TITLE

FUNCTIONAL CANNABINOID RECEPTOR EXPRESSION IN NORMAL HUMAN COLONIC EPITHELIUM

AUTHOR(S)
Wright, K.L.; Rooney, N.; Tate, J.; Feeney, M.A.; Robertson, D.A.; Welham, M.J.; Ward, S.G.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA55
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and Aims: We investigated the presence of functional cannabinoid receptors in normal human colon and purified normal human colonic epithelial cells and human colonic epithelial cell lines, HT29 and CaCo. Methods: The location of both the CB1 and CB2 receptors in normal human colonic biopsies was determined by immunohistochemistry. Purified normal human colonic epithelial cells from resected tissue and human colonic epithelial cell lines were treated with both synthetic and endogenous cannabinoids in vitro. Phosphorylation of the p42/44 mitogen-activated protein kinases (MAPKs) and glycogen synthase kinase (GSK)-3α/β was tested by Western immunoblotting. Results: CB1 receptor expression was evident in colonic epithelium as well as smooth muscle and the submucosal myenteric plexus. The signal was particularly strong on the apical membrane of the epithelial cells. CB2 receptor expression was expressed on plasma cells and macrophages in the lamina propria. Both purified primary colonic epithelial cells and related cell lines responded to cannabinoids by activation of the p42/44 MAPK pathway and phosphorylation of GSK30alpha;/β. Conclusions: Given the accepted role of colonic epithelium in immune host responses and the inflammatory bowel diseases, the presence of functional cannabinoid receptors in normal human colon implies a role for the endocannabinoid system in normal gastrointestinal physiology, which may impact on mucosal immunity and modulate inflammation.
ACCESSION #
9747651

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics