Progression of Coxiella burnetii infection after implementing a two-year vaccination program in a naturally infected dairy cattle herd

Piñero, Alvaro; Barandika, Jesús F.; Hurtado, Ana; García-Pérez, Ana L.
August 2014
Acta Veterinaria Scandinavica;2014, Vol. 56 Issue 1, p1
Academic Journal
Background The high prevalence of Coxiella burnetii infection in dairy cattle herds recently reported and the long survival time of the bacterium in the environment pose a risk to human and animal health that calls for the implementation of control measures at herd level. This study presents the results of a 2-year vaccination program with an inactivated phase I vaccine in a Spanish dairy herd naturally infected with C. burnetii. Calves older than 3 months and non-pregnant heifers and cows were vaccinated in April 2011 and the farm was subsequently visited at a monthly basis for vaccination of recently calved cows and calves that reached the age of 3 months. Annual booster doses were given to previous vaccinated animals as well. The effectiveness of the vaccine was assessed in terms of level of C. burnetii shedding through milk and uterine fluids and environmental contamination as determined by polymerase chain reaction (PCR). Results The percentage of shedder animals through uterine fluids and milk progressively decreased, and C. burnetii DNA load in bulk-tank milk samples was low at the end of the study. The average seroconversion rate in not yet vaccinated animals, which acted as control group, was 8.6% during the first year and 0% in the second year. DNA of C. burnetii was found in aerosols and dust samples taken in the calving area only at the beginning of the study, whereas slurry samples remained C. burnetii PCR positive for at least 18 months. Multiple Locus Variable number tandem-repeat Analysis identified the same genotype in all C. burnetii DNA positive samples. Conclusions In the absence of any changes in biosecurity, the overall reduction of C. burnetii infection in animals to 1.2% milk shedders and the reduced environment contamination found at the end of the study was ascribed to the effects of vaccination together with the culling of milk shedders. Vaccination has to be planned as a medium-long term strategy to suppress risks of re-infection.


Related Articles

  • Coxiella burnetii in bulk tank milk samples from dairy goat and dairy sheep farms in the Netherlands in 2008. den Brom, R. van; van Engelen, E.; Luttikholt, S.; Moll, L.; van Maanen, K.; Vellema, P. // Veterinary Record: Journal of the British Veterinary Association;3/24/2012, Vol. 170 Issue 12, p310 

    The article presents a study on the prevalence of Q fever among samples of dairy goat and sheep bulk tank milk (BTM) in the Netherlands. The study used various methods such as real-time polymerase chain reaction (RT-PCT), enzyme-linked immunosorbent assay (ELISA) and immunohistorchemistry (IHC)....

  • Spread of Coxiella burnetii infection in a flock of sheep after an episode of Q fever. Berri, M.; Crochet, D.; Santiago, S.; Rodolakis, A. // Veterinary Record: Journal of the British Veterinary Association;12/3/2005, Vol. 157 Issue 23, p737 

    The article investigates the prevalence of Coxiella burnetii infection in the sheep flock following an episode of Q fever. The ewes were treated with oxytetracycline to reduce the shedding of C. burnetii and the prevent further abortions. with this regard, the seroprevelance of C. burnetii in...

  • Coxiella burnetii in samples from cattle herds and sheep flocks in the Kars region of Turkey. SAGLAM, A. GULMEZ; SAHIN, M. // Veterinární Medicína;2016, Vol. 61 Issue 1, p17 

    This study was aimed at determining the presence of C. burnetii in cattle and sheep herds in the Kars region of Turkey using serological and molecular methods. As a serological technique, C.burnetii IgG in blood sera and milk samples were investigated with ELISA. The results of these...

  • Cysteinyl leukotriene signaling through perinuclear CysLT receptors on vascular smooth muscle cells transduces nuclear calcium signaling and alterations of gene expression. Eaton, Alison; Nagy, Edit; Pacault, Mathilde; Fauconnier, Jérémy; Bäck, Magnus // Journal of Molecular Medicine;Oct2012, Vol. 90 Issue 10, p1223 

    Leukotrienes are pro-inflammatory mediators that are locally produced in coronary atherosclerotic plaques. The response induced by cysteinyl leukotrienes (CysLT) in human coronary arteries may be altered under pathological conditions, such as atherosclerosis. The aim of the present study was to...

  • Allicin Protects against Lipopolysaccharide-Induced Acute Lung Injury by Up-Regulation of Claudin-4. Yue-liang Zheng; Wen-wei Cai; Guang-zhao Yan; Yuan-zhan Xu; Mei-qi Zhang // Tropical Journal of Pharmaceutical Research;Jul2014, Vol. 13 Issue 7, p1063 

    Purpose: To investigate the effect of allicin, an active component of garlic, on lipopolysaccharide (LPS)-induced acute lung injury. Methods: Wistar rats were subjected to LPS intravenous injection with or without allicin treatment to induce acute lung injury (ALI) model. Also, A549 cells were...

  • Immuno-PCR for the early serological diagnosis of acute infectious diseases: the Q fever paradigm. Malou, N.; Renvoise, A.; Nappez, C.; Raoult, D. // European Journal of Clinical Microbiology & Infectious Diseases;Aug2012, Vol. 31 Issue 8, p1951 

    To reduce the delay in diagnosis of Q fever, we have adapted the ultrasensitive immuno-PCR method for the detection of Phase II IgM anti- Coxiella burnetii. We compared its performance to ELISA, IFA and PCR using 31 acute Q fever sera and 50 control sera. The best sensitivity was obtained by...

  • Modulation of lipopolysaccharide-induced neuronal response by activation of the enteric nervous system. Coquenlorge, Sabrina; Duchalais, Emilie; Chevalier, Julien; Cossais, Francois; Rolli-Derkinderen, Malvyne; Neunlist, Michel // Journal of Neuroinflammation;2014, Vol. 11 Issue 1, p53 

    Background Evidence continues to mount concerning the importance of the enteric nervous system (ENS) in controlling numerous intestinal functions in addition to motility and epithelial functions. Nevertheless, little is known concerning the direct participation of the ENS in the inflammatory...

  • Polymerase Chain Reaction Detection of Aspergillus DNA in Experimental Models of Invasive Aspergillosis. Loeffler, Juergen; Kloepfer, Kerstin; Hebart, Holger; Najvar, Laura; Graybill, John R.; Kirkpatrick, William R.; Patterson, Thomas F.; Dietz, Klaus; Bialek, Ralf; Einsele, Hermann // Journal of Infectious Diseases;4/15/2002, Vol. 185 Issue 8, p1203 

    To determine the sensitivity of polymerase chain reaction (PCR) assays for the diagnosis of invasive aspergillosis, results of quantitative culture, PCR-ELISA, and a quantitative LightCycler assay (Roche Diagnostics) of blood and organ specimens of experimentally infected mice and rabbits ...

  • Comprehensive in vivo Mapping of the Human Basal Ganglia and Thalamic Connectome in Individuals Using 7T MRI. Lenglet, Christophe; Abosch, Aviva; Yacoub, Essa; De Martino, Federico; Sapiro, Guillermo; Harel, Noam // PLoS ONE;Jan2012, Vol. 7 Issue 1, p1 

    Basal ganglia circuits are affected in neurological disorders such as Parkinson's disease (PD), essential tremor, dystonia and Tourette syndrome. Understanding the structural and functional connectivity of these circuits is critical for elucidating the mechanisms of the movement and...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics