Enzymatic hydrolysis of market vegetable waste and subsequent ethanol fermentation-Kinetic evaluation

Chiranjeevi, T.; Uma, A.; Radhika, K.; Baby Rani, G.; Prakasham, R. S.; Srinivasa Rao, P.; Umakanth, A. V.
July 2014
Journal of Biochemical Technology;2014, Vol. 5 Issue 4, p775
Academic Journal
In this work, kinetic properties evaluation was made for bioethanol production from sugar hydrolysate of vegetable waste. The saccharified sugars were fermented by using Saccharomyces cerevisiae. The effect of various saccharification factors on sugars release were studied and observed that the optimized conditions contributed to 14.4 gL-1of fermentable sugars production. The produced sugars were subjected to batch fermentation by Saccharomyces cerevisiae at pH 4.5 and the kinetic parameters of fermentation were estimated by fitting the experimental data in modified logistic equations. The data revealed product (ethanol) yield (YP/S) of 0.39g/g of reducing sugars. Maximum specific growth rate (μmax), the yield of ethanol on biomass (YP/X) and the yield of biomass on sugars utilization (YX/S) were determined to be 0.18 h-1, 1.097 g/g and 0.313 g/g, respectively. The process yielded 4.13 gL-1 of ethanol by consumption of 10.6 gL-1 sugars with a volumetric production rate of 0.0861±0.002 gL-1 h-1.


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