TITLE

ISOLATION AND SCREENING OF MARINE MICROALGAE CHLORELLA SP. _PR1 FOR ANTICANCER ACTIVITY

AUTHOR(S)
GUPTA, PRATIBHA; SINHA, DONA; BANDOPADHYAY, RAJIB
PUB. DATE
December 2014
SOURCE
International Journal of Pharmacy & Pharmaceutical Sciences;2014, Vol. 6 Issue 10, p517
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: The objective of the present study includes isolation, characterization and screening of anticancerous activity against B16F10 cell line using isolated marine microalgae Chlorella sp._PR1. Methods: In this study, marine microalgae Chlorella sp.-PR1 isolated and cultured using f/2 medium and anticancerous activity was assayed using MTT assay. Results: The DMSO extract of Chlorella sp.-PR1 was exhibit anticancerous activity against murine melanoma B16F10 cell line. The extract exhibit reduction of cell viability up to 56% with 2μg/ml concentration. IC50 were calculated and was found that Chlorella sp.-PR1 need 5.5 μg/ml of the compounds to reduce the murine melanoma B16F10 cell viability by 50%. Fluorescence activated cell sorting (FACS) analysis revealed that Chlorella sp.-PR1 extract (8 μg/ml) brought significant inhibition (p<0.01) of the G0-G1 and the S phase. The extract did not seem to affect the G2 -M phase. Conclusion: DMSO extract of Chlorella sp._PR1 (5.5 μg/ml) was found to be potent against murine melanoma B16F10 cell line.
ACCESSION #
99589381

 

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