TITLE

ISOLATION AND CHARACTERISATION OF HUMAN HEPATIC STEM CELLS IN FETAL LIVER

AUTHOR(S)
Currie, I.S.; Masson, N.M.; Dundas, S.R.; Black, J.R.; Bayne, R.A.L.; Parks, R.W.; Garden, O.J.; Ross, J.A.
PUB. DATE
April 2003
SOURCE
Gut;Apr2003 Supplement 1, Vol. 52, pA1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction: Hepatic stem cells have been described in rat which express the surface antigens CD90 (Thy-1) and CD34. We hypothesised that a similar population may exist in human fetal liver, giving rise to hepatocyte precursors after cytokine treatment in vitro. Methods: Human fetal liver was subject to collagenase digestion. The cells were labelled with anti-CD90 monoclonal antibody, anti-mouse phycoerythrin conjugate and FITC-conjugated monoclonal anti-CD34 antibody. Fluorescence-activated cell sorting provided CD90+ve, CD90-ve/CD34+ve or CD90+ve/CD34+ve cells, which were cultured for 7 days in the presence and absence of cytokines (stem cell factor, thrombopoietin and FIt3-ligand). Cultures were fixed and immunostained to show biliary tract (cytokeratin 19 (CK19)) and hepatocyte (cytokeratin 18 (CK18), Fibrinogen (FIB)) markers. Results: Two per cent of the liver cell suspension was CD90+ve, 9% was CD34+ve/CD90-ve, and 2% was CD34+ve/CD90+ve. By day 7 in vitro, CD34+ve cells were absent. By contrast, CD90+ve cells were seen in cultures derived from CD90+ve cells and, surprisingly, from CD34+ve/CD90-ve cells. In cultures prepared from CD34+ve/CD90+ve cells, survival depended on cytokines. Many small, fibrinogen-ve, CK19+ve cells and other slightly larger cells staining for CK18 were noted. In cultures of CD34+ve/CD90-ve cells, we observed cells positive for FIB or CK19. In CD90+ve cell cultures, cytokines had little effect. Occasional cells with hepatocyte morphology, which were positive for FIB and CK19 or CK18, were seen in these cultures alone. Conclusion: Cultures of purified human hepatic stem cells showed that CD90+ve cells can produce bipotential hepatocyte/biliary tract (FIB+ CK19+) cells and monopotential hepatocytes (FIB, CK18). Conversely, other stem cell types may produce earlier liver cell precursors.
ACCESSION #
9747250

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sign out of this library

Other Topics