Analysis of -819 IL-10 gene polymorphism in Syrian patients with aggressive periodontitis

Taleb, Mouhammad Morad; Soukkarieh, Chadi; Sulaiman, Ali Abou
October 2014
International Journal of PharmTech Research;Oct/Nov2014, Vol. 6 Issue 6, p1810
Academic Journal
Periodontitis is a multifactorial disease and its severe forms, such as aggressive periodontitis, are suggested to have a genetic basis. Among the genetic factors, polymorphisms in cytokine genes have recently been described in susceptibility to periodontitis. Interleukin-10 (IL-10) is a multi-functional cytokine thought to play a role in the pathogenesis of periodontitis. A substitution C/T polymorphism in the promoter region of the IL-10 gene at position -819 has been associated with different amounts of IL-10 production. The aim of the present study was to investigate the possible links between -819C/Tpolymorphism of the IL-10 gene in patients with aggressive periodontitis incomparison with periodontitis-free controls. This study included 83 Syrian subjects suffering from aggressive periodontitis referred to American Academy of Periodontology. They were compared to 79 age matched healthy controls. DNA was isolated from peripheral blood cells and genotyping was performed by means of the amplification refractory mutation system polymerase chain reaction (ARMS-PCR) method. Data were analyzedusing the chi-squared test. There was no marked difference in genotype frequencies between the controls and aggressive periodontitis patients (p>0.05). Moreover, neither marked difference was found in their allele frequency nor in allele carriage(p>0.05).These findings suggest that the investigated polymorphism is not associated with periodontal disease in this studied sample of Syrian population.


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