Optimization of Chromatographic Criterion to Recover Indian White Shrimp (Fenneropenaeus indicus) Alkaline Phosphatase from Hepatopancreas

Nooralabettu, Krishna Prasad
September 2014
British Biotechnology Journal;2014, Vol. 4 Issue 9, p1011
Academic Journal
Commercially important alkaline phosphatase can be recovered from the hepatopancreatic tissue of Indian white shrimp with optimum yield, activity, and purity by carefully designing the criterions of ion exchange chromatographic purification. The tissues were homogenized, clarified, and concentrated. Concentrated homogenate was purified with optimum yield, activity and purity by optimising criterions of chromatography such as pH and ionic strength of the binding buffer, and ionic gradient and the flow rate of the elution buffer in DEAE-cellulose column. The enzyme was optimally bound to the column at pH 8.4 and ionic strength 0.1 M NaCl at flow rate of 1mL/min, and eluted with high resolution at ionic gradient of 0.10-0.35 M NaCl in 25 min at a flow rate of 1.5mL/min, and hence is the optimum criterions of chromatographic purification.


Related Articles

  • Recovery of Sinapic Acid from the Waste Effluent of Mustard Protein Isolation by Ion Exchange Chromatography. Prapakornwiriya, N.; Diosady, L. // Journal of the American Oil Chemists' Society (JAOCS);Feb2014, Vol. 91 Issue 2, p357 

    Sinapic acid present in the waste stream of yellow mustard protein isolation was purified by strong base Dowex (1 × 8, Cl) ion exchange chromatography. The ratio of loading volume to resin bed volume was 19:1. Approximately 80 % of sinapic acid was adsorbed. The column was washed with two...

  • Determining Carbohydrates in Essential and Nonessential Foodstuffs using Ion Chromatography. Steinbach, Alfred; Wille, Andrea // LC-GC Asia Pacific;Nov2010, Vol. 13 Issue 4, p1 

    This article describes a straightforward ion chromatographic method that uses isocratic elution and pulsed amperometric detection (PAD) to sensitively determine water-soluble polyols and sugar alcohols as well as mono, di- and oligosaccharides in essential and non-essential foodstuffs. While...

  • Open capillary columns for microscale HPLC. Gursky, V. S.; Kharitonova, E. Yu. // Vestnik Sankt-Peterburgskogo universiteta, Seriia 7: Geologia, G;2013, Issue 2, p162 

    For the development of capillary version of ion chromatography a new approach to manufacture open capillary chromatography columns was suggested. The production of columns of inner diameter 60 μm and length 1000 mm from hollow fiber TF-4SK (OS «Plastpolymer», Saint-Petersburg) was...

  • Purification and characterization of alkaline phosphatase from the gut of sea cucumber Stichopus japonicus. Wu, Hai-Tao; Li, Dong-Mei; Zhu, Bei-Wei; Cheng, Jing-Heng; Sun, Jin-Jian; Wang, Feng-Lin; Yang, Yang; Song, Yu-Kun; Yu, Chen-Xu // Fisheries Science;May2013, Vol. 79 Issue 3, p477 

    An alkaline phosphatase was purified from the gut of sea cucumber Stichopus japonicus by n-butyl alcohol extract, ammonium sulfate precipitation, ion exchange chromatography with diethylaminoethyl cellulose, gel filtration chromatography with Sephacryl S-200 and preparative electrophoresis with...

  • Erratum to: Response to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei. Andriantahina, Farafidy; Liu, Xiaolin; Huang, Hao; Xiang, Jianhai // Chinese Journal of Oceanology & Limnology;May2012, Vol. 30 Issue 3, p506 

    A correction to the article "Response to selection, heritability and genetic correlations between body weight and body size in Pacific white shrimp, Litopenaeus vannamei," that appeared in the 2012 issue is presented.

  • Analysis of an Herbicide Using Anion-Exchange LC with Indirect Conductivity Detection.  // LC-GC North America;02/01/2002 Supplement, Vol. 20 Issue 2, p25 

    Examines the use of the PRP-X100 trimethylammonium anion-exchange column with indirect conductivity detection for the analysis of the herbicide glufosinate. Advantages of the separation method; Comparison of sensitivity for varying injection volumes.

  • Sialylation Levels Influence Oligosaccharide Quantitation. Ganesa, Chandrashekar; Granda, Brian W.; Mattaliano, Robert J. // BioPharm International;Jun2003, Vol. 16 Issue 6, p44 

    Analyzes the response variability among detection cells when using high-pH anion-exchange chromatography with pulsed amperometric detection technology for oligosaccharide characterization. Influence of sialylation levels on oligosaccharide quantitation; Advantage of the method; Structures of...

  • Analysis of anions in beer using ion chromatography. Bruce, Jonathan // Journal of Automated Methods & Management in Chemistry;Jul2002, Vol. 24 Issue 4, p127 

    The majority of anions found in beer are a consequence of impurities derived from the water used during the brewing process. The process of beer manufacture consists of malting, brewing and fermentation followed by maturation before filtration and finally storage. Strict quality control is...

  • Cation-Exchange Separation of Proteins on a Bonded Hydrophilic Polymer-Coated Silica Support.  // LC-GC North America;02/01/2002 Supplement, Vol. 20 Issue 2, p17 

    Examines the use of the low-capacity ion exchanger BioBasic� SCX column for separating proteins and peptides with low salt concentrations. Information on ion-exchange chromatography; Characteristics of the BioBasic SCX packing; Use of the column in protein chromatography.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics