TITLE

SIGNIFICANCE OF ANTIOXIDANT ENZYMES IN STRESS SIGNALING

AUTHOR(S)
Naz, Sabiha; Pandey, Bhawana; Shrivastava, Awadhesh Kumar
PUB. DATE
August 2012
SOURCE
Advances in Pharmacology & Toxicology;Aug2012, Vol. 13 Issue 2, p47
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Plant expose to stress undergo changes in their metabolism in order to adapt to the changes in their environment. The present study was conducted to reveal the role of antioxidant enzymes like superoxide dismutase and peroxidase during acetyl salicylic acid and NaCl signaling in W. sominifera (L.) Dunal. using enzyme activity staining on acrylamide gels. The results showed that during acetyl salicylic acid signaling, SOD activity increased in 2nd & 4th hrs and decreased till 6th hrs and further increased up to 10th hrs. Similarly in peroxidase, a low enzyme activity was observed in 0 and 6th hour while in 2nd, 4th, 8th and 10th hour the expressions of three isozymes of peroxidases were observed. Activity by 6th hour may result in increased accumulation of H2O2 resulting in oxidative burst. The decrease in SOD activity may be due to the feedback inhibition mechanism of the enzyme. During NaCl stress only SOD activity was localized since POD activity was negligible. In different NaCl concentration from 1000 to 6000 ppm, the SOD activity was maximum increase during 5000 ppm treatment revealed by the presence of three bands. During time dependent NaCl treatment, the SOD activity increased during 2nd to 8th hour of treatment revealed by the presence of two bands. The result in the present study highlight a probable occurrence of oxidative burst in Withania sominifera (L.) during 4th and 6th hours of stress signaling. Sometimes high amount of H2O2 accumulated and generating PCR/HR signals against plant pathogens to protect plant.
ACCESSION #
98885107

 

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