TITLE

IMMUNITY TO CRYPTOSPORIDIUM PARVUM: A ROLE FOR IL-4 IN THE EARLY ADAPTIVE IMMUNE RESPONSE

AUTHOR(S)
McDonald, S.A.C.; Bejaj-Elliott, M.; Smith, P.T.; Ballinger, A.B.; McDonald, V.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA55
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: Immunological control of Cryptosporidium parvum infection is dependent on a Th1 response with interferon-γ production. However, in a neonatal murine infection model we have shown that the Th2 cytokine IL-4 is also involved as BALB/c lL-4 knockout (KO) mice develop more intense oocyst shedding at the peak of infection (Day 7) than wild-type mice. Paradoxically, at this time, no increase in lb4 mRNA could be detected in the intestines of wild-type mice. The aim of this study was to characterise further IL-4 involvement in the early priming of immunity to C parvum. Methods: Neonatal BALB/c wild-type or SCID mice (which lack T and B cells) were infected at 4 or 7 days of age with a cervine isolate of C parvum and infections were subsequently measured microscopically from acid-fast stained smears of colonic contents. Murine recombinant IL-4 (1.0 and 0.75µg) was injected sc prior to infection and 6 h later. Rat anti-IL-4 IgG monoclonal antibody 11B11 (100µg) was injected prior to infection or 4 days post-infection. Results: Injection of BALB/c mice with IL-4 decreased the level of C parvum reproduction on day 7 by a factor of 4.6. Administration of anti-IL-4 to BALB/c mice prior to infection-but not 4 days post-infection—increased susceptibility to infection. To determine if an lL-4 dependent innate mechanism was at work, SCID mice were given anti-IL-4 prior to infection, but this did not increase parasite reproduction. Conclusion: IL-4 may act early in infection to boost the adaptive immune response to the intracellular mucosal parasite, C parvum.
ACCESSION #
9747654

 

Related Articles

  • Infectivity of a Cryptosporidium parvum Isolate of Cervine Origin for Healthy Adults and Interferon-g Knockout Mice. Okhuysen, Pablo C.; Rich, Stephen M.; Chappell, Cynthia L.; Grimes, Kevin A.; Widmer, Giovanni; Feng, Xiaochuan; Tzipori, Saul // Journal of Infectious Diseases;5/1/2002, Vol. 185 Issue 9, p1320 

    The infectivity of a Cryptosporidium parvum isolate of cervine origin (type 2, Moredun) propagated in calves was investigated simultaneously in healthy adult human volunteers and in interferon-γ knockout (GKO) mice. After exposure to 100-3000 oocysts, 16 volunteers recorded, for a duration of...

  • Interferon-gamma Is Required for Innate Immunity to Cryptosporidium parvum in Mice. Hayward, Anthony R.; Chmura, Katherine; Cosyns, Mary // Journal of Infectious Diseases;9/1/2000, Vol. 182 Issue 3, p1001 

    Determines whether the innate immunity of severe combined immunodeficiency (SCID) mice to Cryptosporidium parvum (CP) requires interferon (IFN)-gamma through breeding doubly immunodeficient C57BL/6 SCID-IFN-gamma knockout mice. Comparison of the outcome of CP infection in these animals with the...

  • Role of murine Peyer's patch lymphocytes against primary and challenge infections with Cryptosporidium parvum. Sang-Mee Guk; Yong-Yil Chai // Korean Journal of Parasitology;Sep2007, Vol. 45 Issue 3, p175 

    In order to determine the role of Peyer's patch lymphocytes (PPL) in self-clearing of Cryptosporidium parvum infection in murine models, changes in PPL subsets, their cytokine expression, and in vitro IgG1 and IgA secretions by PPL were observed in primary- and challenge-infected C57BL/6 mice....

  • A Protective Role for Interleukin 18 in Interferon γ–Mediated Innate Immunity to Cryptosporidium parvum That Is Independent of Natural Killer Cells. Choudhry, Naheed; Petry, Franz; van Rooijen, Nico; McDonald, Vincent // Journal of Infectious Diseases;Jul2012, Vol. 206 Issue 1, p117 

    Innate immunity against some intracellular parasitic protozoa involves interleukin 18 (IL-18)–mediated interferon γ (IFN-γ) production by natural killer (NK) cells, but the role of IL-18 in innate resistance to Cryptosporidium infection is unknown. Adult...

  • CCL20 Displays Antimicrobial Activity Against Cryptosporidium parvum, but Its Expression Is Reduced During Infection in the Intestine of Neonatal Mice. Guesdon, William; Auray, Gaël; Pezier, Tiffany; Bussière, Françoise I.; Drouet, Françoise; Le Vern, Yves; Marquis, Mathilde; Potiron, Laurent; Rabot, Sylvie; Bruneau, Aurelia; Werts, Catherine; Laurent, Fabrice; Lacroix-Lamandé, Sonia // Journal of Infectious Diseases;10/15/2015, Vol. 212 Issue 8, p1332 

    CCL20 is a chemokine with antimicrobial activity. We investigated its expression and role during neonatal cryptosporidiosis, a worldwide protozoan enteric disease leading to severe diarrhea. Surprisingly, during infection by Cryptosporidium parvum, CCL20 production by the intestine of neonatal...

  • MicroRNA-98 and let-7 Regulate Expression of Suppressor of Cytokine Signaling 4 in Biliary Epithelial Cells in Response to Cryptosporidium parvum Infection. Guoku Hu; Rui Zhou; Jun Liu; Ai-Yu Gong; Xian-Ming Chen // Journal of Infectious Diseases;7/1/2010, Vol. 202 Issue 1, p125 

    Expression of the cytokine-inducible Src homology 2 (CIS) protein and suppressors of cytokine signaling (SOCS) proteins represents an important element of host cell reactions in response to infection. We have demonstrated previously that Cryptosporidium parvum infection down-regulates...

  • Cryptosporidium parvum in Oysters from Commercial Harvesting Sites in the Ceasapeake Bay. Fayer, Ronald; Lewis, Earl J. // Emerging Infectious Diseases;Sep/Oct99, Vol. 5 Issue 5, p706 

    Oocysts of Cryptosporidium parvum, a zoonotic waterborne pathogen, can be removed by bivalve molluscs from contaminated water and retained on gills and in hemolymph. We identified oocysts of C. parvum in oysters from seven sites in the Chesapeake Bay area. These findings document the presence of...

  • Confronting Crypto. Stone, Leland Edward // Reeves Journal: Plumbing, Heating, Cooling;Oct96, Vol. 76 Issue 10, p18 

    Focuses on the issue of the presence of Cryptosporidium parvum in water. Mechanism of infection; Health aspects for humans; Government policy; Socioeconomic implications; Incidents of outbreaks; Water treatment solutions; Water testing.

  • Cryptosporidium parvum: Household Transmission. Current, William L. // Annals of Internal Medicine;3/15/94, Vol. 120 Issue 6, p518 

    Editorial. Presents observations on a research on the person-to-person transmission and household epidemiology of cryptosporidium parvum published in the March 1994 issue of 'Annals of Internal Medicine' journal. Rate of transmission in households; Result of the study; Views on the...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics