C, 13 min and 8 mL of chloroform/2-propanol (4:1, v/v)�led to recoveries between 89.8 � 0.3 and 102.1 � 5.2% for benzodiazepines using a high performance liquid chromatography method coupled with diode-array detection. The comparison of MAE and SPE shows better results for MAE, with a lower number of steps in handling the sample and greater efficiency. The applicability of MAE was successfully tested in 27 plasma samples from benzodiazepine users.


Related Articles

  • Response surface methodology for the microwave-assisted extraction of insecticides from soil samples. Hernández-Soriano, M. Carmen; Peña, Araánzazu; Mingorance, M. Dolores // Analytical & Bioanalytical Chemistry;Sep2007, Vol. 389 Issue 2, p619 

    The extraction of two pyrethroid insecticides (deltamethrin and α-cypermethrin) together with three organophosphorus insecticides (dimethoate, diazinon and malathion) from soil samples was carried out with microwave-assisted technology. Experimental designs showed that extraction temperature,...

  • Study on microwaved-assisted extraction of auricularia auricular polysaccharide. Wang Xiao-jun; Li Ying // Basic Sciences Journal of Textile Universities / Fangzhi Gaoxia;Mar2010, Vol. 23 Issue 1, p95 

    A microwave-assisted extraction (MAE) method was studied for the extraction of polysaccharides. Auricular polysaccharides was extracted by microwave-assisted by water as the solvent. The experimental factors, such as microwave power, irradiation time, ration of solid to solvent and extraction...

  • Box-Behnken Design for Investigation of Microwave-Assisted Extraction of Patchouli Oil. Kusuma, Heri Septya; Mahfud, Mahfud // AIP Conference Proceedings;2015, Vol. 1699 Issue 1, p1 

    Microwave-assisted extraction (MAE) technique was employed to extract the essential oil from patchouli (Pogostemon cablin). The optimal conditions for microwave-assisted extraction of patchouli oil were determined by response surface methodology. A Box-Behnken design (BBD) was applied to...

  • Mikrodalga Destekli Soxhlet Cihazı ile Fındık Yağı Ekstraksiyonunun Yanıt Yüzey Yöntemi ile Optimizasyonu. Tunç, İlknur; Çalışkan, Firdevs; Özkan, Gülcan; Karacabey, Erkan // Academic Food Journal / Akademik GIDA;oca-mar2014, Vol. 12 Issue 1, p20 

    Hazelnut is an important food in terms of the high proportion of oil and oil bioactive components in the composition of oil. In this study, the potential use of the microwave-assisted Soxhiet system (green extraction technique) for the production of maximum oil yield from hazelnut and oil with...

  • Microwave-assisted extraction and large-volume injection gas chromatography tandem mass spectrometry determination of multiresidue pesticides in edible seaweed. García-Rodríguez, D.; Carro, A. M.; Cela, R.; Lorenzo, R. A. // Analytical & Bioanalytical Chemistry;Sep2010, Vol. 398 Issue 2, p1005 

    microwave-assisted extraction method followed by clean-up with solid-phase extraction (SPE) combined with large-volume injection gas chromatography–tandem mass spectrometry (LVI-GC-MS/MS) for the analysis of 17 pesticides in wild and aquaculture edible seaweeds has been developed. An...

  • Mercury speciation analysis in human hair by species-specific isotope-dilution using GC-ICP-MS. Laffont, Laure; Maurice, Laurence; Amouroux, David; Navarro, Patricia; Monperrus, Mathilde; Sonke, Jeroen; Behra, Philippe // Analytical & Bioanalytical Chemistry;Mar2013, Vol. 405 Issue 9, p3001 

    We optimized a mercury (Hg) speciation extraction method for human hair in combination with species-specific isotope-dilution analysis by gas chromatography-inductively coupled plasma-mass spectrometry (GC-ICP-MS). The method was validated on human hair reference material RM (IAEA-086), which is...

  • Microwave-Assisted Extraction of Polysaccharide from Sargassum thunbergii. Li Qing-tao; Pan Lu-lu; Huang Kang-ning; Du Yu-lan; Li Zhen-yu // Natural Product Research & Development;Dec2011, Vol. 23 Issue 6, p1160 

    In this study, the microwave-assisted method was used to extract polysaccharides from Sargassum thunbergii, considering the effect of the 4 factors microwave irradiation time, microwave power, solid to water ratio and ultrasonic pretreatment time by single factor experiment. In addition, the...

  • Fast and continuous plasma extraction from whole human blood based on expanding cell-free layer devices. Sollier, Elodie; Cubizolles, Myriam; Fouillet, Yves; Achard, Jean-Luc // Biomedical Microdevices;Jun2010, Vol. 12 Issue 3, p485 

    This paper presents promising microfluidic devices designed for continuous and passive extraction of plasma from whole human blood. These designs are based on red cells lateral migration and the resulting cell-free layer locally expanded by geometric singularities such as an enlargement of the...

  • Microwave-assisted Extraction of Alantolactone and Isoalantolactone from Inula helenium. ZHAO, Y. M.; WANG, J.; LIU, H. B.; GUO, C. Y.; ZHANG, W. M. // Indian Journal of Pharmaceutical Sciences;Jan/Feb2015, Vol. 77 Issue 1, p116 

    Microwave-assisted extraction was used for the extraction of alantolactone and isoalantolactone from Inula helenium. Effects of various experimental factors including ethanol concentration, particle size, microwave radiation time, the ratio of material to liquid and extraction temperature on...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics