TITLE

INFECTION OF MONGOLIAN GERBILS WITH CHINESE HELICOBACTER PYLORI STRAINS WITH FUNCTIONAL CAG PATHOGENICITY ISLANDS

AUTHOR(S)
Wang, J.; Jeremy, A.H.T.; Aboshkiwa, M.A.; Robinson, P.A.; Crabtree, J.E.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA80
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: To date only a few H pylori strains have been demonstrated to colonise Mongolian gerbils. The aims of this study were to establish stable colonisation of strains of H pylori from China in Mom golian gerbils and to assess the function of the cag pathogenicity island of infecting strains. Methods: Fresh clinical H pylori isolates from Chinese patients were inoculated into gerbils. At 4 to 6 weeks post-inoculation, infection status was evaluated by microbial culture, biopsy urease test and pathology. Sequencing of glmM and random amplified polymorphic DNA (RAPD) fingerprinting of DNA from cultured H pylori were used to evaluate the genetic identity of pre-inoculated and post-inoculated strains. The ability of pre- and post-inoculated strains to stimulate IL-8 transcription in L5F11 gastric epithelial cells was analysed using an IL-8 luciferase reporter assay. Results: Three of five inoculated clinical isolates colonised the Mongolian gerbils and induced chronic antral gastritis by 4 weeks post-infection. Each of the three pre- and post-inoculation cagA+ strains had identical glmM sequences and RAPD profiles, and stimulated luciferase secretion from L5F1 1 epithelial cells. The strain, which caused severe pathological changes, was selected for repeat infection to prove reproducible and stable colonisation. The cagA+, vacA s1c/m2a Chinese strain 42GX gave stable colonisation in the Mongolian gerbil and induced severe gastritis. Conclusions: This study demonstrates that Iow passage H pylori clinical isolates will successfully colonise Mongolian gerbils and the ability of the strains to stimulate IL-8 transcription in human gastric epithelial cells is maintained following infection in the Mongolian gerbil.
ACCESSION #
9747818

 

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