TITLE

ROLE OF PRO-INFLAMMATORY CYTOKINES IN ENTEROCYTE MEDIATED ELIMINATION OF ENCEPHALITOZOON INTESTINALIS

AUTHOR(S)
Zaalouk, T.K.; McDonald, V.; Blanshard, C.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA100
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: E intestinalis, a microsporidian pathogen in man, has emerged as a serious opportunistic ,pathogen in HIV infected individuals. in this study the development of the parasite in different cell lines was investigated. In addition, the effect of pro-inflammatory cytokines on this development was analysed. Methods: Seventy percent confluent monolayers of human (HT-29, Caco-2) colonic and murine (CMT-93) rectal adenocarcinoma cell lines were incubated with 4×10[sup 5] E intestinalis spores at 37°C and the cells were examined for intracellulor parasitic development after 24, 48, and 72h. In cytokine experiment cells were cultured in the presence of the cytokine(s) for 24 h prior to inoculation of E. intestinalis, and for a further 72h after inoculation in a concentration that was previously found not to damage the cells. Results: Our results showed E intestinalis was able to establish considerable infection in CMT-93 but, surprisingly, not in the human cell lines. The different stages of intracellular parasitic development were clearly visible in the CMT-93 cell line, making it a suitable in vitro model to study the infection in intestinal epithelium. Our results showed even small concentrations of IFN-α inhibited the development of the parasite by more than 90%. TNF-α and IL-6 also significantly reduced the infection and a combination of both cytokines had significantly higher inhibitory effect than either cytokine alone, suggesting that they have a synergistic effect. Conclusions: Observations from this study suggest that the CMT-93 cell line is appropriate for use in an in vitro model of E intestinalis infection. The requirement for IFN-α indicates that a Th1 response is important in control of infection. Additionally, enterocyte derived cytokines TNF-α and IL-6 may have an important role in intestinal immunity to infection.
ACCESSION #
9747967

 

Related Articles

  • Is the high virulence of HIV-1 an unfortunate coincidence of primate lentiviral evolution? Kirchhoff, Frank // Nature Reviews Microbiology;Jun2009, Vol. 7 Issue 6, p467 

    In the subset of primate lentiviruses that contain a vpu gene — HIV-1 and its simian precursors — the Nef protein has lost the ability to down-modulate CD3, block T cell activation and suppress programmed death. Vpu counteracts a host restriction factor induced by the inflammatory...

  • Polymorphisms of the human IFNG gene noncoding regions. Bream, Jay H.; Carrington, Mary; O’Toole, Stefanie; Dean, Michael; Gerrard, Bernard; Hyoung Doo Shin; Kosack, Daniel; Modi, William; Young, Howard A.; Smith, Michael W. // Immunogenetics;Jan2000, Vol. 51 Issue 1, p50 

    Interferon gamma (IFN-gamma) is a multifunctional cytokine that is essential in the development of Th1 cells and in cellular responses to a variety of intracellular pathogens including human immunodeficiency virus (HIV-1). We screened genomic DNA samples from a predominately Caucasian male...

  • Immunrekonstitutionssyndrome. Meyer-Olson, D.; Ernst, D.; Stoll, M. // Zeitschrift für Rheumatologie;Apr2012, Vol. 71 Issue 3, p187 

    The immune reconstitution inflammatory syndrome (IRIS) represents a heterogeneous group of conditions. Whilst they typically present in HIV-infected patients with advanced immunodeficiency, IRIS have also been described in HIV-negative patients with immune reconstitution due to other causes of...

  • Role of human innate lymphoid cells in IMID. Spits, Hergen; Bernink, Jochem; Peters, Charlotte; Mjosberg, Jenny // Journal of Translational Medicine;2012, Vol. 10 Issue Suppl 3, p1 

    The article discusses the role of human innate lymphoid cells (ILC) in immune-mediated inflammatory diseases (IMID). It says that the ILC are immune cells that lack a specific antigen receptors, but possess the capacity to make an array of effector cytokines. It mentions that the ILC involves in...

  • Comparison of the Virulence Potential of Acinetobacter Strains from Clinical and Environmental Sources. Tayabali, Azam F.; Nguyen, Kathy C.; Shwed, Philip S.; Crosthwait, Jennifer; Coleman, Gordon; Seligy, Verner L. // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Several Acinetobacter strains have utility for biotechnology applications, yet some are opportunistic pathogens. We compared strains of seven Acinetobacter species (baumannii, Ab; calcoaceticus, Ac; guillouiae, Ag; haemolyticus, Ah; lwoffii, Al; junii, Aj; and venetianus, Av-RAG-1) for their...

  • Immunity Traits in Pigs: Substantial Genetic Variation and Limited Covariation. Flori, Laurence; Gao, Yu; Laloë, Denis; Lemonnier, Gaëtan; Leplat, Jean-Jacques; Teillaud, Angé lique; Cossalter, Anne-Marie; Laffitte, Joëlle; Pinton, Philippe; de Vaureix, Christiane; Bouffaud, Marcel; Mercat, Marie-José; Lefèvre, François; Oswald, Isabelle P.; Bidanel, Jean-Pierre; Rogel-Gaillard, Claire // PLoS ONE;2011, Vol. 6 Issue 7, p1 

    Background: Increasing robustness via improvement of resistance to pathogens is a major selection objective in livestock breeding. As resistance traits are difficult or impossible to measure directly, potential indirect criteria are measures of immune traits (ITs). Our underlying hypothesis is...

  • Caveats Regarding the Use of IL-10 and IL-10 Antagonist as Immunotherapeutic Factors. Colino, Jesus // Letters in Drug Design & Discovery;Oct2007, Vol. 4 Issue 7, p487 

    IL-10 is an anti-inflammatory cytokine widely regarded as an inhibitor of immunity. Thus IL-10 and IL-10 antagonist have been proposed as promising therapeutic factors for the treatment of inflammatory diseases and pathogen infections. Here the potential risk of favoring secondary pathogen...

  • Kinases conquer the inflammasomes. Neumann, Konstantin; Ruland, Jürgen // Nature Immunology;Dec2013, Vol. 14 Issue 12, p1207 

    No abstract available.

  • The Agent of Human Granulocytic Ehrlichiosis Induces the Production of Myelosuppressing... Klein, Marina B.; Shuxian Hu; Chao, Chun C.; Goodman, Jesse L. // Journal of Infectious Diseases;7/1/2000, Vol. 182 Issue 1, p200 

    Examines the pathogenic role of inflammatory mediators in human granulocytic ehrlichiosis (HGE) infection based on cytokine production by untreated and dimethyl sulfoxide-treated HL-60 and by normal bone marrow cells. Modified sandwich enzyme-linked immunosorbent assays; Effect of HGE infection...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics