TITLE

Role of wheat trHb in nitric oxide scavenging

AUTHOR(S)
Kim, Dae; Hong, Min; Seo, Yong
PUB. DATE
September 2014
SOURCE
Molecular Biology Reports;Sep2014, Vol. 41 Issue 9, p5931
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We examined the role of wheat truncated-hemoglobin ( TatrHb) in nitric oxide (NO) scavenging in transgenic Arabidopsis plants by assessing the response to an NO donor/scavenger and salt stress. The degree of increase in Na and decrease in K levels in the transgenic plants were more than those in the wild-type plants, and the ratio of Na to K increased in the transgenic plants under salt stress. Endogenous NO increased dramatically in the salt-treated wild-type plants but not in the transgenic plants. Additionally, the maximum photosystem II quantum ratio of variable to maximum fluorescence (Fv/Fm) in transgenic plants decreased more significantly than that in the wild-type plants, indicating that the transgenic plants suffered more severe photosynthetic damage because of salt stress than that by the wild type. Similar results were observed in germination experiments by using Murashige and Skoog media containing 100 mM sodium chloride. The Fv/Fm decreased in the leaves of salt-treated transgenic plants, indicating that transgenic seeds were more sensitive to salt stress than that by the wild-type seeds. In addition, the negative effect on seed germination was more severe in transgenic plants than in the wild types under NaCl treatment conditions. The results support the hypothesis that plant trHb shares NO scavenging functions and characteristics with bacterial trHb.
ACCESSION #
97623377

 

Related Articles

  • Seed-specific elevation of non-symbiotic hemoglobin AtHb1: beneficial effects and underlying molecular networks in Arabidopsis thaliana. Thiel, Johannes; Rolletschek, Hardy; Friedel, Svetlana; Lunn, John E.; Nguyen, Thuy H.; Feil, Regina; Tschiersch, Henning; Müller, Martin; Borisjuk, Ljudmilla // BMC Plant Biology;2011, Vol. 11 Issue 1, p48 

    Background: Seed metabolism is dynamically adjusted to oxygen availability. Processes underlying this auto-regulatory mechanism control the metabolic efficiency under changing environmental conditions/stress and thus, are of relevance for biotechnology. Non-symbiotic hemoglobins have been shown...

  • A R2R3-MYB transcription factor gene in common wheat (namely TaMYBsm1) involved in enhancement of drought tolerance in transgenic Arabidopsis. Li, Meng-jun; Qiao, Yu; Li, Ya-qing; Shi, Zhan-liang; Zhang, Nan; Bi, Cai-li; Guo, Jin-kao // Journal of Plant Research;Nov2016, Vol. 129 Issue 6, p1097 

    We isolated the TaMYBsm1 genes, encoding R2R3-type MYB proteins in common wheat, aimed to uncover the possible molecular mechanisms related to drought response. The TaMYBsm1 genes, TaMYBsm1- A, TaMYBsm1- B and TaMYBsm1- D, were isolated and analyzed from the common wheat cultivar Shimai 15....

  • Transgenic wheat now a step closer.  // Nebraska Farmer;Mar2015, Vol. 157 Issue 3, p25 

    The article reports on the developments of an experimental study by scientists from Kansas State University, on generating a transgenic wheat crop which tolerates warmer temperatures in wheat's critical grain filling stage.

  • Functional conservation of wheat orthologs of maize rough sheath1 and rough sheath2 genes. Ryoko Morimoto; Emi Nishioka; Koji Murai; Shigeo Takumi // Plant Molecular Biology;Feb2009, Vol. 69 Issue 3, p273 

    Abstract  Maize rough sheath2 (RS2) and Arabidopsis ASYMMETRIC LEAVES1 (AS1) both encode a Myb transcription factor and repress Knotted1-type homeobox (KNOX) genes. The RS2/AS1-KNOX relationship is functionally conserved between maize and Arabidopsis. Here, we cloned wheat orthologs of...

  • Overexpression of the wheat salt tolerance-related gene TaSC enhances salt tolerance in Arabidopsis. Huang, Xi; Zhang, Yang; Jiao, Bo; Chen, Guiping; Huang, Shenghe; Guo, Feng; Shen, Yinzhu; Huang, 
Zhanjing; Zhao, Baocun // Journal of Experimental Botany;Nov2012, Vol. 63 Issue 15, p5463 

    A novel gene named TaSC was cloned from salt-tolerant wheat. Northern blot showed that the expression of TaSC in salt-tolerant wheat was up-regulated after salt stress. Real-time quantitative PCR analyses showed that TaSC expression was induced by salt and ABA in wheat. Localization analysis...

  • Global transcriptome analysis of AtPAP2 - overexpressing Arabidopsis thaliana with elevated ATP. Feng Sun; Chao Liang; Whelan, James; Jun Yang; Peng Zhang; Boon Leong Lim // BMC Genomics;2013, Vol. 14 Issue 1, p1 

    Background AtPAP2 is a purple acid phosphatase that is targeted to both chloroplasts and mitochondria. Over-expression (OE) lines of AtPAP2 grew faster, produced more seeds, and contained higher leaf sucrose and glucose contents. The present study aimed to determine how high energy status...

  • Overexpressing a Glycogen Synthase Kinase Gene from Wheat, TaGSK1, Enhances Salt Tolerance in Transgenic Arabidopsis. He, Xiaoliang; Tian, Jinghan; Yang, Lixia; Huang, Yanmin; Zhao, Baocun; Zhou, Chunjiang; Ge, Rongchao; Shen, Yinzhu; Huang, Zhanjing // Plant Molecular Biology Reporter;Aug2012, Vol. 30 Issue 4, p807 

    TaGSK1, a salt-inducible gene, has a very high identity with mammalian GSK3 which is a highly conserved serine/threonine protein kinase that regulates glycogen synthesis. Overexpression of TaGSK1 reduces cellular osmotic turgor and cellular Na content and enhances salt tolerance of transgenic...

  • A wheat R2R3-MYB gene, TaMYB30-B, improves drought stress tolerance in transgenic Arabidopsis. Zhang, Lichao; Zhao, Guangyao; Xia, Chuan; Jia, Jizeng; Liu, Xu; Kong, Xiuying // Journal of Experimental Botany;Nov2012, Vol. 63 Issue 16, p5873 

    The MYB-type proteins are involved in various processes of plant growth, development, and stress response. In a previous work, a polyethylene glycol (PEG) stress-induced gene, TaMYB30, which encodes a R2R3-type MYB protein was identified in wheat. In this study, the isolation and functional...

  • Increasing Nitric Oxide Content in Arabidopsis thaliana by Expressing Rat Neuronal Nitric Oxide Synthase Resulted in Enhanced Stress Tolerance. Shi, Hai-Tao; Li, Rong-Jun; Cai, Wei; Liu, Wen; Wang, Chao-Lun; Lu, Ying-Tang // Plant & Cell Physiology;Feb2012, Vol. 53 Issue 2, p344 

    Nitric oxide (NO) plays essential roles in many physiological and developmental processes in plants, including biotic and abiotic stresses, which have adverse effects on agricultural production. However, due to the lack of findings regarding nitric oxide synthase (NOS), many difficulties arise...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics