Localization of Helicobacter pylori Heat Shock Protein 20

Du, R.J.; Ho, B.
September 2002
Gut;Sep2002 Supplement 2, Vol. 51, pA10
Academic Journal
Helicobacter pylori Heat Shock Protein 20 (HP20) was identified through ORF prediction in H. pylori genome sequences. It is a homologue of Hs1V protein in E. coli, which was found to be a peptidase that participates in the degradation of a cell division inhibitor, SulA. In order to characterize HP20, the gene (hp20) was cloned and inserted into expression vector pET16b fused with His-tag. The recombinant protein (rHP20) was expressed by IPTG induction in E. coli BL-21 and purified by affinity chromatography. Antibody was raised against rHP20 in rabbits. Protein A sepharose purified anti-rHP20 antibody was applied as a probe to determine the localization of HP20 in H. pylori cells by immuno-gold labeling. Simultaneously, rHP20 was also used as H. pylori antigen to test against sera obtained from patients with different gastroduodenal diseases. The results from Western Blotting and immuno-gold labeled transmission electron microscopy show that HP20 is localized on cell surface of H. pylori and mainly expressed in spiral-shaped H. pylori. ELISA shows both H. pylori infected and non-infected sera were seropositive to rHP20. The cross-reaction could be due to earlier exposure of patients to HP20 homologues from other bacteria, e.g. HslV of E. coli. However, the mechanism has yet to be determined. Could it be that HP20 emulates HSP60 as molecular mimicry agent in the host? The surface localization of HP20 on Helicobacter pylori spiral serves to lead the way to understanding Helicobacter pylorihost interaction.


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