TITLE

Optimization of matrix solid-phase dispersion extraction for the chromatographic analysis of flavones in cranberries

AUTHOR(S)
Linkevičiūtė, Aušra; Butkienė, Rita; Naujalis, Evaldas
PUB. DATE
July 2013
SOURCE
Chemija;2013, Vol. 24 Issue 3, p217
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A matrix solid-phase dispersion (MSPD) extraction method was applied for three flavones in cranberry (Vaccinium macrocarpon Ait.) matrix for the first time. The optimized MSPD procedure required a small amount of the sample (0.25 g), C18 bonded silica gel (1.0 g) as a dispersant solid-phase sorbent and small volumes of methanol using its aqueous solutions (10 mL) as eluting solvents. Identification and quantification of myricetin, quercetin and kaempferol were performed by HPLC-DAD-MS. The limit of detection varies from 0.04 µg/g to 0.08 µg/g with linear regression coefficients (r² > 0.999) for each compound. The recoveries of the analytes ranging from 59% to 111% with relative standard deviations less than 9.5% were checked using spiked freezedried cranberry samples. The MSPD method is simple, rapid and efficient for the extraction of flavones from cranberry samples.
ACCESSION #
97478347

 

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