Production of Pure Hinai-dori with Normal Reproductive Capability from Transferred Primordial Germ Cells

Kazuhiro Rikimaru; Yoshiaki Nakamura; Daiki Takahashi; Megumi Komatsu; Natsuki Ito; Kazuei Matsubara; Takahiro Tagami
July 2014
Journal of Poultry Science;Jul2014, Vol. 51 Issue 3, p297
Academic Journal
The production method of germline chimeras by transfer of primordial germ cells (PGCs) can be used in gene banking of chickens. However, little is known about the reproductive capability of offspring obtained by mating of male and female germline chimeras. To cryopreserve chicken genetic resources at the cellular level in future, it is necessary to demonstrate whether offspring originating from donor-derived PGCs have normal reproductive capability. In this study, we attempted to obtain the pure Hinai-droi offspring by mating of male and female germline chimeras produced by the transplantation of Hinai-dori PGCs into the Akita White Leghorn line (WL) embryos. Hinai-dori offspring from germline chimeras were screened using the Hinai-dori-specific autosomal microsatellite marker, namely, ADL0315, which allows distinction between the Hinai-dori breed and Akita WL. After sexual maturity of the Hinai-dori offspring originating from donor-derived Hinai-dori PGCs, the reproductive capability (egg production rate, fertilization rate and hatchability) was examined. Percentage hen-day egg production was investigated between 21 and 60 wk of age, and fertilization rate and hatchability were investigated between 43 and 60 wk of age, respectively. Among the 105 progeny obtained by mating of male and female germline chimeras, a single brown-colored female hatchling was obtained. The results from molecular genotyping using the Hinai-dori-specific microsatellite marker ADL0315 were consistent with results from morphological identification, and the brown-colored female hatchling was identified as the pure Hinai-dori breed. The mean percentage hen-day egg production, fertilization rate and hatchability of the Hinai-dori hen were 36.4%, 58.1% and 53.2%, which were similar to those of normal Hinai-dori hens. These results indicated that the pure Hinai-dori offspring originating from donor-derived Hinai-dori PGCs has normal reproductive capability.


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