Orchard, T.R.; Ahmad, T.; Jewell, D.P.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA59
Academic Journal
Introduction: There has been much interest in the role of the innate immune system in inflammatory rheumatological conditions. CARD15 plays an important role in triggering an immune response to bacterial LPS, and polymorphisms in this gene are associated with Crohn's disease (CD). We have previously shown a genetic predisposition to ElMs, and have demonstrated abnormalities in the humoral immune response to enterobacteria in patients with arthritis associated with IBD. This study was performed to investigate whether polymorphisms in the CARD15 gene are associated with the presence of ElMs in IBD. Methods: DNA was obtained from 104 patients who had suffered Type 1 or Type 2 peripheral arthritis, erythema nodosum (EN) or uveitis (60 Crohn's disease and 44 ulcerative colitis). Genotyping for the 3 common po ymorphisms of CARD15 (Arg702Trp, Gly908Arg, and Leu1007fsinsC) was undertaken by SSP PCR, and allele frequencies were compared with 354 healthy controls and 244 CD patients. Results: Allele frequencies (%) are shown in the table below: ElMs were significantly associated with CARD15 polymorphisms, when compared to controls, particularly type 1 arthritis and EN. Further analysis was undertaken by IBD diagnosis, and the association between CARD15 polymorphisms and ElMs was seen in both UC and Crohn's patients. Discussion: CARD15 polymorphisms may be important in determining the ElMs of IBD, suggesting that bacteria may trigger them. Further studies are required to confirm these associations, and to investigate the interaction with previously described HLA associations.


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