TITLE

HELICOBACTER PYLORI INFECTION IS ASSOCIATED WITH UPREGULATION OF MATRILYSIN (MMP-7) IN GASTRIC EPITHELIAL CELLS IN VIVO

AUTHOR(S)
Bebb, J.R.; Aviles, F.; Hand, N.; Zaitoun, A.; Atherton, J.C.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA18
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: MMP-7 (matrilysin) is a member of the metalloproteinase family of enzymes, which are important in normal and pathological remodelling of epithelial matrix interactions, it is upreguJated in gastric cancer. We have previously shown that H pylori co-culture induces upregulation of MMP-7 in epithelial cells in vitro at both the protein and RNA level, and that this is more marked for strains with an intact cag pathogenicity island. We now examined whether this occurs in viva. Methods: Gastric biopsies were taken at endoscopy from H pylori infected (n = 17) and uninfected (n = 16) patients, and MMP-7 expression examined by immunohistochemistry (paraffin-embedded sections), ELISA (R&D systems), and Real Time PCR. H pylori strains were cultured and PCR-typed for cagA, cagE, and vacA. For immunohistochemistry, slides were examined by two blinded observers and staining intensity was graded 0-4. Results were compared by Mann Whitney U test (immunohist) and t test (ELISA, Real Time PCR). Results: Epithelial ceils from H pylori infected patients stained more intensely for MMP-7 than uninfected patients both in antrum and corpus. This was most marked superficially in the antrum (median Hp+ 2, Hp- 1, p < 0.05) and in the proliferative zone in the corpus (median Hp+ 3, Hp- 2, p < 0.05). There was also a significant increase in MMP-7 staining inflammatory cells in infected patients. ELISA confirmed these results with a threefold increase in infected patients (Hp+ 0.182 v Hp- 0.059, p = 0.009). Real Time PCR demonstrated upregulation of MMP-7 at RNA level (antrum Hp+ 0.038 v Hp0.008, p = 0.007). It was not possible to correlate changes with strain type due to small numbers of cag-negative strains. Conclusion: This study demonstrates increased expression of MMP-7 in vivo in nonmalignant gastric epithelial cells colonised by H pylori. We speculate that upregulation of MMP-7 in H pylori gastritis may play a role in H pylori induced gastric carcinogenesis.
ACCESSION #
9747377

 

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