TITLE

Associations between Individual and Combined Polymorphisms of the TNF and VEGF Genes and the Embryo Implantation Rate in Patients Undergoing In Vitro Fertilization (IVF) Programs

AUTHOR(S)
Boudjenah, Radia; Molina-Gomes, Denise; Torre, Antoine; Boitrelle, Florence; Taieb, Stéphane; Dos Santos, Esther; Wainer, Robert; de Mazancourt, Philippe; Selva, Jacqueline; Vialard, François
PUB. DATE
September 2014
SOURCE
PLoS ONE;Sep2014, Vol. 9 Issue 9, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: A multiple pregnancy is now considered to be the most common adverse outcome associated with in vitro fertilization (IVF). As a consequence, the identification of women with the best chances of embryo implantation is a challenge in IVF program, in which the objective is to offer elective single-embryo transfer (eSET) without decreasing the pregnancy rate. To date, a range of hormonal and clinical parameters have been used to optimize eSET but none have significant predictive value. This variability could be due to genetic predispositions related to single-nucleotide polymorphisms (SNPs). Here, we assessed the individual and combined impacts of thirteen SNPs that reportedly influence the outcome of in vitro fertilisation (IVF) on the embryo implantation rate for patients undergoing intracytoplasmic sperm injection program (ICSI). Materials and Methods: A 13 gene polymorphisms: FSHR(Asn680Ser), p53(Arg72Pro), AMH(Ile49Ser), ESR2(+1730G>A), ESR1(−397T>C), BMP15(−9C>G), MTHFR1(677C>T), MTHFR2(1298A>C), HLA-G(−725C>G), VEGF(+405G>C), TNFα(−308A>G), AMHR(−482A>G), PAI-1(4G/5G), multiplex PCR assay was designed to genotype women undergoing ICSI program. We analyzed the total patients population (n = 428) and a subgroup with homogeneous characteristics (n = 112). Results: Only the VEGF(+405G>C) and TNFα(−308A>G) polymorphisms impacted fertilization, embryo implantation and pregnancy rates. Moreover, the combined VEGF+405.GG and TNFα-308.AG or AA genotype occurred significantly more frequently in women with high implantation potential. In contrast, the VEGF+405.CC and TNFα-308.GG combination was associated with a low implantation rate. Conclusion: We identified associations between VEGF(+405G>C) and TNFα(−308A>G) polymorphisms (when considered singly or as combinations) and the embryo implantation rate. These associations may be predictive of embryo implantation and could help to define populations in which elective single-embryo transfer should be recommended (or, conversely, ruled out). However, the mechanism underlying the function of these polymorphisms in embryo implantation remains to be determined and the associations observed here must be confirmed in a larger, more heterogeneous cohort.
ACCESSION #
98618444

 

Related Articles

  • The vascular endothelial growth factor ( VEGF) +405 G/C polymorphism and its relationship with recurrent implantation failure in women in an IVF programme with ICSI. Boudjenah, Radia; Molina-Gomes, Denise; Wainer, Robert; Mazancourt, Philippe; Selva, Jacqueline; Vialard, François // Journal of Assisted Reproduction & Genetics;Dec2012, Vol. 29 Issue 12, p1415 

    Purpose: Successful embryo implantation depends on trophoblast proliferation, migration and, lastly, invasion of the endometrium (to anchor the trophoblast to the uterus). This invasion is mediated by locally produced soluble factors. Of these, vascular endothelial growth factor (VEGF) is the...

  • Polymorphism of apoptosis regulatory and growth factor genes in patients with chronic lymphocytic leukemia. Bakirov, Bulat A.; Karimov, D. O.; Viktorova, T. V. // Russian Open Medical Journal;2012, Issue 1, p1 

    The objective of the research is to study the role of polymorphic gene variants of Tumor Necrosis Factor-α (TNFA), Bcl-2-associated X protein (BAX), p53 Binding Protein (MDM2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in the development of chronic...

  • Vascular endothelial growth factor expression by hyalocytes and its regulation by glucocorticoid. Hata, Y.; Sassa, Y.; Kita, T.; Miura, M.; Kano, K.; Kawahara, S.; Arita, R.; Nakao, S.; Shih, J. L.; Ishibashi, T. // British Journal of Ophthalmology;Nov2008, Vol. 92 Issue 11, p1540 

    Aim: Tumour necrosis factor-α (TNF-α) is one of the major inflammatory cytokines involved in the pathogenesis of various vitreoretinal diseases. The authors investigated the effect of hypoxia, TNF-α and dexamethasone on vascular endothelial growth factor (VEGF) expression by cultured...

  • Novel pathway compendium analysis elucidates mechanism of pro-angiogenic synthetic small molecule. Kristen A. Wieghaus; Erwin P. Gianchandani; Mikell A. Paige; Milton L. Brown; Edward A. Botchwey; Jason A. Papin // Bioinformatics;Oct2008, Vol. 24 Issue 20, p2384 

    Motivation: Computational techniques have been applied to experimental datasets to identify drug mode-of-action. A shortcoming of existing approaches is the requirement of large reference databases of compound expression profiles. Here, we developed a new pathway-based compendium analysis that...

  • Reduced levels of VEGF-A and MMP-2 and MMP-9 activity and increased TNF-α in menstrual endometrium and effluent in women with menorrhagia.  // Human Reproduction;Aug2006, Vol. 21 Issue 8, p2158 

    BACKGROUND: Heavy regular menstrual periods (menorrhagia) are an important cause of ill health in women and remain the leading indication for hysterectomy. Abnormalities of the endometrial blood vessels are among the possible causes of this condition. Many different factors affect endothelial...

  • Vascular endothelial growth factor gene +405 C/G polymorphism is associated with susceptibility to advanced stage endometriosis. Sung Hoon Kim; Young Min Choi; Seon Ha Choung; Jong Kwan Jun; Jung Gu Kim; Shin Yong Moon // Human Reproduction;Oct2005, Vol. 20 Issue 10, p2904 

    BACKGROUND: Vascular endothelial growth factor (VEGF) is known to play a pivotal role in the development of endometriosis. This study was performed to investigate whether the VEGF gene 5’-untranslated region polymorphism is associated with susceptibility to advanced stage endometriosis....

  • VEGF in Human Uterine Fluid is a Key Regulator. Hannan, Natalie J. // Fertility Weekly;3/26/2012, p8 

    The article reports that according to a study in Australia, vascular endothelial growth factor (VEGF) in uterine fluid helps in embryo implantation. The study, conducted by researcher N.J. Hannan and colleagues, investigated changes in human endometrial secretion and its association with...

  • Vascular endothelial growth factor levels in serum and plasma from patients undergoing controlled ovarian hyperstimulation for IVF. Dolors Manau; Francisco Fábregues; Joana Peñarrubia; Montserrat Creus; Francisco Carmona; Gemma Casals; Wladimiro Jiménez; Juan Balasch // Human Reproduction;Mar2007, Vol. 22 Issue 3, p669 

    BACKGROUND: Vascular endothelial growth factor (VEGF) has been investigated as a marker of ovarian response to controlled ovarian hyperstimulation and as a predictor of ovarian hyperstimulation syndrome (OHSS) in IVF cycles. In most studies, serum has been used for circulating VEGF concentration...

  • Early pregnancy: basic research.  // Human Reproduction;Aug2007, Vol. 22 Issue 0, pi174 

    The article presents abstracts on early pregnancy topics which include the characterisation of lectins in pregnant mouse, the role of vascular endothelial growth factors (VEGFR) in angiogenesis, and effects of methylenetetrahydrofolate reductase gene polymorphisms on miscarriages.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics