Crnogorac-Jurcevic, T.; Lemoine, N.R.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA36
Academic Journal
In order to expand our understanding of the molecular changes underlying the complex pathology of pancreatic malignancy, global gene expression profiling of pancreatic adenocarcinoma compared to normal pancreatic tissue was performed. Human cDNA arrays comprising 9932 elements were interrogated with fluorescently labelled normal and adenocarcinoma samples using a reference control design, so that a pool of normal pancreatic tissues was always labelled with direct incorporation of Cy3 dCTP, while test samples (nine tumours, three normal pancreata and three cell lines) were always labelled with Cy5 dCTP. The enrichment with tumour cells was performed by manual trimming of the frozen blocks controlled by frequent microscopical examination of H&E stained sections. This resulted in enrichment of the specimens to at least 80% of tumour cells. The data were analysed for differential gene expression, which was confirmed by serial analysis of gene expression (SAGE), digital differential display (DDD) analysis and immunohistochemistry for selected cases. The array data were filtered to produce a total of 75 genes significantly upregulated or downregulated in pancreatic adenocarcinoma. Two of those showing the highest differential were members of the S100 family of Ca binding proteins (S100P and S100A6) and were selected for additional studies by immunohistochemistry. As neither of them is expressed in normal pancreatic tissues, they could represent potential markers for pancreatic carcinogenesis.


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