Penman, I.D.; Smith, V.; Shen, E.F.; Wieand, D.; Landon, T.H.; Wong, N.A.C.S.; Lessells, A.M.; Paterson-Brown, S.; Tang, J.Z.; Wu, T.; Hillan, K.J.
April 2003
Gut;Apr2003 Supplement 1, Vol. 52, pA7
Academic Journal
Introduction and Aims: The molecular genetic events involved in the metaplasia dysplasia carcinoma (MDC) sequence in Barrett's oesophagus (CLO) are incompletely understood. We applied microarray expression analysis of endoscopic biopsies to study further these events and to detect novel genes involved in the process. Methods: Paired biopsies representing progression through Barrett's oesophagus (CLO), Iow and high-grade dysplasia (LGD, HGD), odenocarcinoma (Adca), and CLO adjacent to adenocarcinoma (Adca-BO), were taken from patients undergoing surveillance endoscopy. Biopsies were also taken from normal squamous mucosa. cDNA microarrays of 9031 genes were used to identify genes that were expressed in different disease stages. Data for each microarray were normalised to calculate Z scores and expression ratios: genes with Z scores > 1.7 (ratios 2-20) occurring in > 25% of samples from each "stage" were considered to be significantly expressed. Results: 460 genes satisfied these criteria. The mean number of expressed markers increased with progression from CLO (7.6) through LGD (11.7) to HGD (16.4). The data reveal progressive increases with dysplasia in a variety of markers involved in inflammation (eg IL-1 homologue H1, IL-17 and its receptor, chemokine receptor CXCR4, COX-2), intestinal differentiation (eg myosin MY01A, AGR2) and carcinogenesis (eg c-fos, EGFR, VEGFC), suggestive of a differentiated small intestinal enterocyte lineage, along with increased expression of TCF4. Gene expression profiles in adenocarcinoma also show evidence of Wnt-related expression, similar to colonic carcinoma. Conclusions: These results define a collection of markers that may assist in identifying patients with higher risk of developing cancer, and highlight multiple novel genes that merit further study in Barrett's.


Related Articles

  • Long-term Follow-up of Barrett's High-Grade Dysplasia. Weston, Allan P.; Sharma, Prateek; Topalovski, Margaretia; Richards, Robert; Cherian, Rachel; Dixon, Anita // American Journal of Gastroenterology;Aug2000, Vol. 95 Issue 8, p1888 

    OBJECTIVE: The management of Barrett's high-grade dysplasia (HGD) remains controversial. The aims of this study were to evaluate prospectively the outcome of unifocal HGD (uHGD) in patients with Barrett's esophagus, and to determine demographic and endoscopic features predictive of progression...

  • Endoscopy II.  // Gut;Jul2012 Supplement, Vol. 61, pA267 

    The article presents abstracts on medical topics of Great Britain which include the cost of baristric surgery for endoscopy, indications of colonoscopy and the detection of dysplasia in Barrett's oesophagus.

  • Endoscopic Ultrasound in the Evaluation of Barrett's Esophagus: A Preliminary Report. Srivastava, Amit K.; Vanagunas, Arvydas; Kamel, Perry; Cooper, Rita // American Journal of Gastroenterology;Dec1994, Vol. 89 Issue 12, p2192 

    Objective: Endoscopic screening of Barrett's esophagus (BE) for dysplasia is imprecise and controversial. Endoscopic ultrasound (EUS) allows a detailed circumferential image of the esophageal wall. Our objective in this study was to assess the utility of EUS for surveillance in Barrett's...

  • The Development of Dysplasia and Adenocarcinoma During Endoscopic Surveillance of Barrett's Esophagus. Katz, David; Rothstein, Richard; Schned, Alan; Dunn, John; Seaver, Kim; Antonioli, Donald // American Journal of Gastroenterology;Apr1998, Vol. 93 Issue 4, p536 

    Objective: Periodic endoscopic surveillance is generally recommended for patients with Barrett's esophagus. The optimal follow-up strategy for uncomplicated Barrett's esophagus is controversial, in part because of limited data on the rate of neoplastic progression (through the sequence of...

  • Is Barrett's Esophagus Associated with Intestinal Metaplasia of the Gastric Cardia? Morales, Thomas G.; Bhattacharyya, Achyut; Johnson, Cynthia; Sampliner, Richard E. // American Journal of Gastroenterology;Oct1997, Vol. 92 Issue 10, p1818 

    Objective: Barrett's esophagus has been associated with adenocarcinoma of the esophagogastric junction and gastric cardia. The purpose of this study was to determine whether patients with Barrett's esophagus have a higher prevalence of intestinal metaplasia involving the gastric cardia than...

  • The Incidence of Adenocarcinoma and Dysplasia in Barrett's Esophagus. O'Connor, J. Barry; Falk, Gary W.; Richter, Joel E. // American Journal of Gastroenterology;Aug1999, Vol. 94 Issue 8, p2037 

    OBJECTIVE: The reported incidence of adenocarcinoma in Barren's esophagus is variable. The aim of this study was to determine the incidence of dysplasia and adenocarcinoma in a population of patients with Barrett's esophagus followed prospectively and to compare these findings with other series....

  • Cost-Effectiveness of Screening and Surveillance for Barrett Esophagus.  // Annals of Internal Medicine;2/4/2003, Vol. 138 Issue 3, p41 

    Estimates the cost-effectiveness of several strategies of screening for Barrett esophagus. Simulation of what would happen to a virtual group of 50-year-old men with gastroesophageal reflux (GERD); Cost-effectiveness of screening with periodic follow-up endoscopy to detect cancer only for those...

  • GERD: Managing a troublesome condition. Stepan, Crenguta; Christina M.Surawicz // Cortlandt Forum;Nov2006, Vol. 19 Issue 11, p55 

    The article offers tips on how to treat gastroesophageal reflux disease (GERD). Reportedly, death cases of GERD is estimated to reach by about 18.6 million in the U.S., where its clinical symptoms include indigestion, atypical chest pain, and vomiting. Research shows that GERD could be treated...

  • Increased Expression of Epidermal Growth Factor Receptors in Barrett's Esophagus Associated with Alkaline Reflux: A Putative Model for Carcinogenesis. Jankowski, J.; Hopwood, D.; Pringle, R.; Wormsley, K. G. // American Journal of Gastroenterology;Mar1993, Vol. 88 Issue 3, p402 

    A 49-yr-old male was reviewed who had a 10-yr history of reflux esophagitis. He presented initially with frequent hearthurn of moderate severity and, on subsequent endoscopy, was noted to have erosive esophagitis and, at that time, a high maximal gastric acid output. During the next 5 yr, his...


Read the Article


Sign out of this library

Other Topics