TITLE

EXPRESSION OF THE CYTOPROTECTIVE TREFOIL PROTEINS TFF2 IN HUMAN GASTRIC AND DUODENAL BIOPSIS

AUTHOR(S)
Johns, C.E.; Newton, J.L.; Westley, B.R.; May, F.E.B.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA521
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: TFF2 is the second member of the trefoil family of peptides. These small proteins are found in epithelial surfaces and contribute to mucosal repair and protection. It has been suggested that the glycosylated form of TFF2 is more active inpromoting ulcer healing in rats. Immunohistochemical studies have demonstrated TFF2 in the antral glands of the stomach and the Brunner's glands of the duodenum. The relative amounts in these areas have not been quantified previously in man. Methodology: Seven patients attending for routine oesophagogastroduodenoscopy gave written informed consent. In each subject gastric biopsies were taken from within 2 cm of the pylorus and duodenal biopsies from each of the first, second and third parts of the duodenum. Separate parallel biopsies were taken for immunohistochemistry. The amount of TFF2 in the biopsies was measured by quantitative Western transfer analysis. Results: High glycosylated TFF2 levels were found in the gastric antrum and first part of the duodenum. There was no difference between these areas (means both 3.4 ng per µg total protein; p=0.99 ci -4.2,4.1). TFF2 was present in D2 in all subjects, and 4 had detectable TFF2 in D3 (mean 0.2 ng per µg protein). The proportion of total TFF2 that is non-glycosylated was greater in the duodenum than in the stomach, (means 7.3% and 3.1% respectively; p=0.03, ci -7.9 -0.44). The profile of TFF2 glycosylation in the duodenum was different from that in the stomach. Discussion: The presence of TFF2 in D3, beyond the pancreatic duct, where Brunner's glands are thought to be absent, indicates that Brunner's glands may not be the only source of TFF2 in the human duodenum, and that TFF2 is active in D3. The higher proportion of non-glycosylated TFF2 in the duodenum suggests this peptide may have a different function here.
ACCESSION #
9747621

 

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