Hart, A.L.; Kamm, M.A.; Knight, S.C.; Stagg, A.J.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA56
Academic Journal
Introduction: Dendritic cells (DC) are antigen-presenting cells present throughout the gastrointestinal tract that interact with bacteria and determine the subsequent T cell response. Probiotic bacteria are effective in the treatment of some inflammatory bowel diseases. Probiotic bacteria modulate immune function, but the effect of these bacteria on DC is unclear. This study aimed to determine whether probiotic bacteria influence DC phenotype and cytokine production. Methods: DC were identified by multi-colour flow cytometry as an HLA-DR+ lineage- (CD3-, CD14-, CD16-, CD19-, CD34-, CD56-) population in blood. Cell wall fractions of the 8 probiotic bacteria present in the probiotic combination VSL#3 in addition to Streptococcus faecium, Escherichia coli Nissle strain and lipopolysaccharide (LPS) were prepared by sonication and centrifugation and cultured with whole blood. Changes in maturation and co-stimulatory markers (CD80, CD86, CD83 and CD40) and cytokine production (IL-12 and IL-10) were analysed by flow cytometry. Results: The combination of organisms in VSL#3 downregulated IL-12 and upregulated IL-10 production by DC in a dose-dependent manner. All the individual components downregulated IL-12 production, but only the bifidobacteria strains enhanced IL-10 production. All the bacteria upregulated all the co-stimulatory and maturation markers with the exception of the bifidobacteria strains which downregulated CD80 expression. An anti-lL-10 antibody did not prevent this effect. LPS had opposing effects to VSL#3, enhancing IL-12 and decreasing IL-10 production. Co-culturing VSL#3 with LPS indicated crossregulation of the DC cytokine response. The enhanced IL-12 seen with LPS alone was reduced in the presence of VSL#3 but IL-10 production seen with VSL#3 alone was maintained in the presence of LPS. Conclusions: Probiotic bacteria differentially activate DC in vitro in a manner that may favour an anti-inflammatory T cell response.


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