TITLE

The Profile of Gill Protein Expression of Humpback Grouper (Cromileptes Altivelis) Injected with Per-Cp of Halimeda Opuntia and Viral Nervous Necrosis

AUTHOR(S)
Dahoklory, Nicodemus; Yanuhar, Uun; Sukoso; Permana, Sofi
PUB. DATE
July 2014
SOURCE
Australian Journal of Basic & Applied Sciences;Jul2014, Vol. 8 Issue 10, p145
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The objectives of the research are to obtain the protein Per-CP Halimeda opuntia and to understand the cellular expression of the gill of the Humpback grouper (Cromileptes altivelis). The isolate of protein Per-CP H. opuntia is stored at -800C. Humpback grouper is assigned into three groups, which are normal grouper, grouper injected with protein Per-CP H. opuntia, and grouper injected with Per-CP H. opuntia and challengetested with VNN virus. The gill is taken out, charged with liquid Nitrogen and stored at -800C. The Result of research show that injection of Per-CP of H.opuntia into humpback grouper was able to increase protein expression of gill of Humpback grouper at molecule weight of 56 kDa and 5 kDa. By using western blot methods, it was clear that protein Per-CP of H.opuntia act as antiviral VNN expressed through protein 5 kDa of the gill organ
ACCESSION #
97368472

 

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