TITLE

ELEVATED PLASMA CONCENTRATIONS OF AMIDATED GASTRIN CAUSE INCREASED CRYPT SURVIVAL IN MURINE INTESTINAL EPITHELIA AFTER γ RADIATION

AUTHOR(S)
Ottewell, P.D.; Watson, A.J.M.; Wang, T.C.; Varro, A.; Dockray, G.J.; Pritchard, D.M.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA8
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background and Aims: Amidated gastrin has well characterised mitogenic and morphogenic properties in the stomach, but its role in the distal intestine remains unclear. We have previously demonstrated no differences in the levels of apoptosis and mitosis in small intestinal and colonic epithelia of mice that overexpress amidated gastrin (INSGAS) compared to their wild-type counterparts, either in the untreated state or 4.5 hours following 8 Gy γ radiation. In order to complete our analysis of the effects of hypergastrinaemia upon the distal intestine in vivo, we have now investigated the effects of elevated plasma concentrations of amidated gastrin upon intestinal crypt survival following γ radiation. Methods: Mice analysed were adult INS-GAS and their wild-type counterparts (FVB/N). CIonogenic crypt survival was assessed by light microscopy of small intestinal and colonic crypts four days after 10, 12, or 14Gy γ radiation. We confirmed that the differences observed were specifically induced by hypergastrinaemia by analysing INS-GAS mice treated with the specific gastrin/CCKB receptor antagonist YF476 and FVB/N mice treated with omeprazole. Results: Four days following 12 and 14Gy γ radiation, INS-GAS mice exhibited significantly higher (∼3 fold) small intestinal and colonic crypt survival compared with their wild-type counterparts. INSGAS mice treated with YF476 showed significantly lower (∼4 fold) small intestinal and colonic crypt survival after 14Gy γ radiation compared with mice receiving vehicle alone. FVB/N mice dosed with omeprazole to induce hypergastrinaemia showed significantly increased (∼4 fold) survival of small intestinal and colonic crypts after 14Gy γ radiation. Conclusions: (a) Increased small intestinal and colonic crypts survival is observed in mice with elevated plasma concentrations of amidated gastrin following γ radiation. (b) This protective effect of hypergastrinaemia occurs as a...
ACCESSION #
9747301

 

Related Articles

  • Serum pepsinogen and gastrin do not predict intestinal metaplasia in Caucasian subjects. Gatta, L.; Vaira, D.; Rugge, M.; Ricci, C.; Russo, V.; Leandro, G.; Menegatti, M.; Perna, F.; Tampieri, A.; Miglioli, M.; Vakil, N. // Gut;Sep2002 Supplement 2, Vol. 51, pA44 

    Aim: The aim of this study was to determine the predictive value of serum pepsinogen and gastrin in predicting the presence of intestinal metaplasia in subjects with Helicobacter pylori (HP) infection and to determine the effect of eradication on these serum markers. Methods: 169 donors infected...

  • © CHOLECYSTOKININ-LIKE PEPTIDES IN BRAIN AND INTESTINE OF OBESE-HYPERGLYCAEMIC MICE. Hansky, Jack; Ho, Patricia // Australian Journal of Experimental Biology & Medical Science;Dec1979, Vol. 57 Issue 6, p575 

    Examines the cholecystokinin (CCK)-like peptides in brain and intestine of obese-hyperglycaemic mice. Measurement of CCK-like peptides by radioimmunoassay employing an antiserum to CCK octapeptide and gastrin; Concomitant assay of tissue extracts with a gastrin specific antibody; Level of CCK in...

  • Small intestine.  // Mayo Clinic Health Letter;Nov2007, Vol. 25 Issue 11, p7 

    The article provides facts related to the small intestine, also known as the small bowel. It describes the three sections in the small intestine, which include the duodenum, the jejunum and ileum. It discusses the digestive process in the small intestine. It explains the role of various...

  • Use of interfering RNA to investigate the role of endogenous gastrin in the survival of gastrointestinal cancer cells. Grabowska, A. M.; Hughes, J.; Watson, S. A. // British Journal of Cancer;2/12/2007, Vol. 96 Issue 3, p464 

    Gastrin isoforms, acting through a variety of receptors, have proliferative and anti-apoptotic effects on gastrointestinal (GI) cancers. A small interfering RNA (siRNA) targeting the gastrin gene was used to investigate the role of endogenous gastrin in GI cancer cell survival. Downregulation of...

  • Relationship between expression of gastrin, somatostatin, Fas/FasL and caspases in large intestinal carcinoma. Jia-Ding Mao; Pei Wu; Ying-Lin Yang; Jian Wu; He Huang; Lambert, Rene; Yamamoto, Takayuki // World Journal of Gastroenterology;5/14/2008, Vol. 14 Issue 18, p2802 

    AIM: To explore the correlation between the mRNAs and protein expression of gastrin (GAS), somatostatin (SS) and apoptosis index (AI), apoptosis regulation gene Fas/FasL and caspases in large intestinal carcinoma (LIC). METHODS: Expression of GAS and SS mRNAs were detected by nested RT-PCR in 79...

  • Reduced Mitotic Activity of Intestinal Mucosal Cells in Germ-free Rats Mono-associated with Toxin-Producing Clostridium difficile. Banasaz, M.; Åkerlund, T.; Norin, E.; Burman, L.; Midtvedt, T. // Microbial Ecology in Health & Disease;Dec2003, Vol. 15 Issue 4, p146 

    The aim of the study was to investigate whether, and to what extent, mono-association of germ-free rats with Clostridium difficile influences intestinal cell kinetics and epithelial structure. Young male germ-free rats were mono-associated with either a toxin-producing (group I) or a...

  • Radioprotection to small intestine of the mice against ionizing radiation by semiquinone glucoside derivative (SQGD) isolated from Bacillus sp. INM-1. Patel, Dev; Bansal, Deen; Mishra, Saurabh; Arora, Rajesh; Sharma, Ashok; Jain, Swatantra; Kumar, Raj // Molecular & Cellular Biochemistry;Nov2012, Vol. 370 Issue 1/2, p115 

    Ionizing irradiation induces severe damage to the intestinal crypt cells which are responsible for renovation and maintenance of the intestinal cellular architecture. Therefore, protection of intestinal cells and tissue against lethal irradiation using a semiquinone glucoside derivative (SQGD)...

  • Cell Characteristics of the Lymphoid Nodules in the Peripheral Organs of Immunogenesis. Guseinova, S.; Omarova, N.; Guseinov, T. // Bulletin of Experimental Biology & Medicine;Decv2011, Vol. 152 Issue 2, p262 

    Dehydration for 3, 6, and 10 days leads to morphological changes in the small intestinal and splenic lymphoid nodules: the number of lymphoid nodules with germinative centers reduces on days 6 and 10; the percentage of blasts and mitoses decreases starting from day 3; the distance between...

  • Aeromonas hydrophila Ile Enfekte Edilen G�kkusagi Alabaligi (Oncorhynchus mykiss)'nin Gastroenteropankreatik Sisteminin Immunohistokimyasal Olarak Incelenmesi. K�pr�c�, Sibel; Sarieyy�poglu, Mustafa; Tarak�i, Berrin Gen�er // Firat University Journal of Science;Dec2009, Vol. 21 Issue 2, p89 

    A total of eighty rainbow trout were used in this study. Three experimental and one control groups were made from those fishes. Experimental groups were infected with Aeromonas hydrophila by smearing on the gills, intraperithoneal and intramuscularly injections of bacterium. Immunohistochemical...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics