Hart, A.L.; Kamm, M.A.; Knight, S.C.; Stagg, A.J.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA56
Academic Journal
Introduction: Dendritic cells (DC) are antigen-presenting cells present throughout the gastrointestinal tract that interact with bacteria and determine the subsequent T cell response. Probiotic bacteria are effective in the treatment of some inflammatory bowel diseases. Probiotic bacteria modulate immune function, but the effect of these bacteria on DC is unclear. This study aimed to determine whether probiotic bacteria influence DC phenotype and cytokine production. Methods: DC were identified by multi-colour flow cytometry as an HLA-DR+ lineage- (CD3-, CD14-, CD16-, CD19-, CD34-, CD56-) population in blood. Cell wall fractions of the 8 probiotic bacteria present in the probiotic combination VSL#3 in addition to Streptococcus faecium, Escherichia coli Nissle strain and lipopolysaccharide (LPS) were prepared by sonication and centrifugation and cultured with whole blood. Changes in maturation and co-stimulatory markers (CD80, CD86, CD83 and CD40) and cytokine production (IL-12 and IL-10) were analysed by flow cytometry. Results: The combination of organisms in VSL#3 downregulated IL-12 and upregulated IL-10 production by DC in a dose-dependent manner. All the individual components downregulated IL-12 production, but only the bifidobacteria strains enhanced IL-10 production. All the bacteria upregulated all the co-stimulatory and maturation markers with the exception of the bifidobacteria strains which downregulated CD80 expression. An anti-lL-10 antibody did not prevent this effect. LPS had opposing effects to VSL#3, enhancing IL-12 and decreasing IL-10 production. Co-culturing VSL#3 with LPS indicated crossregulation of the DC cytokine response. The enhanced IL-12 seen with LPS alone was reduced in the presence of VSL#3 but IL-10 production seen with VSL#3 alone was maintained in the presence of LPS. Conclusions: Probiotic bacteria differentially activate DC in vitro in a manner that may favour an anti-inflammatory T cell response.


Related Articles

  • Role of Dendritic Cell-Derived Cytokines in Immune Regulation. Yonghong Wan; Bramson, Jonathan // Current Pharmaceutical Design;Jul2001, Vol. 7 Issue 11, p977 

    Over the last several years, it has become increasingly clear that dendritic cells (DC) are not only critical for the initiation of T cell immunity, but these cells also determine the course of the subsequent immune response (i.e. tolerance vs. immunity, Th1 vs Th2). However, the mechanisms by...

  • From the editors.  // Nature Reviews Immunology;Sep2011, Vol. 11 Issue 9, p567 

    An introduction is presented in which the editor discusses various reports within the issue on topics including dendritic cell (DC) deficiency, immune responses, and the anti-inflammatory effects of exercise.

  • Immature, but not inactive: the tolerogenic function of immature dendritic cells. Mahnke, Karsten; Schmitt, Edgar; Bonifaz, Laura; Enk, Alexander H; Jonuleit, Helmut // Immunology & Cell Biology;Oct2002, Vol. 80 Issue 5, p477 

    Summary The induction of antigen-specific T cell tolerance and its maintenance in the periphery is critical for the prevention of autoimmunity. Recent evidence shows that dendritic cells (DC) not only initiate T cell responses, but are also involved in silencing of T cell immune responses. The...

  • NEW EMBO MEMBER'S REVIEW Dendritic cell regulation of immune responses: a new role for interleukin 2 at the intersection of innate and adaptive immunity. P. Ricciardi-Castagnoli; F. Granucci; I. Zanoni; S. Feau // EMBO Journal;6/1/2003, Vol. 22 Issue 11, p2546 

    Dendritic cells are professional antigen-presenting cells able to initiate innate and adaptive immune responses against invading pathogens. In response to external stimuli dendritic cells undergo a complete genetic reprogramming that allows them to become, soon after activation, natural killer...

  • Death, destruction, danger and dendritic cells. Austyn, Jonathan M. // Nature Medicine;Nov99, Vol. 5 Issue 11, p1232 

    Dendritic cells are known to be involved in recognition of foreign antigens and initiation of specific T-cell responses. The 'danger hypothesis' suggests that the immune system can also respond to endogenous signals of distress. New data indicate that dendritic cells are the first to respond to...

  • Kinetics of dendritic cell activation: impact on priming of TH1, TH2 and nonpolarized T cells. Langenkamp, Anja; Messi, Mara; Lanzavecchia, Antonio; Sallusto, Federica // Nature Immunology;Oct2000, Vol. 1 Issue 4, p311 

    To prime immune responses, dendritic cells (DCs) need to be activated to acquire T cell stimulatory capacity. Although some stimuli trigger interleukin 12 (IL-12) production that leads to T helper cell type 1 (TH1) polarization, others fail to do so and favor TH2 polarization. We show that after...

  • Dendritic cells and immune regulation in the liver. Lau, A.H.; Thomson, A.W. // Gut;Feb2003, Vol. 52 Issue 2, p307 

    Hepatic dendritic cells (DC) unquestionably play important roles in the induction and regulation of immune responses. Due to their paucity, functional characterisation of these important antigen presenting cells has been slow but use of DC growth factors (in particular GM-CSF and FIt3L) that...

  • Interaction of dendritic cells with mycobacteria: Where the action starts. Britton, Wj; Demangel, Caroline; Britton, Warwick J // Immunology & Cell Biology;Aug2000, Vol. 78 Issue 4, p318 

    Summary Dendritic cells (DC) are the major antigen-presenting cells in the induction of cellular responses to intracellular pathogens, such as mycobacteria. Recent studies have shown that they also play a critical role in the regulation of immune responses. The interaction of DC with microbial...

  • HLA-DR+ Immature Cells Exhibit Reduced Antigen-Presenting Cell Function But Respond to CD40 Stimulation. Pinzon-Charry, Alberto; Maxwell, Tammy; Prato, Sandro; Furnival, Colin; Schmidt, Chris; López, José Alejandro // Neoplasia;Dec2005, Vol. 7 Issue 12, p1123 

    Dendritic cells (DC) have been implicated in the defective function of the immune system during cancer progression. We have demonstrated that patients with cancer have fewer myeloid (CD11c+) and plasmacytoid (CD123hi) DC and a concurrent accumulation of CD11c-CD123- immature cells expressing...


Read the Article


Sign out of this library

Other Topics