TITLE

Induction of experimental ulcerative colitis by Fusobacterium varium isolated from colonic mucosa of patients with ulcerative colitis

AUTHOR(S)
Ohkusa, T.; Okayasu, I.; Ogihara, T.; Morita, K.; Ogawa, M.; Sato, N.
PUB. DATE
January 2003
SOURCE
Gut;Jan2003, Vol. 52 Issue 1, p79
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Bacteria are implicated in certain forms of model chronic colitis but the identity and role of bacteria in human ulcerative colitis (UC) are uncertain. Aims: To isolate pathogenic bacteria from inflamed mucosa of patients with UC, to examine whether the bacteria have a toxin to Vero cells, and to determine whether the toxin induces UC-like lesions in animals. Methods: Bacteria were isolated from UC patients and supernatants from cultures were filtered and tested for cytotoxicity to Vero cells. Bacterial cells producing the cytotoxic supernatants were examined by polymerase chain reaction for verotoxin genes. Culture supernatants of cytotoxic strains were examined by high performance liquid chromatography for organic acid concentrations. Mice were given enemas containing organic acid at the mean concentration in the supernatants of cytotoxic strains to ascertain whether colonic lesions appear in UC. Results: Only supernatants from cultures of Fusobacterium varium killed Vero cells. Bacterial cells lacked verotoxin genes. Bacterial culture supernatants contained high concentrations of n-butyric acid and the mean concentration (32 mmol/I) was cytotoxic to Vero cells. Twenty four hours after mice were given enemas containing either butyric acid or F varium culture supernatants, colonic ulcers with crypt abscesses, inflammatory cell infiltration, and apoptotic changes were observed. Conclusions: Butyric acid in culture supernatants from cultures of F varium caused UC-like lesions in mice. This study indicates that F varium may be one of the elusive pathogenic factors in UC.
ACCESSION #
9758412

 

Related Articles

  • In this issue.  // Nature Reviews Microbiology;Feb2010, Vol. 8 Issue 2, p83 

    The article discusses various reports published within the issue, including one by Lautaro Diacovich and Jean-Pierre Gorvel on mechanisms employed by pathogenic intracellular bacteria, one by Emmanuel Lemichez and colleagues on the mechanisms of pathogenic bacteria to promote adhesion and...

  • The relation between Helicobacter pylori and ulcerative colitis. CANER, Sedat; ALTINBAŞ, Akif; YEŞİL, Yusuf; BEYAZIT, Yavuz; YILMAZ, Baris; YÜKSEL, Osman // Turkish Journal of Medical Sciences;2014, Vol. 44 Issue 5, p820 

    Background/aim: Besides some genetic explanations of the native course of ulcerative colitis (UC), the most attributable factors are pathogenic bacterial agents. There are some conflicting data about the relationship between Helicobacter pylori and the rate of UC in the literature. Therefore, we...

  • Metronidazole and Ciprofloxacin for Pouchitis Treatment. Deresinski, Stan; Kwok-Yung Yuen // Clinical Infectious Diseases;3/15/2005, Vol. 40 Issue 6, p1 

    The article presents information on the use of metronidazole and ciprofloxacin for pouchitis treatment. Pouchitis is the major long-term complication after ileal pouch-anal anastomosis for ulcerative colitis. Metronidazole and ciprofloxacin are commonly used for treatment; however, nothing is...

  • Vertebral fractures in patients with inflammatory bowel disease COMPARED with a healthy population: a prospective case-control study. Angeles Vquez, Ma; Lopez, Enrique; Jos Montoya, Ma Jos; Giner, Merc; Prez-Temprano, Ramon; Prez-Cano, Ramon // BMC Gastroenterology;2012, Vol. 12 Issue 1, p47 

    Background: A prospective study was performed to compare the prevalence of morphometric vertebral fractures (MVF) between patients with inflammatory bowel disease (IBD) and healthy subjects and to identify predictive factors of fracture. Methods: A total of 107 patients with IBD (53 with Crohn's...

  • Multiple copies of AMT2 are prerequisite for the apple pathotype of Alternaria alternata to produce enough AM-toxin for expressing pathogenicity. Harimoto, Yoshiaki; Tanaka, Takayoshi; Kodama, Motoichiro; Yamamoto, Mikihiro; Otani, Hiroshi; Tsuge, Takashi // Journal of General Plant Pathology;Jun2008, Vol. 74 Issue 3, p222 

    The apple pathotype of Alternaria alternata produces the cyclic depsipeptide AM-toxin and causes Alternaria blotch of apple. Previously, we cloned AMT2 from the apple pathotype as an orthologue of AFTS1, which is required for biosynthesis of the decatrienoic acid ester AF-toxin I of the...

  • Enterotoxigenic and Genetic Profiles of Bacillus cereus Strains of Food Origin in Brazil. ARAGON-ALEGRO, LINA CASALE; PALCICH, GABRIELA; LOPES, GRACIELA VOLZ; RIBEIRO, VINÍCIUS BUCCELLI; LANDGRAF, MARIZA; DESTRO, MARIA TERESA // Journal of Food Protection;Oct2008, Vol. 71 Issue 10, p2115 

    In Brazil, the incidence of Bacillus cereus outbreaks is unknown, and there is little information about B. cereus occurrence in food. In addition, data on toxin production and genetic characterization of the B. cereus isolates cannot be found. This pathogen causes two distinct types of...

  • Bacterial toxin effector-membrane targeting: outside in, then back again. Geissler, Brett // Frontiers in Cellular & Infection Microbiology;May2012, Vol. 2, p1 

    Pathogenic bacteria utilize multiple approaches to establish infection and mediate their toxicity to eukaryotic cells. Dedicated protein machines deposit toxic effectors directly inside the host, whereas secreted toxins must enter cells independently of other bacterial components. Regardless of...

  • Prepore for a breakthrough. Bayley, Hagan; Jayasinghe, Lakmal; Wallace, Mark // Nature Structural & Molecular Biology;May2005, Vol. 12 Issue 5, p385 

    Claims that the key to understanding bacterial pathogenicity is the mechanism by which water-soluble protein toxins assemble on cell membranes to form oligomeric bilayer-spanning pores. Reconstructions from cryo-electron micrographs of three-dimensional pore and prepore structures of the...

  • mADP-RTs: versatile virulence factors from bacterial pathogens of plants and mammals. Wirthmueller, Lennart; Banfield, Mark J. // Frontiers in Plant Science;Jun2012, Vol. 3, p1 

    Mono ADP-ribosyltransferases (mADP-RTs) are a family of enzymes that cleave NAD(+) and covalently attach the ADP-ribosyl moiety to target proteins. mADP-RTs are well established as important virulence factors of bacteria that infect mammals. Cholera toxin, pertussis toxin, and diphtheria toxin...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics