TITLE

Identification of a locus associated with cell integrity in Helicobacter pylori by transposon shuttle random mutagenesis

AUTHOR(S)
Chuang, Y.; Lin, T.; Sun, C.; Chang, K.; Wang, J.
PUB. DATE
September 2002
SOURCE
Gut;Sep2002 Supplement 2, Vol. 51, pA9
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To identify genes involved in adhesion, we generated 1500 insertion mutants of a Helicobacter pylori strain by transposon shuttle mutagenesis. All mutant strains were screened for their adhesion ability with cultured gastric cancer epithelial cell line (SC-MI). Six mutants exhibited 10-fold decrease of recovered adhered bacteria number than H. pylori wild-type strain after co-incubated with SC-MI cell for 30 minutes. DNA sequencing revealed that the interrupted loci of these six mutants are the same. The sequence of this gene does not have homologues with sequenced H. pylori 26695 and J99 strains. This novel open reading frame contains 1491 base pairs. Light microscopic observations revealed that these mutant cells were self-aggregated frequently. This mutant also revealed cell elongation under electron microscopic examination. Complementation of this locus turned the morphology into the same as wild type. Therefore, this novel ORF may be involved in H. pylori structure integrity.
ACCESSION #
9747261

 

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