Reparative properties of a commercial fish protein hydrolysate preparation

Fitzgerald, A. J.; Rai, P. S.; Marchbank, T.; Taylor, G. W.; Ghosh, S.; Ritz, B. W.; Playford, R. J.
June 2005
Gut;Jun2005, Vol. 54 Issue 6, p775
Academic Journal
Background: A partially hydrolysed and dried product of pacific whiting fish is currently marketed as a health food supplement to support "intestinal health". However, there has been only limited scientific study regarding its true biological activity. Aims: We therefore tested its efficacy in a variety of models of epithelial injury and repair. Methods: Effects on proliferation were determined using [3H] thymidine incorporation into epithelial rat intestinal RIE- 1 and human colonic HT29 cells. Effects on restitution (cell migration) were analysed using wounded HT29 monolayers and its ability to influence gastric injury analysed using a rat indomethacin restraint mode!. Partial characterisation of bioactive agents was performed using mass spectroscopy, high i pressure liquid chromatography, and gas chromatography. Results: Both cell proliferation and cell migration were increased by about threefold when added at 1 mg/mI (p<0.01). Gastric injury was reduced by 59% when gavaged at 25 mg/mI (p<0.05), results similar to using the potent cytoprotective agent epidermal growth factor at 12.5 μg/ml. The vast majority of biological activity was soluble in ethanol, with glutamine in its single, di-, and tripeptide forms probably accounting for approximately 40% of the total bioactivity seen. Fatty acid constituents may also have contributed to cell migratory activity. Conclusions: Fish protein hydrolysate possesses biological activity when analysed in a variety of models of injury and repair and could provide a novel inexpensive approach for the prevention and treatment of the injurious effects of non-steroidal anti-inflammatory drugs and other ulcerative conditions of the bowel. Further studies appear justified.


Related Articles

  • CD151 gene delivery increases eNOS activity and induces ECV304 migration, proliferation and tube formation. Zhen-zhong Zheng; Zheng-xiang Liu // Acta Pharmacologica Sinica;Jan2007, Vol. 28 Issue 1, p66 

    Aim: To investigate the effects of CD151 on the activity of endothelial NO synthase (eNOS), and ECV304 migration, proliferation and tube formation. Methods: pAAV-CD151 and pAAV-anti-CD151 were constructed and used to transiently transfect ECV304 mediated with Lipofectamine 2000. After...

  • Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Blagoev, Blagoy; Ong, Shao-En; Kratchmorova, Irina; Mann, Matthias // Nature Biotechnology;Sep2004, Vol. 22 Issue 9, p1139 

    To study the global dynamics of phosphotyrosine-based signaling events in early growth factor stimulation, we developed a mass spectrometric method that converts temporal changes to differences in peptide isotopic abundance. The proteomes of three cell populations were metabolically encoded with...

  • Profiling of Differentially Expressed Genes in LRRC4 Overexpressed Glioblastoma Cells by cDNA Array. Qiu-Hong Zhang; Ming-Hua Wu; Li-Li Wang; Li Cao; Ke Tang; Cong Peng; Kai Gan; Xiao-Ling Li; Gui-Yuan Li // Acta Biochimica et Biophysica Sinica;Oct2005, Vol. 37 Issue 10, p680 

    Our previous study has shown that LRRC4 is a novel member of the leucine-rich repeat (LRR) superfamily and has the potential to suppress brain tumor growth. In order to further analyze the functions of LRRC4 on the maintenance of normal function and suppression of tumorigenesis in the central...

  • AMINOS&PROTEINS Build a Body Good. Pehanich, Mike // Prepared Foods;Mar2003, Vol. 172 Issue 3, pNS24 

    Focuses on the market share of health products that contain amino acids, peptides and protein from diverse sources. Functionality of amino acids; Popularity of foods containing amino acids among athletes; Companies providing various health foods containing amino acids. INSET: Going Global.

  • Protein Quality of Stingray (Himantura gerrardi) Fish Flakes. Mardiah, Ainul; Huda, Nurul; Ahmad, Ruzita // Journal of Fisheries & Aquatic Science;2012, Vol. 7 Issue 6, p485 

    The objectives of this research were to determine the protein quality of fish flakes made from stingray meat (Himantura gerrardi) and to evaluate the changes of the protein quality; Amino Acid Composition (AAC), Chemical Score (CS), Amino Acid Score (AAS) and Essential Amino Acid Index (EAAI)...

  • Production of Enzymatic Protein Hydrolysates from Freshwater Catfish (Clarias batrachus). Md Seniman, Maizatul Sarah; Yusop, Salma Mohamad; Babji, Abdul Salam // AIP Conference Proceedings;2014, Vol. 1614, p323 

    Fish protein hydrolysate (FPH) was prepared from freshwater catfish (Clarias batrachus) by using Alcalase® 2.4L and Papain. The effect of hydrolysis time (30, 60, 120, 180 min) with enzyme concentration of 1% (v/w substrate); pH = 8.0, 7.0 was studied to determine the degree of hydrolysis...

  • Fish protein hydrolysis by a psychrotrophic marine bacterium isolated from the gut of hake (Merluccius hubbsi). Belchior, Silvia G. Estevao; Vacca, Gabriela // Canadian Journal of Microbiology;Dec2006, Vol. 52 Issue 12, p1266 

    The aims of this study were to identify a psychrotrophic bacterium, strain CR41, producing a cold adapted protease during growth at low temperatures and to evaluate the ability of the cells to hydrolyze hake fish protein. The strain was isolated from the intestinal tract of hake collected from...

  • Advances in bioseparations for food and bioprocessing. Ottens, Marcel; Fee, Conan // Food & Bioproducts Processing: Transactions of the Institution o;Apr2014, Vol. 92 Issue 2, p99 

    An introduction to the journal is presented in which the editor discusses various reports within the issue on topics including nanofiltration in the food industry, beneficial health food components, and stimuli-responsive polymers in ion exchange chromatography.

  • Arrest Defective-1 Controls Tumor Cell Behavior by Acetylating Myosin Light Chain Kinase. Dong Hoon Shin; Yang-Sook Chun; Kyoung-Hwa Lee; Hyun-Woo Shin; Jong-Wan Park // PLoS ONE;2009, Vol. 4 Issue 10, p1 

    Background: The enhancement of cell motility is a critical event during tumor cell spreading. Since myosin light chain kinase (MLCK) regulates cell behavior, it is regarded as a promising target in terms of preventing tumor invasion and metastasis. Since MLCK was identified to be associated with...


Read the Article


Sign out of this library

Other Topics