Taha, A.S.; Faccenda, E.; Angerson, W.J.; Kelly, R.W.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA18
Academic Journal
Background and Aims: Recent in vitro and animal model studies have identified a number of epithelial peptides with antimicrobial and potentially gastroprotective activities. To investigate their relevance to human peptic ulcer disease, we assessed gastric epithelial secretory leukocyte proteinase inhibitor (SLPI) and human beta defensins, HBD1, HBD2, and HBD5 in a range of peptic disorders. Methods: Gastric biopsies were taken from 52 patients, median age of 55 years, including 31 males and 8 smokers. Expression of SLPI, HBD1, HBD2, and HBD5 mRNA was determined using real time quantitative PCR. Histological assessment was carried out on biopsies taken from the same anatomical regions. The activity of gastritis was graded on a 0-3 scale, 0 being normal and 3 severe. Specimens carried code numbers for blind assessment. Results: The gastric antrum had o median SLPI level of 0.93 and HBD1 of 0.42, compared with 0.13 (p = 0.001) and 0.08 (p = 0.002), respectively, in the gastric body. The antral histological scores correlated positively with HBD2 expression (r = 0.69; p < 0.001) and negatively with HBD1 (r = -0.47; p = 0.006), particularly in the absence of aspirin. Patients with H pylori gastritis, gastric, or duodenal ulcers had lower expression of HBD1 and greater expression of HBD2 and this pattern was only partially reversed by H pylori eradication. The intake of Iow dose aspirin, 75 mg daily, by 16 patients had no influence on epithelial peptides of those free of H pylori infection. However, in the infected group aspirin was associated with lower expression of SLPI (0.33 v 0.58; p = 0.029) and higher expression of HBD5 (42.3 v 3.25; p = 0.039). Conclusions: The expression of some endogenous epithelial antimicrobial peptides is influenced by the anatomical site, the grade of gastritis, H pylori, and peptic ulceration. It can also be modulated by Iow dose aspirin particularly in subjects infected with H pylori.


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