Variations of Stem Anatomy of Cabbage Seedlings (Brassica oleracea L.) Seedlings Produced from Heat-Shock Callus Tissue

Rasheed, Jamella H.; Alradi, Faiq H.; Alzeadan, Sahla M.
July 2014
Australian Journal of Basic & Applied Sciences;Jul2014, Vol. 8 Issue 10, p383
Academic Journal
Background: The current study concentrated from an effect of heat- shock exposure of callus derived from stem segments of cabbage plants (Brassica olerea L.). Objective: The heat shock (20,25,30,35 and 40 C°) for 10 and 20 minutes for each degree increased callus growth, size and easy to regenerate the callus to shoot and root. Results: Regeneration ratio reach up to 91.66%, especially in temperature 40 C° for 10 minutes, and the numbers of shoots were 13, while in the same degree for 20 minutes the ratio of regeneration was 44.23% and the shoots were 2. On the other hand the anatomical characteristics are different in the shape, numbers of vascular bundles and the pith area. Conclusion: This study aimed to identify the anatomical variation in stem of seedlings produced from callus exposed to heat-shock.


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