MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer

Weidong Lu; Zhen Xu; Min Zhang; Yun Zuo
November 2014
International Journal of Clinical & Experimental Medicine;2014, Vol. 7 Issue 10, p7286
Academic Journal
It has been reported that miR-19a was up-regulated in gastric cancer (GC), playing an oncogenic role. However, the underlying mechanism is still unknown. Therefore, in our present study, we investigated the role of miR-19a in gastric tissues as well as 2 GC cell lines. In vivo in clinical tissue level, we have detected basal expression level of miR-19a using real-time reversal transcriptional PCR (RT-PCR); in addition, the relevance between expression of miR-19a and clinic-pathological information was also analyzed. In vitro in cell line level, miR-19a was ectopically expressed using over expression and knock-down strategy. It was found that the overexpression of miR- 19a was significantly associated with metastasis of GC and inferior overall prognosis on clinical tissue level; that promotes the proliferation, migration and invasion; and that overexpression of miR-19a can promote the epithelial-mesenchymal transition through activating PI3K/AKT pathway. Blocking the PI3K/AKT pathway could cancel the effect of miR-19a. All together, our results suggest that miR-19a could be used as a promising therapeutic target in the treatment of GC.


Related Articles

  • miR-141 suppresses proliferation and motility of gastric cancer cells by targeting HDGF. Chen, Bitao; Huang, Tao; Jiang, Jun; Lv, Lei; Li, Hongxing; Xia, Shitao // Molecular & Cellular Biochemistry;Mar2014, Vol. 388 Issue 1/2, p211 

    miR-141 belongs to the miR-200 family, and has been found to be associated with numerous human malignancies; however, its role in gastric cancer (GC) has not been examined in detail. Here, we validated that miR-141 was decreased in GC tissues and cell lines. Forced expression of miR-141...

  • The Interaction Between MiR-141 and lncRNA-H19 in Regulating Cell Proliferation and Migration in Gastric Cancer. Zhou, Xiaoying; Ye, Feng; Yin, Chengqiang; Zhuang, Ya; Yue, Ge; Zhang, Guoxin // Cellular Physiology & Biochemistry (Karger AG);Jul2015, Vol. 36 Issue 4, p1440 

    Background/Aims: Non-coding RNAs including miRNA and lncRNA had been reported to regulate gene expression and were both related to cancer progression. MicroRNA-141 (miR-141) has been reported to play a role in the epithelial to mesenchymal transition (EMT) process and H19 has also been...

  • Snail-Regulated MiR-375 Inhibits Migration and Invasion of Gastric Cancer Cells by Targeting JAK2. Xu, Yanjun; Jin, Juan; Liu, Yiman; Huang, Zhenxia; Deng, Yujie; You, Tao; Zhou, Tianhua; Si, Jianmin; Zhuo, Wei // PLoS ONE;Jul2014, Vol. 9 Issue 7, p1 

    MicroRNAs (miRNAs) have been reported to play a critical role in cancer invasion and metastasis. Our previous study showed that miR-375 frequently downregulated in gastric cancer suppresses cell proliferation by targeting Janus kinase 2 (JAK2). Here, we further found that the expression level of...

  • miR-196b regulates gastric cancer cell proliferation and invasion via PI3K/AKT/mTOR signaling pathway. NA LI; WEIQIANG WANG; BIN XU; HONGYUN GONG // Oncology Letters;Mar2016, Vol. 11 Issue 3, p1745 

    miR-196b plays a significant role in the regulation of tumor pathogenesis and progression by promoting tumor cell proliferation, invasion and metastasis. In order to explore the effects of miR-196b on the proliferation and invasion ability of gastric cancer cells and the involved mechanisms, in...

  • Effects of MicroRNA-206 on Osteosarcoma Cell Proliferation, Apoptosis, Migration and Invasion by Targeting ANXA2 Through the AKT Signaling Pathway. Bao-Long Pan; Zong-Wu Tong; Ling Wu; Li Pan; Jun-E Li; You-Guang Huang; Shu-De Li; Shi-Xun Du; Xu-Dong Li // Cellular Physiology & Biochemistry (Karger AG);Mar2018, Vol. 45 Issue 4, p1410 

    Background/Aims: This study aimed to investigate the mechanism by which microRNA-206 (miR-206) affects the proliferation, apoptosis, migration and invasion of osteosarcoma (OS) cells by targeting ANXA2 via the AKT signaling pathway. Methods: A total of 132 OS tissues and 120 osteochondroma...

  • MicroRNA-302a Suppresses Tumor Cell Proliferation by Inhibiting AKT in Prostate Cancer. Zhang, Gui-Ming; Bao, Chun-Yang; Wan, Fang-Ning; Cao, Da-Long; Qin, Xiao-Jian; Zhang, Hai-Liang; Zhu, Yao; Dai, Bo; Shi, Guo-Hai; Ye, Ding-Wei // PLoS ONE;Apr2015, Vol. 10 Issue 4, p1 

    Micro (mi) RNAs are important regulators involved in various physical and pathological processes, including cancer. The miRNA-302 family has been documented as playing a critical role in carcinogenesis. In this study, we investigated the role of miRNA-302a in prostate cancer (PCa). MiRNA-302a...

  • Tumor suppressor miR-24 restrains gastric cancer progression by downregulating RegIV. Yantao Duan; Lei Hu; Bing Liu; Beiqin Yu; Jianfang Li; Min Yan; Yingyan Yu; Chen Li; Liping Su; Zhenggang Zhu; Ming Xiang; Bingya Liu; Qiumeng Yang // Molecular Cancer;2014, Vol. 13 Issue 1, p1 

    Background microRNAs are small noncoding RNAs that modulate a variety of cellular processes by regulating multiple targets, which can promote or inhibit the development of malignant behaviors. Accumulating evidence suggests miR-24 plays important roles in human carcinogenesis. However, its...

  • microRNA-133b downregulation and inhibition of cell proliferation, migration and invasion by targeting matrix metallopeptidase-9 in renal cell carcinoma. DEYAO WU; HUIXING PAN; YUNFENG ZHOU; JIAN ZHOU; YUANFENG FAN; PING QU // Molecular Medicine Reports;2014, Vol. 9 Issue 6, p2491 

    microRNA (miRNA)-133b has been revealed to be downregulated in head and neck/oral, bladder, human non-small cell lung, colorectal and esophageal squamous cell cancer. The present study examined the expression of miR-133b in renal cell carcinoma (RCC) cell lines and the effects of miRNA-133b on...

  • Cystatin M抑制胃癌SGC-7901细胞的增殖和迁移. 赵亚敏; 何炜; 王峰; 赵国强; 叶延伟; 樊青霞 // Journal of Xi'an Jiaotong University (Medical Sciences);May2014, Vol. 35 Issue 3, p1 

    Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector, and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells. Methods There were three...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics