TITLE

Reparative properties of a commercial fish protein hydrolysate preparation

AUTHOR(S)
Fitzgerald, A. J.; Rai, P. S.; Marchbank, T.; Taylor, G. W.; Ghosh, S.; Ritz, B. W.; Playford, R. J.
PUB. DATE
June 2005
SOURCE
Gut;Jun2005, Vol. 54 Issue 6, p775
SOURCE TYPE
Academic Journal
DOC. TYPE
Editorial
ABSTRACT
Background: A partially hydrolysed and dried product of pacific whiting fish is currently marketed as a health food supplement to support "intestinal health". However, there has been only limited scientific study regarding its true biological activity. Aims: We therefore tested its efficacy in a variety of models of epithelial injury and repair. Methods: Effects on proliferation were determined using [3H] thymidine incorporation into epithelial rat intestinal RIE- 1 and human colonic HT29 cells. Effects on restitution (cell migration) were analysed using wounded HT29 monolayers and its ability to influence gastric injury analysed using a rat indomethacin restraint mode!. Partial characterisation of bioactive agents was performed using mass spectroscopy, high i pressure liquid chromatography, and gas chromatography. Results: Both cell proliferation and cell migration were increased by about threefold when added at 1 mg/mI (p<0.01). Gastric injury was reduced by 59% when gavaged at 25 mg/mI (p<0.05), results similar to using the potent cytoprotective agent epidermal growth factor at 12.5 μg/ml. The vast majority of biological activity was soluble in ethanol, with glutamine in its single, di-, and tripeptide forms probably accounting for approximately 40% of the total bioactivity seen. Fatty acid constituents may also have contributed to cell migratory activity. Conclusions: Fish protein hydrolysate possesses biological activity when analysed in a variety of models of injury and repair and could provide a novel inexpensive approach for the prevention and treatment of the injurious effects of non-steroidal anti-inflammatory drugs and other ulcerative conditions of the bowel. Further studies appear justified.
ACCESSION #
17176775

 

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