TITLE

COLONIC MICROFLORA METABOLITES ALTER THE PROTECTIVE CAPACITY OF THE COLONIC MUCUS LAYER IN VIVO

AUTHOR(S)
Brownlee, I.A.; Havler, M.E.; Dettmar, P.W.; Allen, A.; Pearson, J.P.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA95
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This study aimed to determine the effects of reactive oxygen species (ROS) and butyrate on the protective capacity of the colonic mucus layer in vivo. An intravital microscopy technique was used to measure the maximal thickness and replenishment rates (rate of mucus secretion after partial removal of the layer by suction) of the colonic mucus layer of anaesthetised male Wistar rats (n = 5 for each treatment) over 5 h under 7 mM butyrate (normal concentration in the rat colon), as well as ROS solutions containing 0.5 mM Fe[sup ++]/EDTA mixed with 5-50 mM H[sub 2]O[sub 2]. These effects were compared to saline controls (n = 5). Maximal mucus thickness was not affected by 7 mM butyrate or 10 mM H[sub 2]O[sub 2], but 5 mM H[sub 2]O[sub 2] significantly increased maximal mucus thickness, whereas ROS solutions containing more than 10 mM peroxide (ie 17.5, 25, and 50 mM) significantly reduced the thickness of the mucus barrier (50% in the case of 50 mM H[sub 2]O[sub 2]). 7 mM butyrate significantly increased mucus layer replenishment rate over the first hour of measurement (×3 faster than the saline control. Measurement of the effect of ROS on colonic mucus layer replenishment was hampered by release of plasma exudate and red blood cells from the colonic mucosa. This suggests that Iow millimolar concentrations of butyrate and ROS may increase the protective capacity of the colonic mucus layer, possibly as a cytoprotective response. Higher levels of ROS appear to be damaging to both the mucus layer and mucosa, especially once the mucus layer is depleted. This study shows that bacterial metabolites may alter the protective potential of the colonic mucus barrier.
ACCESSION #
9747926

 

Related Articles

  • Sickle Cell Trait Detected on Colonic Biopsy. Li-cheng Wu, Mark // International Journal of Surgical Pathology;Apr2008, Vol. 16 Issue 2, p185 

    A photograph is presented depicting sickled erythrocyte in specimen from colonic biopsy of patient with sickle cell trait.

  • Oxygen Therapy for Pneumatosis Coli. Down, R.H.L.; Castleden, W.M. // British Medical Journal;3/1/1975, Vol. 1 Issue 5956, p493 

    Analyzes a case of patient treated with a high concentration of oxygen for pneumatosis coli in Great Britain. Clinical characteristics of the cysts in the submucosa of the colon; Technique to confirm the diagnosis of the disease; Issue regarding the aetiology of pneumatosis coli.

  • Reinforcing the mucus: a new therapeutic approach for ulcerative colitis? Gibson, P. R.; Muir, J.G. // Gut;Jul2005, Vol. 54 Issue 7, p900 

    Luminal delivery of phosphatidyl rich phospholipids appears to reduce mucosal inflammatory activity in a high proportion of patients with chronically active ulcerative colitis. The simplicity and apparent safety of this therapeutic approach offers new insights into the importance of the mucus...

  • Recent Studies on the Physiology of the Human Intestine: Their Application to Clinical Problems∗. Bargen, J. Arnold; Dixon, Claude F. // American Journal of Gastroenterology;Oct1982, Vol. 77 Issue 10, p708 

    Presents several medical studies concerning the application of the physiology of the human intestine to clinical problems. Functions of the colon; Factors that can affect the secretion of mucus in the large intestine; Explanations behind the secretion of mucus; Effects of the drugs used to...

  • Carbogen Breathing Differentially Enhances Blood Plasma Volume and 5-Fluorouracil Uptake in Two Murine Colon Tumor Models with a Distinct Vascular Structure. Van Laarhoven, Hanneke W. M.; Gambarota, Giulio; Lok, Jasper; Lammens, Martin; Kamm, Yvonne L. M.; Wagener, Theo; Punt, Cornelis J. A.; Van der Kogel, Albert J.; Heerschap, Arend // Neoplasia;Jun2006, Vol. 8 Issue 6, p477 

    For the systemic treatment of colorectal cancer, 5-fluorouracil (FU)--based chemotherapy is the standard. However, only a subset of patients responds to chemotherapy. Breathing of carbogen (95% O2 and 5% CO2) may increase the uptake of FU through changes in tumor physiology. This study aims to...

  • Evaluation of the effects of hyperbaric oxygen treatment and enoxaparin on left colon anastomosis. An experimental study. KEMIK, O.; ADAS, G.; ARIKAN, S.; GURLULER, E.; DOGAN, Y.; TOKLU, A. S.; KAPRAN, Y.; KUNTSAL, L.; PURISA, S.; KEMIK, A. // European Review for Medical & Pharmacological Sciences;Sep2013, Vol. 17 Issue 17, p2286 

    BACKGROUND: Surgical interventions on left colon lead to high morbidity. The problems in wound healing are the main cause of this morbidity. Hypoxia retards wound healing and hyperbaric oxygen treatment (HBOT) has an anti-hypoxic effect. MATERIALS AND METHODS: In this experimental study we...

  • Indications for hyperbaric oxygen therapy.  // DVM: The Newsmagazine of Veterinary Medicine;Apr2012, Vol. 43 Issue 4, p35 

    The article offers information on the concept of hyperbaric oxygen therapy. It mentions that the therapy allows patient to breathe high concentrations of oxygen. It mentions that hyperbaric treatment is used in the treatment of Lyme disease, colon torsions and peripheral neuropathies. It states...

  • Faecal urobilinogen levels and pH of stools in population groups with different incidence of cancer of the colon, and their possible role in its aetiology. Malhotra, S. L. // Journal of the Royal Society of Medicine;Sep1982, Vol. 75 Issue 9, p709 

    Mean faecal urobilinogen levels and the pH of stools were both found to be higher in subjects from a population group at high risk of developing cancer of the colon than in subjects matched for age, sex and socioeconomic status from a low-risk population group. An alkaline reaction of the colon...

  • Current Concepts of the Etiopathogenesis of Inflammatory Bowel Disease. Jayanthi, V.; Probert, C. S. J.; Sher, K. S.; Mayberry, J. F. // American Journal of Gastroenterology;Nov1991, Vol. 86 Issue 11, p1566 

    Focuses on the theories and hypotheses about the etiology of inflammatory bowel disease. Recurrence of disease close to anastomoses and remission after stricturoplasty; Role of human colonic mucus in nonimmunological defense system; Expression of any susceptible gene in the gastrointestinal tract.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics