Anderson, N.E.; Hayes, P.; Therapondos, G.; Boyter, A.C.; Smith, K.D.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA105
Academic Journal
Aims and Hypothesis: The acute phase response to injury or infection results in both qualitative and quantitative alterations in the expression of plasma proteins of hepatic origin. Many of these biomolecules are glycosylated with oligosaccharide chains that become structurally modified in certain diseases. The acute phase glycoprotein, AGP undergoes increased production and altered glycosylation of its five oligosaccharide chains in several physiological and pathological conditions. We hypothesise that subtle alterations in AGP glycosylation could be diagnostic for distinguishing individual liver diseases (LD) specifically hepatitis and its progression from/to other conditions. Methods: The glycosylation of AGP from the plasma of patients with alcoholic liver disease (ALD), primary biliary cirrhosis (PBC), and viral hepatitis was determined by high pH anion exchange chromatography. Lectin (concanavalin A) affinity chromatography was used to analyse the branching pattern of the oligosaccharide chains. Fucosyltransferase (FT) and fucosidase (FD) levels were determined by ELISA. Results: The hepatitis samples were clearly distinguishable from the other LD in terms of branching (con A ratio) and fucosylation of AGP oligosaccharide chains together with the appearance of the unusual monosaccharide N-acetylgalactosamine (GalNAc) on the oligosaccharide chains. Additionally lower than normal levels of fucosidase were detected in the plasma of hepatitis C patients. Conclusions: Variations in AGP glycosylation can be used to detect and distinguish between specific LD particularly hepatitis C in terms of unique monosaccharide composition and branching of the oligosaccharide chains. This information could be utilised clinically to detect the progression of hepatitis C to cirrhosis.


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