TITLE

THE EFFECT OF PEANUT LECTIN ON INTESTINAL PROLIFERATION, ACF AND TUMOUR DEVELOPMENT IN COLONIC CARCINOGENESIS

AUTHOR(S)
Mandir, N.; FitzGerald, A.J.; Goodlad, R.A.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA98
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Peanuts lectin (PNA) is a highly specific carbohydrate binding protein found in the diet and PNA may be harmful, as it is mitogenic to the human colon. Aberrant crypt foci (ACFs) are early precursor lesions of the adenoma-carcinoma sequence. They are found in rAP and sporadic patients and maybe associated with the early stages of adenoma development. Aims: To investigate intestinal growth, crypt fission (a process by which crypts split Iongitually to produce two new crypts), ACF, and tumour occurrence in rats. Methods: Rats were given a small dose (40 µg/rat/day) of PNA for 28 weeks, with or without DMH (20 mg/kg weekly). Carnoy's fixed tissue was stained with the Feulgen reaction and carefully microdissected to reveal individual crypts. Crypt cell production (following vincristine induced metaphase arrest) and crypt fission were scored. The number and size of polyps and ACFs in the colon were scored. Results: PNA significantly increased crypt cell production in the distal colon (p = 0.02) with DMH having no effect on proliferation. DMH significantly increased crypt fission (p < 0.001), but PNA had no effect on fission. Tumour distribution revealed two sites of tumour development, poorly differentiated mucous adenocarcinoma (Gp 3) in the proximal colon, and well differentiated tubular adenocarcinoma (Gp 1) in the distal colon, but ACFs were mainly confined to the distal colon. ACF multiplicity varied an average of 2-4 crypts per focus. Conclusion: Peanut lectin can have trophic actions on the colon but no effects on tumour and ACrs number were seen. ArCs may only be predictive of distal colon cancer.
ACCESSION #
9747951

 

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