Profile of MicroRNA Expression in Brain Tumors of Different Malignancy

Koshkin, F.; Chistyakov, D.; Nikitin, A.; Konovalov, A.; Potapov, A.; Usachyov, D.; Pitskhelauri, D.; Kobyakov, G.; Shishkina, L.; Chekhonin, V.
October 2014
Bulletin of Experimental Biology & Medicine;Oct2014, Vol. 157 Issue 6, p794
Academic Journal
The expression profiles of 10 mature microRNA (7, 10a, 17, 20a, 21, 23a, 26a, 137, 222) in biopsy specimens of gliomas of different malignancy were studied by the real time PCR with SYBR Green I fluorescent stain. The expression of microRNA-21 increased significantly, while that of microRNA-137 decreased, depending on the tumor malignancy. The expression of microRNA-9, −17, −20a, −23a, −26a was significantly higher, while that of microRNA-7 lower in the tumor vs. control tissue samples. New data on the molecular pathological mechanisms of gliomas related to their malignancy were obtained. Quantitative analysis of microRNA was suggested as a potential diagnostic marker of brain tumors.


Related Articles

  • Water Channels Aquaporin 4 and -1 Expression in Subependymoma Depends on the Localization of the Tumors. Noell, Susan; Fallier-Becker, Petra; Mack, Andreas F.; Hoffmeister, Maike; Beschorner, Rudi; Ritz, Rainer // PLoS ONE;Jun2015, Vol. 10 Issue 6, p1 

    Background: We analyzed aquaporin 4 and -1 expression in subependymomas, benign and slow growing brain tumors WHO grade I. Ten subependymoma cases were investigated, five of the fossa inferior and five of the fossa superior. Methods and Results: Using immunohistochemistry, we observed...

  • MicroRNA Signature in Thyroid Fine Needle Aspiration Cytology Applied to 'Atypia of Undetermined Significance' Cases. Shen, Rulong; Liyanarachchi, Sandya; Li, Wei; Wakely, Paul E.; Saji, Motoyasu; Huang, Jie; Nagy, Rebecca; Farrell, Tisha; Ringel, Matthew D.; de la Chapelle, Albert; Kloos, Richard T.; He, Huiling // Thyroid;Jan2012, Vol. 22 Issue 1, p9 

    Background: MicroRNA (miR) expression signatures are proposed to be able to differentiate thyroid cancer from benign thyroid lesions. We selected eight miRs (miR-146b, -221, -187, -197, -346, -30d, -138, and -302c) to examine the potential use of miRs to supplement diagnostic cytology in cases...

  • Classification of Subpopulations of Cells Within Human Primary Brain Tumors by Single Cell Gene Expression Profiling. Möllerström, Elin; Rydenhag, Bertil; Andersson, Daniel; Lebkuechner, Isabell; Puschmann, Till; Chen, Meng; Wilhelmsson, Ulrika; Ståhlberg, Anders; Malmgren, Kristina; Pekny, Milos // Neurochemical Research;Feb2015, Vol. 40 Issue 2, p336 

    Brain tumors are heterogeneous with respect to genetic and histological properties of cells within the tumor tissue. To study subpopulations of cells, we developed a protocol for obtaining viable single cells from freshly isolated human brain tissue for single cell gene expression profiling. We...

  • MicroRNA profile: a promising ancillary tool for accurate renal cell tumour diagnosis. Silva-Santos, R M; Costa-Pinheiro, P; Luis, A; Antunes, L; Lobo, F; Oliveira, J; Henrique, R; Jerónimo, C // British Journal of Cancer;11/12/2013, Vol. 109 Issue 10, p2646 

    Background:Renal cell tumours (RCTs) are clinically, morphologically and genetically heterogeneous. Accurate identification of renal cell carcinomas (RCCs) and its discrimination from normal tissue and benign tumours is mandatory. We, thus, aimed to define a panel of microRNAs that might aid in...

  • A Micro RNA Processing Defect in Rapidly Progressing Idiopathic Pulmonary Fibrosis. Oak, Sameer R.; Murray, Lynne; Herath, Athula; Sleeman, Matthew; Anderson, Ian; Joshi, Amrita D.; Coelho, Ana Lucia; Flaherty, Kevin R.; Toews, Galen B.; Knight, Darryl; Martinez, Fernando J.; Hogaboam, Cory M. // PLoS ONE;2011, Vol. 6 Issue 6, p1 

    Background: Idiopathic pulmonary fibrosis exhibits differential progression from the time of diagnosis but the molecular basis for varying progression rates is poorly understood. The aim of the present study was to ascertain whether differential miRNA expression might provide one explanation for...

  • Self-Construal Priming Affects Speed of Retrieval from Short-Term Memory. MacDonald, Justin A.; Sandry, Joshua; Rice, Stephen // PLoS ONE;Nov2012, Vol. 7 Issue 11, Special section p1 

    We investigated the effects of collective or individual self-construal priming on recall in a short-term memory (STM) task. We primed participants to either their individual or their collective self-construals or a neutral control condition. Participants then completed a STM retrieval task using...

  • Association Between pri-miR-218 Polymorphism and Risk of Hepatocellular Carcinoma in a Han Chinese Population. Zhang, Lu-Shun; Liang, Wei-Bo; Gao, Lin-Bo; Li, Hong-Yu; Li, Li-Juan; Chen, Peng-Yu; Liu, Yun; Chen, Tian-Yi; Han, Jun-Ge; Wei, Yong-Gang; Zhang, Lin // DNA & Cell Biology;May2012, Vol. 31 Issue 5, p761 

    MicroRNAs are noncoding RNA molecules of 18-25 nucleotides that regulate gene expression at the post-transcriptional level. The aim of this study was to investigate whether pri-miR-218 rs11134527 A/G polymorphism influences the risk of hepatocellular carcinoma (HCC) or not. pri-miR-218...

  • Identification of suitable reference genes for hepatic microRNA quantitation. Lamba, Vishal; Ghodke-Puranik, Yogita; Weihua Guan; Lamba, Jatinder K. // BMC Research Notes;2014, Vol. 7 Issue 1, p1 

    Background MicroRNAs (miRNAs) are short (~22 nt) endogenous RNAs that play important roles in regulating expression of a wide variety of genes involved in different cellular processes. Alterations in microRNA expression patterns have been associated with a number of human diseases. Accurate...

  • Comparative Characterization of MicroRNAs from the Liver Flukes Fasciola gigantica and F. hepatica. Min-Jun Xu; Lin Ai; Jing-Hua Fu; Nisbet, Alasdair J.; Qing-You Liu; Mu-Xin Chen; Dong-Hui Zhou; Xing-Quan Zhu // PLoS ONE;Dec2012, Vol. 7 Issue 12, p1 

    MicroRNAs (miRNAs) are key regulators of gene expression at the post-transcription level. The present study specifically explored and compared the miRNA expression profiles of F. gigantica and F. hepatica using an integrated sequencing and bioinformatics platform and quantitative real-time PCR....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics