TITLE

PROSTAGLANDIN E2 RECEPTOR (EP RECEPTOR) EXPRESSION IN PRE-MALIGNANT AND MALIGNANT GASTRIC MUCOSA

AUTHOR(S)
Wood, A.; Ballinger, A.B.; Smith, G.V.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA51
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Prostaglandin E[sub 2] (PGE[sub 2]) is significantly over-expressed in gastric adenocarcinoma and as a result of H pylori (Hp) infection. This is associated with over expression of cyclooxygenase 2. The effects of PGE[sub 2] are mediated by both nuclear and ce l surface receptors. The PGE[sub 2] cell surface (EP) receptors activate several intracellular homeostatic mechanisms and have been implicated in cell cycling. The expression of these receptors in gastric carcinogenesis is poorly understood. Methods: Paraffin embedded sections of normal gastric mucosa, Hp gastritis, mucosal atrophy, intestinal metaplasia (IM), dyspiasia and cancer were analysed immunohistochemically for the expression of the four subtypes of EP receptor. Polyclonal antibodies raised against these receptors were utilised. Controls comprised oesophageal and duodenal biopsies. Negative controls were analysed by substitution of the primary antibody with diluent or its pre-incubation with a specific control peptide for each receptor. Two observers scored each slide. Median scores were compared with a Mann-Whitney analysis. Discussion: Significant variations in EP receptor expression are seen in gastric pathology. EP4 receptors appear to be up-regulated in inflammatory conditions associated with Hp infection, whereas EP2 and EP3 are over-expressed in premaiignant and malignant tissue. These patterns of expression at the time of increased PGF2 synthesis, may provide a mechanism for increased ceil cycling and proliferation and play a significant role in inflammation and neoplasia.
ACCESSION #
9747612

 

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