Azooz, O.G.; De Silva, A.; Rampton, D.S.; Ballinger, A.B.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA61
Academic Journal
Introduction: Oxidative stress is a potential mechanism in the pathogenesis of inflammatory bowel disease (IBD) where reactive oxygen species (ROS) ore produced in excess by the intestinal mucosa. N-acetylcysteine (NAC) is a synthetic thiol compound which has antioxidant properties and suppresses production of NK-κB, and proinflammatory cytokines including TNF-α. Aims: The aim of this study was to assess the effect of NAC on the severity of colonic inflammation in a rat model of colitis. Methods: Colitis was induced in Wistar rats by intrarectal administration of 2,4,6 trinitrobenzenesulphonic acid (TNBS) and rats treated with i.p. NAC (50 or 200 mg/kg/day) or saline control (8/group). 16 healthy controls were treated with either NAC (50 mg/kg/day) or saline. Animals were sacrificed 5 days after induction of colitis. Severity of intestinal inflammation was assessed macroscopically, and by measurement of colonic tissue myeloperoxidase (MPO) and water content. ROS were measured by chemiluminescence (CLS). Results: TNBS induced distal colitis with ulceration, increased tissue MPO concentration and water content, and CLS (table). NAC reduced all these measures of intestinal inflammation except water content. There was no significant difference between 200 and 50 mg/kg of NAC. Conclusion: NAC has anti-inflammatory effects in the acute phase of TNBS-induced colitis. This treatment may be worth further assessment in the treatment of human IBD.


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