TITLE

HPTLC determination of Gallic acid in Methanol extract of Quercus griffithii Acorn

AUTHOR(S)
Tsering, Jambey; Gogoi, Baikuntha J.; Veer, Vijay; Kalita, Pallabi; Tag, Hui
PUB. DATE
August 2014
SOURCE
International Journal of PharmTech Research;Aug/Sep2014, Vol. 6 Issue 4, p1341
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This paper describes HPTLC determination of gallic acid in methanol extract of Q. griffithii acorn. Macerated extract solution of Q. griffithii acorn was applied on HPTLC plate along with standard by using Camag Linomat-5. The detection of gallic acid was performed on aluminium plates pre-coated with silica gel 60 F254 as the stationary phase. Optimized mobile phase toluene: ethyl acetate: formic acid (6:6:1) was used. Plate was developed in twin trough chamber and scanned by TLC scanner III at lmax i.e. 297 nm. The system was found to give compact spots for gallic acid at Rf 0.41. Amount of gallic acid determined in 1 Kg dry acorn powder of Q. griffithii was found to be 0.19±0.01 g. The method was validated in terms of sensitivity, precision, specificity, robustness and recovery. Edibility of acorn and presence of gallic acid in Q. griffithii is reporting for the first time.
ACCESSION #
97368541

 

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