TITLE

A Comparative Study of Osteogenic Differentiation Human Induced Pluripotent Stem Cells and Adipose Tissue Derived Mesenchymal Stem Cells

AUTHOR(S)
Ardeshirylajimi, Abdolreza; Soleimani, Masoud; Hosseinkhani, Saman; Parivar, Kazem; Yaghmaei, Parichehr
PUB. DATE
September 2014
SOURCE
Cell Journal (Yakhteh);Autumn2014, Vol. 16 Issue 3, p235
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: Human induced pluripotent stem cells (iPSCs) have been shown to have promising capacity for stem cell therapy and tissue engineering applications. Therefore, it is essential to compare the ability of these cells with the commonly used mesenchymal stem cells (MSC) for bone tissue engineering in vitro. Materials and Methods: In this experimental study, the biological behavior and osteogenic capacity of the iPSCs were compared with MSCs isolated from human adipose tissue (AT-MSCs) using 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Alizarin red staining, alkaline phosphatase (ALP) activity measurements, calcium content assay and common osteogenic-related genes. Data were reported as the mean ± SD. One-way analysis of variance (ANOVA) was used to compare the results. A p value of less than 0.05 was considered statistically significant. Results: There was a significant difference between the rate of proliferation of the two types of stem cells; iPSCs showed increased proliferation compared to AT-MSCs. During osteogenic differentiation, ALP activity and mineralization were demonstrated to be significantly higher in iPSCs. Although AT-MSCs expressed higher levels of Runx2, iPSCs expressed higher levels of osteonection and osteocalcin during differentiation. Conclusion: iPSCs showed a higher capacity for osteogenic differentiation and hold promising potential for bone tissue engineering and cell therapy applications.
ACCESSION #
98850488

 

Related Articles

  • Dedifferentiated adipocyte-derived progeny cells (DFAT cells): Potential stem cells of adipose tissue. Wei, Shengjuan; Zan, Linsen; Hausman, Gary J; Rasmussen, Theodore P; Bergen, Werner G; Dodson, Michael V // Adipocyte;Jul/Aug2013, Vol. 2 Issue 3, p1 

    Analyses of mature adipocytes have shown that they possess a reprogramming ability in vitro, which is associated with dedifferentiation. The subsequent dedifferentiated fat cells (DFAT cells) are multipotent and can differentiate into adipocytes and other cell types as well. Mature adipocytes...

  • Prospect of Stem Cells in Bone Tissue Engineering: A Review. Yousefi, Azizeh-Mitra; James, Paul F.; Akbarzadeh, Rosa; Subramanian, Aswati; Flavin, Conor; Oudadesse, Hassane // Stem Cells International;1/6/2016, p1 

    Mesenchymal stem cells (MSCs) have been the subject of many studies in recent years, ranging from basic science that looks into MSCs properties to studies that aim for developing bioengineered tissues and organs. Adult bone marrow-derived mesenchymal stem cells (BM-MSCs) have been the focus of...

  • The Therapeutic Potential, Challenges and Future Clinical Directions of Stem Cells from the Wharton's Jelly of the Human Umbilical Cord. Bongso, Ariff; Fong, Chui-Yee // Stem Cell Reviews & Reports;Apr2013, Vol. 9 Issue 2, p226 

    Mesenchymal stem cells (MSCs) from bone marrow, adult organs and fetuses face the disadvantages of invasive isolation, limited cell numbers and ethical constraints while embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) face the clinical hurdles of potential immunorejection...

  • Influence of novel porous calcium phosphate cement on biological behavior of bone marrow mesenchymal stem cells. Wang Shuhong; Zhang Xiong; Zhang Jin'e; Huang Yuanliang // West China Journal of Stomatology;Oct2012, Vol. 30 Issue 5, p544 

    Objective To investigate the effect of novel porous calcium phosphate cement (CPC) scaffoldings on attachment, proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs of Beagle dogs were implanted and cultured with CPC scaffoldings in intro, tricalcium...

  • In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem Cells. Sakurai, Hidetoshi; Sakaguchi, Yasuko; Shoji, Emi; Nishino, Tokiko; Maki, Izumi; Sakai, Hiroshi; Hanaoka, Kazunori; Kakizuka, Akira; Sehara-Fujisawa, Atsuko // PLoS ONE;Oct2012, Vol. 7 Issue 10, Special section p1 

    Induced pluripotent stem (iPS) cells are generated from adult somatic cells by transduction of defined factors. Given their unlimited proliferation and differentiation potential, iPS cells represent promising sources for cell therapy and tools for research and drug discovery. However, systems...

  • Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells. Galvão Barboza, Carlos Augusto; Ginani, Fernanda; Soares, Diego Moura; Gomes Henriques, Águida Cristina; Almeida Freitas, Roseana de // Einstein (16794508);Jan-Mar2014, Vol. 12 Issue 1, p75 

    Objective: To evaluate the effect of low-level laser irradiation on the proliferation and possible nuclear morphological changes of mouse mesenchymal stem cells. Methods: Mesenchymal stem cells derived from bone marrow and adipose tissue were submitted to two applications (T0 and T48 hours) of...

  • Adipose-Derived Mesenchymal Stromal/Stem Cells: Tissue Localization, Characterization, and Heterogeneity. Baer, Patrick C.; Geiger, Helmut // Stem Cells International;2012, p1 

    Adipose tissue as a stem cell source is ubiquitously available and has several advantages compared to other sources. It is easily accessible in large quantities with minimal invasive harvesting procedure, and isolation of adipose-derived mesenchymal stromal/stem cells (ASCs) yields a high amount...

  • Stem cells in musculoskeletal system for clinical application. Byoung-Hyun Min; Tian Zhu Li // Journal of the Korean Medical Association / Taehan Uisa Hyophoe ;May2011, Vol. 54 Issue 5, p491 

    Mesenchymal stem cells (MSCs) play a crucial role in the proliferation and differentiation of human tissue such as bone, cartilage, muscle, fat, and fibroblasts. Various surgical techniques have been developed for the repair of the musculoskeletal system, but they can be often limited. Thus, the...

  • Manufacturing of Human Umbilical Cord Mesenchymal Stromal Cells on Microcarriers in a Dynamic System for Clinical Use. Petry, Florian; Smith, J. Robert; Leber, Jasmin; Salzig, Denise; Czermak, Peter; Weiss, Mark L. // Stem Cells International;2/8/2016, p1 

    The great properties of human mesenchymal stromal cells (hMSCs) make these cells an important tool in regenerative medicine. Because of the limitations of hMSCs derived from the bone marrow during isolation and expansion, hMSCs derived from the umbilical cord stroma are a great alternative to...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics