Raeiszadeh, M.; Fernando, N.; Holton, J.; Vaira, D.; Siavoshi, F.; Hosseini, A.; Kelly, P.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA79
Academic Journal
Different genotypes of H pylori have been observed in different locations of the world and identification of these genotypes may be important for understanding the clinical outcome of infection, the efficacy of antibiotic treatment, laboratory diagnosis, and possibly human migration patterns. Data on the vacA diversity from Iran and Africa are sparse. We compared the vacuolating cytotoxin alleles (vacA) and the cytotoxin associated gene A (cagA) in H pylori isolates from Iran and Zambia by means of PCR. A total of 31 H pylori isolates were studied from Iranian dyspeptic and 23 from Zambian dyspeptic patients. There was an equal distribution of s1 and s2 alleles in Iran, where slc was the predominant s1 subtype (10 of 16 s1 strains) with the m2 region predominating (26 out of 26 strains, with 5 stains unable to be typed in the "m" region). Isolates from Zambia mostly carried the sl allele (21 out of 23) with the s1 b allele being the most frequent (18 out of 21) and in the m region the m1 allele predominating (17 out of 21) with 2 stains non-typable for the m region. Using primer pairs for cagA 3' region, the percentages of cagA positive strains in Iran and Zambia were 41% (13 out of 31) and 86% (20 out of 23), respectively. Most of the cagA positive strains (85%) from Iran were of the non-Asian type and only two isolates were of the pure East Asian type. All of the 20 cagA positive strains from Zambia were of the non-Asian type and had the s1 allele. In Iran the cagA gene was found at a higher frequency in the s1 allele type (11 out of 16) as compared to the s2 strains (2 out of 15). Overall there was a strong association between the cagA marker and the s1 allele (p < 0.001).


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